Novel Biosensors Using Electropolymerized Film

使用电聚合薄膜的新型生物传感器

• 批准号: 09650912
• 负责人: OSAKA Tetsuya
• 金额: $ 0.96万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650912/
• 关键词: Electropolymerization; Polypyrrole; Biosensor; Polyion complex; Urea sensor; Flow injection analysis; Molecular sieving; pH-stat; 酵素阻害; バイオセンサ; 尿素; クレアチニン; グルコース; 酵素電極

This study was done aiming to pile the basic knowledge on the novel biosensors and to establish the design of new functional interface with the investigation of biosensing devices using electropolymerized films. Especially, in this study, an electropolymerized inactive polymer film was applied to fabricate the biosensors, and it is a new attempt to use for the sensors.From the study, the combination of electropolymerized inactive polypyrrole film and film formation method of polyion complex (PlC) is cleared to be an effective way to incorporation and entrapment of enzyme in the functional film. In addition, it was clarified that this sensor is applicable to the flow injection analysis. Based on these results, a new biosensor for creatinine was designed and fabricated. The system using electropolymerized inactive polypyrrole and PlC for a creatinine sensing is well applicable to the sensing system whose enzyme reaction causes a pH change. In addition, with these results, molecular sieving effects of PlC were studied. This enables us to make a variation of sensitivity for the sensors with the PlC structure.In conclusion, the basic phenomena of biosensors using electropolymerized inactive polymer and PlC were revealed and new approach for novel sensing system was proposed.

本研究的目的是通过对电聚合膜生物传感器的研究，积累新型生物传感器的基础知识，并建立新型功能界面的设计。特别是本研究将电聚合的非活性聚合物膜应用于生物传感器的制备，是一种新的尝试，研究表明，电聚合的非活性聚吡咯膜与聚离子复合物（PlC）成膜方法相结合，是一种将酶包埋在功能膜中的有效方法。此外，还阐明了该传感器适用于流动注射分析。在此基础上，设计并制备了一种新型的肌酸酐生物传感器。使用电聚合的非活性聚吡咯和PLC用于肌酐传感的系统很好地适用于其酶反应引起pH变化的传感系统。此外，利用这些结果，研究了PLC的分子筛效应。总之，揭示了电聚合非活性聚合物与PLC生物传感器的基本现象，为新型传感系统的研究提供了新的思路。

项目成果

T.Osaka: "Highly Sensitive Microbiosensor for Creatinine Based on the Combination of Inactive Polypyrrole with Polyion Complexes" J.Electrochem.Soc.145 (2). 406-408 (1998)

T.Osaka：“基于非活性聚吡咯与聚离子复合物组合的肌酸酐高灵敏微生物传感器”J.Electrochem.Soc.145 (2)。

S.Komaba: "Impedance Analysis of Electrodeposited Insulating Polypyrrole" J.Electroanal.Chem.453. 19-23 (1998)

S.Komaba：“电镀绝缘聚吡咯的阻抗分析”J.Electroanal.Chem.453。

T.Osaka: "Creatinine Microbiosensor Using Electroinactive Polypyrrole with Various Polyion Complexes" Technical Digest of the Seventh International Meeting on Chemical Sensors. 247-249 (1998)

T.Osaka：“使用电惰性聚吡咯和各种聚离子复合物的肌酸酐微生物传感器”第七届国际化学传感器会议技术摘要。

S.Komaba: "Flow Injection Analysis of Potassium Using an All-Solid-State Potassium-Selective Electrode as a Detector" Talanta. 46. 1293-1297 (1998)

S.Komaba：“使用全固态钾选择性电极作为检测器对钾进行流动注射分析”Talanta。

T.Osaka: "High Sensitivity Flow Injection Analysis of Urea Using Composite Electropolymerized Polypyrrole-Polyion Complex Film" J.Electrochem.Soc.146(2). 615-619 (1999)

T.Osaka：“使用复合电聚合聚吡咯-聚离子复合膜对尿素进行高灵敏度流动注射分析”J.Electrochem.Soc.146(2)。