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The relationship between overexpression of cyclin D1 and prognostic factors on pharyngeal cancer. (molecular biological approach)

cyclin D1过表达与咽癌预后因素的关系

基本信息

批准号：
09671759
负责人：
YOSHIDA Tomoyuki
金额：
$ 1.66万
依托单位：
Tokyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671759/
关键词：
cyclin Dl p53 head and neck cancer pharyngeal cancer RT-PCR RT-PCR CyclinDl In situ PCR.

项目摘要

Cyclin D1 is a cell cycle regulator essential for G1-S phase progression. The gene is located on chromosome llq13, which the abnormal amplification has been observed in many tumor cells. It also complexes with many other cell cycle related proteins such as cdk4, PCNA, and makes a cyclin box, which regulates the cell cycle. In this study, we have analyzed cyclin D1 mRNA amplification and protein overexpression in a series of human pharyngeal carcinomas using immunohistochemical and molecular biological methods. The results were analyzed against the clinical and pathological characteristics of the patients.A strong correlation was observed between the cyclin D1 and cdk4 mRNA amplification, suggesting that these genes would be amplified in the same phase in the tumor cell cycles. Also, we found that strong overexpressions of the cyclin D1 protein were seen not only in the poorly differentiated carcinomas, but also in the well-differentiated carcinomas. It suggests that the other genes in this amplicon may also be involved in the progression and differentiation of carcinogenesis. Additionally, we analyzed that the expression of cyclin D1 and cdk4 mRNA amplification had a correlation with the overexpression of PCNA protein. This data are consistent with the notion that the expression of cyclin D1 and cdk4 represents an important role of the cell regulation in pharyngeal carcinomas.

Cyclin D1是G1-S期进程所必需的细胞周期调节因子。该基因位于染色体llq 13上，在许多肿瘤细胞中观察到异常扩增。它还与许多其他细胞周期相关蛋白如cdk 4、PCNA复合，形成一个细胞周期蛋白盒，调节细胞周期。在这项研究中，我们分析了细胞周期蛋白D1的mRNA扩增和蛋白过表达，在一系列人类咽癌用免疫组织化学和分子生物学方法。结果显示，cyclin D1和cdk 4 mRNA的扩增具有较强的相关性，提示这两种基因可能在肿瘤细胞周期的同一时期扩增。此外，我们发现，强烈的过度表达的细胞周期蛋白D1蛋白被认为不仅在低分化癌，但也在高分化癌。提示该扩增子中的其他基因可能也参与了癌的发生、发展和分化。另外，我们还分析了cyclin D1和cdk 4 mRNA扩增与PCNA蛋白过度表达的相关性。这一结果与细胞周期蛋白D1和cdk 4的表达在咽癌细胞调控中起重要作用的观点相一致。