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Study of the cholesterol transportation mechanism in embryo

胚胎中胆固醇转运机制的研究

基本信息

批准号：
09671672
负责人：
FURUKAWA Hiroyuki
金额：
$ 1.47万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671672/
关键词：
mouse embryo LDL macrophage implantation コレステロール胚

项目摘要

Changes in cell size of preimplantation mouse embryos and the influences of low density lipoprotein on the development of embryos. The total cell volume (TV) in an embryo, calculated by the diameters of cells, decreased of 29.1% but the total cell surface area (TA) did not change during the first cell division. During 2 to 8 cell stage TV was constant but TA increased of 24-26% after each division. Low density lipoprotein (LDL) in the culture media did not improve the blastocyst rates of embryos cultured from 1 cell stage, but significantly increased the area of trophoblast outgrowth cultured after the early blastocyst stage. However the cell surface area incresae during preimplantation embryogenesis, the development improving effect of LDL were observed only after the blastocyst stage.The concentration and distribution of F4/80-positive macrophages and BM8-positive macro-phages in the uterus of mice during the preimplantation period (between day 1 and 14 of pregnancy) were assessed immunohistochemically. In the endometrium, macrophages were found diffusely on day 0-1 and the number of these cells increased on the lumen side on day 2 and 3.Upon initiation of implantation (day 3-7), significant numbers of macrophages were found on the outside of the decidua formed around the implantation site, but macrophages were not found within the decidua. In the endo-metrium without decidualization, macrophages were found diffusely on day 0-1.Furthermore, this study demonstrates that, during pregnancy, macrophages are not present within the early stage of decidua-lization, but they migrate from a deep layer of the endometrium following the proliferation of the decidua.

着床前小鼠胚胎细胞大小的变化及低密度脂蛋白对胚胎发育的影响。根据细胞直径计算，胚胎中的总细胞体积 (TV) 减少了 29.1%，但在第一次细胞分裂期间，总细胞表面积 (TA) 没有变化。在 2 至 8 细胞阶段，TV 保持不变，但每次分裂后 TA 增加 24-26%。培养基中的低密度脂蛋白（LDL）并没有提高从1细胞阶段开始培养的胚胎的囊胚率，但显着增加了早期囊胚阶段后培养的滋养层生长面积。然而，在着床前胚胎发生过程中细胞表面积增加，LDL对发育的促进作用仅在囊胚期后才观察到。采用免疫组织化学方法评估着床前（妊娠第1天至第14天）小鼠子宫中F4/80阳性巨噬细胞和BM8阳性巨噬细胞的浓度和分布。在子宫内膜中，第0-1天广泛发现巨噬细胞，第2天和第3天，腔侧的细胞数量增加。植入开始后（第3-7天），在植入部位周围形成的蜕膜外部发现大量巨噬细胞，但在蜕膜内未发现巨噬细胞。在未蜕膜化的子宫内膜中，第0-1天就发现了广泛的巨噬细胞。此外，本研究表明，在怀孕期间，巨噬细胞在蜕膜化的早期并不存在，但随着蜕膜的增殖，巨噬细胞从子宫内膜深层迁移出来。