Novel Biosensors Using Electropolymerized Film

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This study was done aiming to pile the basic knowledge on the novel biosensors and to establish the design of new functional interface with the investigation of biosensing devices using electropolymerized films. Especially, in this study, an electropolymerized inactive polymer film was applied to fabricate the biosensors, and it is a new attempt to use for the sensors.From the study, the combination of electropolymerized inactive polypyrrole film and film formation method of polyion complex (PlC) is cleared to be an effective way to incorporation and entrapment of enzyme in the functional film. In addition, it was clarified that this sensor is applicable to the flow injection analysis. Based on these results, a new biosensor for creatinine was designed and fabricated. The system using electropolymerized inactive polypyrrole and PlC for a creatinine sensing is well applicable to the sensing system whose enzyme reaction causes a pH change. In addition, with these results, molecular sieving effects of PlC were studied. This enables us to make a variation of sensitivity for the sensors with the PlC structure.In conclusion, the basic phenomena of biosensors using electropolymerized inactive polymer and PlC were revealed and new approach for novel sensing system was proposed.