Basic study on Enhancement Effect on DNA vaccine in Hepatitis type C

丙型肝炎DNA疫苗增强作用的基础研究

• 批准号: 11670528
• 负责人: MORIYAMA Takashi
• 金额: $ 2.3万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670528/
• 关键词: hepatitis C virus; DNA vaccine; cytotoxic T lymphocyte; dendritic cell

We tried to induce a T cell response to hepatitic C virus (HCV) NS5A protein by using a DNA vaccine in mice. We immunized mice with a plasmid encoding HCV NS5A region alone or with an adjuvant plasmid encoding IL-2, GM-CSF, or CD86. Neither of them induced a cytotoxic T lymphocyte (CTL) response of a siginificant magnitude.Next we induced mature dendritic cells with IL-4 and GM-CSF from bone marrow cells. We immunized mice with dencritic cells pulsed with reportedly immunogenic CTL epitopes. None of these mice showed a better T cell response yet.

我们尝试用DNA疫苗在小鼠体内诱导T细胞对丙型肝炎病毒NS5A蛋白的应答。我们用编码丙型肝炎病毒NS5A区的质粒单独或用编码IL-2、GM-CSF或CD86的佐剂质粒免疫小鼠。接下来，我们用IL-4和GM-CSF从骨髓细胞中诱导成熟的树突状细胞。我们用据称具有免疫原性CTL表位脉冲的树突状细胞免疫小鼠。这些小鼠都没有表现出更好的T细胞反应。

项目成果

Honda,A.: "HCV-core protein accelerates recovery from the insensitivity of liver cells to Fas-mediated apoptosis in mice"J.Hepatology. (In Press).

Honda,A.：“HCV 核心蛋白可加速小鼠肝细胞对 Fas 介导的细胞凋亡不敏感的恢复”J.Hepatology。

Honda,A., et al.: "HCV-core protein accelerates recovery from the insensitivity of liyer cells to Fas-mediated apoptosis in mice."Journal of Hepatology. 33. 440-447 (2000)

Honda,A. 等人：“HCV 核心蛋白可加速小鼠从对 Fas 介导的细胞凋亡不敏感的细胞中恢复。”肝脏病学杂志。

Honda,A., et al.: "HCV-core protein accelerates recovery from the insensitivity of liver cells to Fas-mediated apoptosis in mice."Journal of Hepatology. 33. 440-447 (2000)

Honda,A. 等人：“HCV 核心蛋白可加速小鼠肝细胞对 Fas 介导的细胞凋亡不敏感的恢复。”肝脏病学杂志。

Honda,A.: "Hepatitis C virus structural proteins induce liver cell injury in transgenic mice"J.of Med.Virol.. 59. 281-289 (1999)

Honda,A.：“丙型肝炎病毒结构蛋白诱导转基因小鼠肝细胞损伤”J.of Med.Virol.. 59. 281-289 (1999)