Treatment of bone defect with an artificial membrane of TCP/CPLA complex in a rabbit ulner model

TCP/CPLA复合物人工膜治疗兔尺骨模型骨缺损

• 批准号: 09671474
• 负责人: MUNETA Takeshi
• 金额: $ 2.05万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671474/
• 关键词: beta-tricalcium phosphate (P・TCP); Copolymer of poly-L-lactic acid (CPLA); beta-TOP; CPLA complex; Bioabsor bablemembrame; Guide bone regeneration (GBR); Treatment of bone defect; Rabbit ulner model; Soft X-ray analysis; Sリン酸三カルシウム(STCP); βTCP /

Purpose An experimental study using a rabbit ulnar bone defect model was performed to investigate the efficacy of artificial bone menbrane formed by tricalcium phosphate/copolymer of lactic acid (TCP/CPLA) complex. TCP is a well known bone conducting material and TCP/CPLA complex is easy to form in membranous structure. The second purpose of this study was to investigate the affect of aging on bone formation using the model.Methods The young group consised of 10 Japanese White Rabbits (JWR) (Body weight : 2.5-3.0kg) and the aged group consisted of 4 JWR (Body weight more than 4kg). A 1.5cm central bone defect of ulna including bone membrane was bilaterally created. The bone defects was covered with an artificial bone membrane of TCP/CPLA in one side and with a membrane of CPLA in the other side. Two JWR were sacrificed 4 weeks postoperatively ; six at 8 weeks ; and 2 at 12 weeks in the young group. All 4 rabbits were sacrificed at 8 weeks in the aged group. Soft X-ray analysis was perf … More ormed for the specimens, then histologic section was made with hematoxyline and eosin stain and Azam stain. Semi-quantitative analysis was performed using image analysis-software.Results Young group : Bony bridg between both ends of defect was completed under soft X-ray image in all specimens in the TCP/CPLA group 8 weeks postoperatively. On the other hand, bony bridg was incompleted in two specimens in the CPLA group 8 weeks postoperatively. Histologically, bone formation was recognized in the TCP/CPLA group, but one of the CPLA group showed no bone formation 4 weeks postoperatively. Bony bridg was recognized in both TCP/CPLA and CPLA groups 12 weeks postoperatively. Histology with Azam stain showed % bode formation in the TCP/CPLA group and % in the CPLA group, which was statistically significant difference. Aged group : Histologic study as well as soft x-ray image showed complete bony bridge in only one specimen of the TCP/CPLA group. Otherwise, no complete bony bridg was found in the other specimens.Discussion and Conclusion Treament of bone defect with an artificial bone membrane of TCP/CPLA complex showed a possibility of clinical use based on the results of this study. However, this membrane can not expect sufficient bone formation for aged patients because the TCP/CPLA complex itself lacks bone inducibility. Less

目的通过兔尺骨骨缺损模型，研究磷酸三钙/乳酸共聚物（TCP/CPLA）复合人工骨膜的修复效果。TCP是一种常见的骨传导材料，TCP/CPLA复合物易形成膜状结构。方法青年组10只（体重2.5-3.0kg），老年组4只（体重4kg以上）。在双侧尺骨中央造成一个1.5cm的骨缺损，包括骨膜。骨缺损一侧覆盖TCP/CPLA人工骨膜，另一侧覆盖CPLA膜。年轻组术后4周处死2只JWR，8周处死6只，12周处死2只。老龄组于术后8周处死4只家兔。软X射线分析表明， ...更多信息 组织切片用苏木精-伊红染色和Azam染色。结果青年组：术后8周软X线片显示，TCP/CPLA组所有标本缺损两端均完成骨性桥接。术后8周，CPLA组有2例骨桥不完全。组织学上，在TCP/CPLA组中识别到骨形成，但CPLA组中的一个在术后4周显示无骨形成。术后12周，TCP/CPLA组和CPLA组均出现骨桥。Azam染色的组织学显示TCP/CPLA组中的bode形成%，CPLA组中的bode形成%，具有统计学显著性差异。老年组：组织学研究以及软X线图像显示TCP/CPLA组仅1个标本中存在完整的骨桥。结论TCP/CPLA复合人工骨膜治疗骨缺损具有临床应用的可能性。然而，由于TCP/CPLA复合物本身缺乏骨诱导作用，因此这种膜不能预期对老年患者有足够的骨形成。少