权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The Effects of Controlled Tensil Load on the Healing of Reconstructed Ligament

控制拉伸负荷对重建韧带愈合的影响

基本信息

批准号：
06671441
负责人：
MUNETA Takeshi
金额：
$ 1.41万
依托单位：
TOKYO MEDICAL AND DENTAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

This study was performed to investigate the effects of loading history on the healing process of transplanted soft tissue. First, and apparatus which exerts controlled tensile load on the bone-patellar tendon -bone (BTB) specimen in a SD rat model was developed. A cyclic load was given with a cylinder in conjunction with an air compressor. A timer determined the timing of loading by controlling the air compressor. One newton tensile load was given using a TiNi ultra-elastic spring. A frame on which a BTB specimen was installed was put under the skin in the back.A preliminary histologic study indicated fibroblast-like cell invasion into the BTB specimen 4 weeks after transplantation. The effects of frequency of loading was investigated biomechanically 4 weeks after transplantation in this study. The study group consisted of 4 goups : normal, unloaded, 2 times per week and 3 times per day groups. A set of loading was 15 minutes ; one newton loading was given at 1 Hz.Tensile test revealed that the linear stiffiness and maximum failure load of the 3/day loading group were statistically greater than those of the unloaded group. There was no differences of the linear stiffness and maximum failure load between the normal and 3/day loading groups.The results of this study suggested the possibility that higer frequency of loading will prevent deterioration in structual properties of transplanted soft tissue. The apparatus has much potential in the near future that realize detailed investigation of transplanted soft tissue healing.

本研究的目的是调查负荷历史对移植软组织愈合过程的影响。首先，开发了对SD大鼠模型中的骨-髌腱-骨（BTB）样本施加受控拉伸载荷的装置。通过与空气压缩机结合的气缸来施加循环负载。定时器通过控制空气压缩机来确定加载时间。使用 TiNi 超弹性弹簧施加一牛顿拉伸载荷。将安装有 BTB 标本的框架置于背部皮下。初步组织学研究表明，移植后 4 周，成纤维细胞样细胞侵入 BTB 标本中。本研究在移植后 4 周对负载频率的影响进行了生物力学研究。研究组由 4 组组成：正常组、无负载组、每周 2 次组和每天 3 次组。一组加载时间为15分钟；在1 Hz下施加1牛顿载荷。拉伸试验显示，3/天加载组的线性刚度和最大破坏载荷在统计上大于未加载组。正常加载组和每天 3 次加载组之间的线性刚度和最大破坏载荷没有差异。本研究的结果表明，较高的加载频率可能会防止移植软组织结构性能的恶化。该设备在不久的将来具有很大的潜力，可以实现移植软组织愈合的详细研究。