Basic study on DNA vaccine in Hepatitis type C

丙型肝炎DNA疫苗基础研究

• 批准号: 09670568
• 负责人: MORIYAMA Takashi
• 金额: $ 2.11万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670568/
• 关键词: hepatitis C virus; DNA vaccine; cytotoxic T lymphocyte; adjuvant; アジュバント; 慢性肝炎; 肝硬変; 肝細胞癌; C型肝炎ウイルス; 細胞障害性T細胞; 免疫学

We tried to induce a T cell liesponse to hepatitic Cvirus (HCV) core protein by using a DNA vaccine in mice. We constructed plasmids encoding HCV core region under the control of various promotors such as pMAMneo, pEFBOS, and pcDNA3. These plasmids alone induced a helper T cell response only after in vitro restimulation twice. A cytotoxic Tlymphocyte (CTL) response was observed but the magnitude was not strong. Next we immunized mice with a plasmid encoding HCV core region together with an adjuvant plasmid encoding IL-2, GM-CSF, or (CD86. None of these combination resulted in a betterT cell response yet.

我们尝试用DNA疫苗在小鼠体内诱导T细胞对丙型肝炎病毒（HCV）核心蛋白的应答。我们构建了在各种启动子如pMAMneo、pEFBOS和pcDNA 3控制下编码HCV核心区的质粒。这些质粒仅在体外再刺激两次后才诱导辅助性T细胞应答。观察到细胞毒性T淋巴细胞（CTL）反应，但幅度不强。接下来，我们用编码HCV核心区的质粒以及编码IL-2、GM-CSF或CD 86的佐剂质粒免疫小鼠。这些组合都没有导致更好的T细胞反应。

项目成果

Ando, Kazuki.: "Perforin, Fas/Fas ligand, TNF-alpha pathways as specific and bystander killing mechanisms of hepatitis C virus-specific human CTL" Journal of Immunology. 158. 5283-5291 (1997)

Ando, Kazuki.：“穿孔素、Fas/Fas 配体、TNF-α 途径作为丙型肝炎病毒特异性人类 CTL 的特异性和旁观者杀伤机制”免疫学杂志。

Kaneko, Takashi: "Impaired induction of cytotoxic T lymphocytes by antagonism of a weak agonist borne by a variant hepatitis C virus epitope" European Journal of Immunology. 27. 1782-1787 (1997)

Kaneko, Takashi：“丙型肝炎病毒变异表位所携带的弱激动剂的拮抗作用会损害细胞毒性 T 淋巴细胞的诱导”，《欧洲免疫学杂志》。

Ando, Kazuki: "Perforin, Fas/Fas ligand, TNF-a pathways as specific and bystander killing mechanisms of hepatitis C virus-specfic human CTL" Jornal of Immunology. 158. 5283-5291 (1997)

Ando, Kazuki：“穿孔素、Fas/Fas 配体、TNF-a 途径作为丙型肝炎病毒特异性人 CTL 的特异性和旁观者杀伤机制”《免疫学杂志》。

Hiroishi, Kazumasa.: "Cytotoxic T lymphocyte response and viral load in hepatitis C virus infection." Hepatology. 25. 702-712 (1997)

Hiroishi, Kazumasa.：“丙型肝炎病毒感染中的细胞毒性 T 淋巴细胞反应和病毒载量。”

Kaneko,Takashi: "Impaired induction of cytotoxic T lymphocytes by antagonism of a weak agonist borne by a variant hepatitis C virus epitope" European Journal of Immunology. 27. 1782-1787 (1997)

Kaneko，Takashi：“丙型肝炎病毒表位变异所携带的弱激动剂的拮抗作用损害了细胞毒性 T 淋巴细胞的诱导”《欧洲免疫学杂志》。