Research of generation of respiratory rhythm

呼吸节律产生的研究

• 批准号: 09671576
• 负责人: INOUE Takuya
• 金额: $ 1.98万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671576/
• 关键词: invertebrate; respiration; central pattern generator; chemoreception; lymnaea stagnalis; central pattern generator

The three neurons that comprise the respiratory central pattern generator in the brain of the snail. Lymnaea stagnalis were examined to elucidate their function. When an hypoxic saline solution was perfused over an isolated brain preparation (i.e., without peripherally nerves), respiratory activity remained unchanged When an hypoxic saline solution was perfused over a semi-intact brain preparation (i.e., peripherally nerves still connected), respiratory activity increased.The Right Peda1 Dorsal I respiratory neuron (RPeD1, that initiates respiratory activity) was then isolated and cultured Perfusion with an hypoxic saline solution produced no change in either the resting membrane or action potentials, suggesting that RPeD1 does not exhibit oxygen chemoreception.To locate and isolate the oxygen chemoreceptor, an attempt was made to separate and culture the neurons of the osphradium ganglion, which lies immediately beneath the pneumostome. All attempts thus far have been unsuccessful.The effects of carbon dioxide on both the isolated and semi-intact brain preparations were also examined by perfusing them with a saline solution equilibrated with a 5% C02/95% 02 gas mixture. Respiratory activity decreased in both preparations.The saline solution used in the experiment above also had a low pH.In an effort to distinguish the effects of pH from the presence of C02, both preparations were perfused with a saline solution equilibrated with 21%02+79%N02 and whose pH had been adjusted with HCl to a pH of 7.0-7.5. Respiratory activity was decreased in both preparations as well.

包括蜗牛大脑中呼吸中心模式发生器的三个神经元。检查lymnaea stagnalis以阐明其功能。当在孤立的脑部制备（即没有周围神经）中灌注低氧盐水溶液时，当在半独立性的大脑制备中灌注低氧盐水溶液时，呼吸活性保持不变（即，周围神经仍然相互连接），呼吸次数增加。用缺氧盐溶液进行培养的灌注不会在静息膜或动作电位上产生任何改变，这表明RPED1不表现出氧化学感受。要定位和隔离氧化学感受器，试图将其分离和培养神经化的神经元的神经元，而这些神经神经节的神经元在pnepneypne ospnepnemosthion的情况下，该神经元与pne蛋白构成了pneyerons of pere the pereomosteromotemolessome。迄今为止，所有尝试都没有成功。二氧化碳对分离的和半独立脑部制剂的影响也通过用盐溶液用5％C02/95％02气体混合物平衡的盐溶液进行灌注。两种制剂中的呼吸活性都降低了。上面实验中使用的盐溶液也有较低的pH值。在努力以区分pH的作用和C02的存在，两种制剂都用与21％02+79％N02平衡的盐溶液进行灌注，并且其pH值已将HCl调节至7.0-7-7.0-7.5.5.5.5。两种制剂的呼吸活性也均降低。