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Role of the gastric mucus on the Helicobacter pylori infection.

胃粘液对幽门螺杆菌感染的作用。

基本信息

批准号：
09670572
负责人：
HOTTA Kyoko
金额：
$ 1.73万
依托单位：
KITASATO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670572/
关键词：
Helicobacter pylori Mucus gel Monoclonal antibody Gastric mucus mucin Gastric mucosa Dot-blot ドットブロット

项目摘要

The effects of the gastric mucus on Helicobacterpylori (Hp) infection have been studied.1. The gastric mucins were purified by Lysine-Sepharose chromatography from the gastric juice which were obtained from the individuals having blood group A, B, AB or 0. The inhibition assay by the mucins in the red blood cell agglutination caused by Hp showed that the gastric mucins were potent inhibitor. The results indicated that Np bound to the gastric mucins obtained from the gastric juice. The sialydase treatment of the mucins caused the loss of the binding of the mucins to Hp ; therefore, the sialic acid residue was needed for the binding. The blood type was not correlate with the binding.2. The gastric mucins were purified from the human gastric mucosa. The methods were authorized from the experiments using the rat gastric mucosa. The purified human gastric mucins were separated to the surface and glandular mucosa-specific mucins using the gel-filtration and ion-exchange chromatography. The dot-blot analysis and ELISA using monoclonal antibody HIK1083 were used to detect the individual mucin. The mucins from the surface mucosa were different from those from the glandular mucosa on their size. Although the complete separation of both mucins have not yet been done, the crude mucins bound to Hp. The mucins from the antrum bound strongly to Hp compared to those from the corpus.3. The gastric mucins were prepared from the rat and mouse stomach and they were compared to the human mucins on the binding capacity to Hp. The rat or mouse gastric mucins bound only slightly to Hp. The differences of the structure among the mucins have been characterized.

本文研究了胃粘液对幽门螺杆菌（Helicobacterpylori，Hp）感染的影响.用赖氨酸-琼脂糖色谱法从A、B、AB或0型血个体的胃液中纯化胃粘蛋白。胃粘蛋白对Hp引起的红细胞凝集反应的抑制试验表明，胃粘蛋白对Hp引起的红细胞凝集反应有较强的抑制作用。结果表明，Np与从胃液中获得的胃粘蛋白结合。粘蛋白的唾液酸酶处理导致粘蛋白与Hp结合的丧失;因此，结合需要唾液酸残基。血型与结合无相关性.从人胃粘膜中纯化胃粘蛋白。这些方法是从使用大鼠胃粘膜的实验中获得授权的。利用凝胶过滤和离子交换层析将纯化的人胃粘蛋白分离为表面粘蛋白和腺粘膜特异性粘蛋白。采用斑点杂交法和单克隆抗体HIK 1083的ELISA法检测单个粘蛋白。结果表明，粘膜表面粘蛋白的大小与腺粘膜粘蛋白不同。虽然这两种粘蛋白的完全分离还没有完成，但粗粘蛋白与Hp结合。胃窦粘蛋白与Hp的结合较强.从大鼠和小鼠胃中制备胃粘蛋白，并将其与人粘蛋白对Hp的结合能力进行比较。大鼠或小鼠胃粘蛋白仅与Hp轻微结合。对粘蛋白的结构差异进行了表征。