Neurite formation of aging sensory ganglion cells

老化感觉神经节细胞的神经突形成

• 批准号: 09680748
• 负责人: NINOMIYA Takafumi
• 金额: $ 1.28万
• 依托单位: Sapporo Medical University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680748/
• 关键词: Dorsal root ganglion; Neurite formation; Nerve growth factor; NGF receptor; Aging; Cell culture; 神経突起; 偽単極細胞; 神経栄養因子; レセプター

In aging dorsal root ganglion (DRG) cell culture, DRG cells display the multipolar, bipolar or pseudounipolar forms. Some pseudounipolar neurons had many thin neurites elongated from the junction of the stem process or a structure-like swelling body or glomerulus (unipolar 2 cell type). These structures were not seen in fetal or newborn mouse DRG cell cultures. Unipolar2 neurons appeared with an incidence of about 5% in a medium without NTF or supplemented with NT-3, NT-4 or BDNF.However unipolar2 neurons increased about 30% in a medium supplemented with NGF.There is no difference of the number of surviving neurons in each of culture medium. The appearance of this type cell was affected by NGF, not affect other neurotrophic factors. It is suggested that adult DRG neurons remained only the low-affinity NGF-receptor(p75) but not the high-affinity NGF receptors(trk A, B, or C).

在老化的背根神经节（DRG）细胞培养中，DRG 细胞表现出多极、双极或假单极形式。一些假单极神经元有许多细的神经突，从干突的交界处延伸出来，或者有类似结构的肿胀体或肾小球（单极2细胞类型）。这些结构在胎儿或新生小鼠 DRG 细胞培养物中未见。在不含NTF或补充NT-3、NT-4或BDNF的培养基中，单极2神经元的出现率约为5%。然而，在补充NGF的培养基中，单极2神经元增加约30%。每种培养基中存活神经元的数量没有差异。该型细胞的出现受NGF影响，不影响其他神经营养因子。表明成年DRG神经元仅保留低亲和力NGF受体(p75)，而不保留高亲和力NGF受体(trk A、B或C)。

项目成果

Takafumi Ninomiya: "Nerve growth factor affecting the neurite formation of aging mouse dorsal root ganglion cell in vitro." European Journal of Neuroscience. 10. 407 (1998)

Takafumi Ninomiya：“神经生长因子在体外影响衰老小鼠背根神经节细胞的神经突形成。”

Takafumi Ninomiya: "Nerve growth factor affecting the neurite famation of aging mouse dorsal root ganglion cells in vitro" European Journal of Neuroscience. 10. 407 (1998)

Takafumi Ninomiya：“神经生长因子影响体外衰老小鼠背根神经节细胞的神经突形成”《欧洲神经科学杂志》。