Study of factors regulating the development of follicles, oocytes and embryos

卵泡、卵母细胞和胚胎发育调节因素的研究

• 批准号: 12671576
• 负责人: SAITO Hidekazu
• 金额: $ 2.24万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671576/
• 关键词: quality of oocyte; oxidative stress; cell cycle; 8 OhdG; glutathione reductase; 卵; 卵胞; 胚; テロメラーゼ; アポトーシス; 凍結; ヒアルロン酸

Apoptosis, telomerase and oxidative stress are phenomena strongly associated with follicular development and ovulation. In follicles, during natural menstrual cycle, apoptosis occurs in granulosa cells and is correlated with the fate of follicles. We examined apoptosis in granulosa cells during gonadotropin stimulated cycles, and found that, the incidence of apoptotic granulosa cells strongly reflected the quality of oocytes. We also found that telomerase was active in granulosa cells, with mural granulosa cells expressing higher telomerase activity. This seems to be a good indicator of cell proliferation ability.In this study, we found that aging induced apoptosis in granulosa cells, reducing the number of developing follicles and also decreasing the quality of oocytes. Furthermore, the oxidative stress in granulosa cells during ovulation produces 8-OHdG, which reduces the quality of oocytes. While glutathione reductase is also expressed in granulosa cells and oocytes during ovulation. This means the oocytes and granulosa cells also have the mechanisms for eliminating the oxidative stress and maintaining the quality of oocytes. All these facts seem to indicate that these methods might be useful in the evaluation of the quality of oocytes and that further research could help in the improvement of oocyte quality.

细胞凋亡、端粒酶和氧化应激是与卵泡发育和排卵密切相关的现象。在卵泡中，在自然月经周期中，颗粒细胞发生凋亡，并与卵泡的命运相关。我们检测了促性腺激素刺激周期中颗粒细胞的凋亡情况，发现颗粒细胞凋亡的发生率强烈反映了卵母细胞的质量。我们还发现端粒酶在颗粒细胞中有活性，壁粒细胞表达更高的端粒酶活性。这似乎是细胞增殖能力的一个很好的指标。在本研究中，我们发现衰老导致颗粒细胞凋亡，减少发育卵泡的数量，同时降低卵母细胞的质量。此外，排卵期间颗粒细胞的氧化应激会产生8-OHdG，从而降低卵母细胞的质量。而谷胱甘肽还原酶在排卵过程中也在颗粒细胞和卵母细胞中表达。这意味着卵母细胞和颗粒细胞也具有消除氧化应激和维持卵母细胞质量的机制。这些事实似乎表明这些方法在评价卵母细胞质量方面可能是有用的，进一步的研究将有助于提高卵母细胞的质量。

项目成果

Seino T et al.: "Eight-hydroxy-2 1-deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos during an in vitro fertilization and embryo transfer program"Fertil Steril. (in press). (2002)

Seino T 等人：“颗粒细胞中的八羟基-2 1-脱氧鸟苷与体外受精和胚胎移植程序期间卵母细胞和胚胎的质量相关”Fertil Steril。

Saito H,Saito T, 他: "Relatively poor oocyte quality is an indication for intracytoplasmic sperm injection"Fertility & Sterility. 73・3. 465-469 (2000)

Saito H、Saito T 等人：“卵母细胞质量相对较差是胞浆内精子注射的指征”Fertility & Sterility 73・3 (2000)。

Ohta N et al.: "Soluble CD44 in human ovarian follicular fluid"J Assist Reprod Genet. 18. 23-27 (2001)

Ohta N 等人：“人卵巢卵泡液中的可溶性 CD44”J Assist Reprod Genet。

Saito H.et al.: "Hyaluronan in follicular fluids and fertilization of oocytes"Fertil Steril. 74. 1148-1152 (2000)

Saito H.等人：“卵泡液中的透明质酸和卵母细胞的受精”Fertil Steril。

Saito H et al.: "Endometriosis and Oocyte Quality"Gynecol Obstet Invest. 53-suppl1. 46-51 (2002)

Saito H 等人：“子宫内膜异位症和卵母细胞质量”Gynecol Obstet Invest。