Assessment of ovarian fecundity in terms of telomere length and telomerase activity.

根据端粒长度和端粒酶活性评估卵巢生育力。

• 批准号: 09671662
• 负责人: SAITO Hidekazu
• 金额: $ 0.64万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671662/
• 关键词: Telomere; Telomerase activity; Ovarian fecundity; Granulosa cells; 卵; テロメアーゼ活性

Many studies of telomerase activity has been performed for the clinical application to diagnosis and treatment of cancer. Few studies have been performed about the reproductive cells which have highly proliferative activity. In this study, the relationship between telomerase activity and the development of eggs and embryos was examined.In IVF-ET of human, each follicle was aspirated separately by each syringe and mural granulosa cells and cumulus granulosa cells were obtained from each follicular fluid. Then telomerase activity of both kinds of granulosa cells were measured by TRAP assay. And the telomerase activity of oocyte was measured using mouse oocytes and the fluctuation of the telomerase activity during the developmental stage of oocyte and embryo was examined by TRAP assay.Telomerase activity was detected in both cumulus and mural granulosa cells. And the activity of mural granulosa cells was higher than that of cumulus granulosa cells. Telomerase activity was also present in both oocytes and embryos at various stages. In the oocyte stages, the oocyte 24hrs after PMS injection showed the higher than that of the oocytes 48 hrs after PMS, 4 and 8 hrs after hCG and just after ovulation. In the embryo stages, the telomerase activity was low at one, two and molura stage and the activity started to increase at blastocyst stage.Thus the activity of telomerase is fluctuating during the development of oocyte and embryo, and might have effects of the development of oocyte and embryo.

端粒酶活性在肿瘤诊断和治疗中的临床应用已得到广泛的研究。生殖细胞具有高度增殖活性，对其研究较少。在体外受精-胚胎移植（IVF-ET）过程中，用注射器分别抽吸每个卵泡，从每个卵泡液中分离出壁颗粒细胞和卵丘颗粒细胞，观察端粒酶活性与卵子和胚胎发育的关系。TRAP法检测两种颗粒细胞端粒酶活性。用TRAP法检测了小鼠卵母细胞端粒酶活性的变化，发现卵丘细胞和壁颗粒细胞中均有端粒酶活性表达。壁颗粒细胞的活性高于卵丘颗粒细胞。端粒酶活性也存在于卵母细胞和胚胎在不同时期。在卵母细胞期，注射PMS后24小时的卵母细胞高于PMS后48小时、hCG后4小时和8小时以及排卵后的卵母细胞。在胚胎发育过程中，端粒酶活性在1、2、Molura期较低，囊胚期开始升高，说明端粒酶活性在卵母细胞和胚胎发育过程中是波动的，可能对卵母细胞和胚胎的发育有一定的影响。

项目成果

N.Ohta et al.: "Expression on CD44 in human cumulus and mural granulosa cells of individual patients in in-vitro fertilization programmes"Molecular Human Reproduction. 5. 22-28 (1999)

N.Ohta 等人：“体外受精计划中个体患者的人卵丘和壁颗粒细胞中 CD44 的表达”《分子人类生殖》。

P.Moreira et al.: "Are there any relationship between the fecundity of bilateral ovaries in an individual patient and the incidence of apoptotic granulosa cells?" Human Reproduction. 14. 161-156 (1999)

P.Moreira 等人：“个体患者双侧卵巢的生育力与凋亡颗粒细胞的发生率之间是否存在任何关系？”

P.Moreira et al.: "Are there any relationship between the fecundity of bilateral ovaries in an individual patient and the incidence of apoptotic granulosa cells?"Human Reproduction. 14. 156-161 (1999)

P.Moreira 等人：“个体患者双侧卵巢的生育力与凋亡颗粒细胞的发生率之间是否存在任何关系？”人类生殖。

K.Nakahara et al.: "Ovarian fecundity in patients with endometoriosis can estimated by the incidence of apoptotic bodies"Fertility and Sterility. 69. 931-935 (1998)

K.Nakahara 等人：“子宫内膜异位症患者的卵巢生育能力可以通过凋亡小体的发生率来估计”生育力和不育。

K.Nakahara et al.: "Ovarian fecundity in patients with endometriosis can estimated by the incidence of apoptotic bodies."Fertility and Sterility. 69. 931-935 (1998)

K.Nakahara 等人：“子宫内膜异位症患者的卵巢生育能力可以通过凋亡小体的发生率来估计。”生育力和不育。