STUDY ON RECONSTITUTION OF KERATIN INTERMEDIATE FIBRILS AND PHYSICAL AND BIOCHEMICAL PROPERTIES

角蛋白中原纤维的重构及理化性质研究

• 批准号: 09680857
• 负责人: YAMAUCHI Kiyoshi
• 金额: $ 1.66万
• 依托单位: OSAKA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680857/
• 关键词: KERATIN; PROTEIN REFOLDING; CELL GROWTH; CELL ATTACHMENT; HAIR; WOOL; 細胞増殖性; 繊維; タンパク質; 中間繊維; 生体材料

Keratins, which are the proteins bearing cysteine as much as 10 mole % of total amino acid residues, were extracted from wool and hair as a stable aqueous solution. A solubilization/extraction system was an aqueous solution of urea, 2-mercaptoethanol and a surfactant. Especially, long chain-alkyl sulfate sodium salts and polyoxyethylenedodecylsulfate sodium were effective to enhance the extraction rate. Chief constituents of the keratins ranged from 40000 to 60000 daltons.The keratins in the aqueous solution were refolded into fibrils of about 1-5 micrometers in diameter. The main proteins in the refolded fibrils came from the microfibrils of wool or hair.The keratin films or coatings (on glass) supported the L929 fibroblast cells in the degree better than collagen films or coatings. The cells attached well on the keratin substratum. The results were explained by the polar nature of the keratins and the presence of a RGD-amino acid sequence in the keratin component.

角蛋白是一种含有半胱氨酸的蛋白质，占总氨基酸残基的 10 摩尔%，以稳定的水溶液形式从羊毛和头发中提取。溶解/萃取系统是尿素、2-巯基乙醇和表面活性剂的水溶液。特别是长链烷基硫酸钠和聚氧乙烯十二烷基硫酸钠对于提高提取率是有效的。角蛋白的主要成分范围为40000至60000道尔顿。水溶液中的角蛋白重新折叠成直径约1-5微米的原纤维。重折叠原纤维中的主要蛋白质来自羊毛或头发的微原纤维。角蛋白膜或涂层（在玻璃上）比胶原膜或涂层更好地支持L929成纤维细胞。细胞很好地附着在角蛋白基质上。该结果可以通过角蛋白的极性性质和角蛋白成分中 RGD 氨基酸序列的存在来解释。

项目成果

K.YAMAUCHI: "POLYMERS FOR TISSUE ENGINEERING" VSP BV.UTRECHT. 329-340 (1998)

K.YAMAUCHI：“组织工程聚合物”VSP BV.UTRECHT。

K.YAMAUCHI, AND A.KHODA: "NOVEL PROTEINOUS MICROCAPSULES FROM WOOL KERATINS" COLLOIDS AND SURFACES BIOINTERFACES B. 10. 35-39 (1997)

K.YAMAUCHI 和 A.KHODA：“来自羊毛角蛋白的新型蛋白质微胶囊”胶体和表面生物界面 B.10.35-39 (1997)

K.Yamauchi, A.Khoda: "Novel Proteinous Microcapsules from Wool Keratins" Colloids and Surfaces,Biointerfaces B. 9. 117-119 (1997)

K.Yamauchi、A.Khoda：“来自羊毛角蛋白的新型蛋白质微胶囊”胶体和表面，生物界面 B. 9. 117-119 (1997)

K.Yamauchi,M.Maniwa,T.Mori: "Cultivation of Fibroblast Cells on Keratin-coated Substrate" J.Biomat.Sci.Polymer Edition's Special Issue. 9. 259-270 (1998)

K.Yamauchi、M.Maniwa、T.Mori：“在角蛋白涂层基质上培养成纤维细胞”J.Biomat.Sci.Polymer Edition 的特刊。

K.Yamauchi,M.Maniwa,T.Mori: "Cultivation of Fibroblast Cells on Keratin-coated Substrate" J.Biomat.Sci.Polymer Edition's Special lssue. 9. 259-270 (1998)

K.Yamauchi、M.Maniwa、T.Mori：“在角蛋白涂层基质上培养成纤维细胞”J.Biomat.Sci.Polymer Edition 的特刊。