How dose the gastric surface mucus cell line derived from transgenic mouse synthesize and secrete mucins?

转基因小鼠胃表面粘液细胞系如何合成和分泌粘蛋白？

• 批准号: 07680773
• 负责人: HOTTA Kyoko
• 金额: $ 1.41万
• 依托单位: KITASATO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680773/
• 关键词: Hyaluronan; Gastric mucosa; Gastric epithelial cells; Transgenic mouse; SV40; large T-antigen; GSM06 cells; ムチン; 粘液細胞; 胃粘液; モノクロナール抗体; 胃表層粘液細胞

GSM06 is a cell line established from the stomach of transgenic mouse harboring a temperature-sensitive simian virus 40 (SV40) large T-antigen gene. ^3H-Labeled macromolecules produced by the cells incubated with [^3H] glucosamine were characterized to examine whether or not GSM06 cells synthesize mucin (mucus glycoprotein). The GSM06 cells grew until a confluent monolayr formed at 33ﾟC (the permissive temperature for SV40 large T-antigen expression), and the ^3H-labeled macromolecules appeared in both cell extract and medium during culture for at least one week. Unexpectedly, almost all ^3H-labeled macromolecules, which were excluded from a column of Sepharose CL-4B,were identified as hyaluronan by analyzes using Sepharose CL-2B chromatography, cesium trifluoroacetate equilibrium centrigugation, treatment with dithiothreitol, and trypsin and hyaluronidase digestion. At a nonpermissive temperature (39ﾟC), GSM06 cells grew only slightly, but produced much more hyaluronan than at 33ﾟC.The results indicate that GSM06 cells do not produce mucin, but hyaluronan, and that the expression of large T-antigen may influence hyaluronan synthesis in GSM06 cells.

GSM06是从具有温度敏感的邻居病毒40（SV40）大型T抗原基因的转基因小鼠摊位建立的细胞系。对^3H标记的大分子由[^3H]葡萄糖胺孵育的细胞产生，以检查GSM06细胞是否合成粘蛋白（粘液糖蛋白）。 GSM06细胞生长，直到在33°C处形成的汇合单层（SV40 SV40大型T抗原表达的允许温度），并在培养过程中培养过程中出现 ^3H标记的大分子至少一周。出乎意料的是，通过使用Sepharose CL-2B色谱，三氟二氟乙酸硫酸葡萄酸酯等效式的分析，将几乎所有 ^3H标记的大分子被排除在Sepharose CL-4B的列中，被鉴定为透明质酸。在非疗法温度（39 c）下，GSM06细胞仅略有生长，但产生的透明质酸比33°C时更大。结果表明，GSM06细胞不产生粘蛋白，而是透明质酸，而大型T-抗原的表达可能会影响GSM06细胞中GSM06细胞中的透明质酸合成。

项目成果

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Kazuhiko Ishihara 等人：“针对分布在大鼠胃粘膜不同部位和层的胃粘蛋白碳水化合物部分的单克隆抗体的建立”Glycoconj.J.13。

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Watanabe T. 和其他 4 人：“维生素 U 对大鼠乙醇诱导的胃粘膜损伤的细胞保护机制”Dig.Dis.Sci.49-54 (1996)。

Yukinobu Goso他3名: "Effects of traditional herbal medicine on gastric mucin against ethanol-induced gastric injury in rats" Comp.Biochem.Phsiol.C. 113. 17-21 (1996)

Yukinobu Goso 等 3 人：“传统草药对胃粘蛋白对大鼠乙醇诱导胃损伤的影响”Comp.Biochem.Phsiol.C. 113. 17-21 (1996)

K. Ishihara他4名: "A Monoclonal Antibody to the Gastric Gland Mucous Cell-Derived Mucin, HIK-1083, Reacts with Oligosaccharides Bearing Peripheral α-Linked GlcNAc Residues" Glycoconj. J.12. 504- (1995)

K. Ishihara 和其他 4 人：“胃腺粘液细胞衍生粘蛋白的单克隆抗体，HIK-1083，与带有外周 α-连接 GlcNAc 残基的寡糖发生反应”Glycoconj 504- (1995)。

Susumu Ohara et al.: "Gastric Mucosal Damage Accompanying Changes in Mucin Induced by Histamine in Rats" Pharmacol.Toxicol.77. 397-401 (1995)

Susumu Ohara 等人：“大鼠组胺诱导的胃粘膜损伤伴随粘蛋白变化”Pharmacol.Toxicol.77。