STUDIES ON SOME FACTORS RESPOSIBLE FOR CONJUGATED LINOLEIC ACID CONTENT IN FOODS OF RUMINANT ORIGINS

反刍动物食品中共轭亚油酸含量影响因素的研究

基本信息

批准号：
13660269
负责人：
YAMAUCHI Kiyoshi
金额：
$ 2.37万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660269/
关键词：
Conjugated linoleic acid (CLA)c9, t11 isomer of CLA Foods of ruminant origins Concentrate Roughage ration Precursor of c9, t11 isomer Rumen liquor Linoleic acid α -Linolenic acid 反芻家畜出来食品ルーメン内微生物叢共役リノール酸 cis9, trans11 CLA生成飼料

项目摘要

Conjugated linoleic acid (CLA) is a collective term used to describe one or more positional and geometric isomers of linoleic acid. Of the CLA isomers, cis9, trans11 isomer which is found primarily in ruminants amounts as much as 80〜90% of the total CLA content of foods of ruminant origins. The cis9, trans11 isomer of CLA has been shown in animals to have an anticarcinogenic property. It is believed that cis9, trans11 CLA isomer is produced in the rumen as intermediates in the biohydrogenation of dietary linoleic acid to stearic acid. Our works have demonstrated that the cis9, trans11 CLA contents of meat and fat from ruminants fed a relatively high roughage ration is significantly higher than those from ruminants fed a relatively high concentrate ration, with the results suggesting that α -linolenic acid present predominantly in pasture lipid might be involved in the formation of cis9, trans11 CLA.Therefore, crashed corn high in linoleic acid and Italian ryegrass hay high in α -linolenic acid were incubated with rumen liquor culture. The yield of cis9, trans11 CLA from the Italian ryegrass was siginificantly higher than that from the crashed corn. This findings suggested the formation of cis9, trans11 CLA via α -linolenic acid. α -Linolenate was incubated with rumen liquor culture. The formation of cis9, trans 11 CLA from α -linolenate was first observed in the rumen liquor culture, and the pH value of the rumen liquor culture also influenced the metabolism. The results showed that α -linolenate incubated with rumen liquor culture produced three intermediate metabolites of which c9, t11, c15 octadecatrienoic acid might be involved in the formation of cis9, trans11 CLA in the rumen. The results obtained in the present study confirmed for the first time that in additoin to linoleic acid, α -linolenic acid present predominantly in pasture lipid may serve as a potential precursor of cis9, trans 11 CLA in the rumen.

共轭亚油酸（CLA）是一个用于描述亚油酸的位置和几何异构体的集体术语。在CIS9，Trans11异构体中，反刍动物的主要量高达80-90％，占反刍动物起源食物的总CLA含量。 CIS9，CLA的Trans11异构体已显示在动物中具有抗癌特性。据信，在瘤胃中，瘤胃中产生了CIS9，Trans11 CLA异构体，作为中间体在饮食中的亚油酸生物氢化与偷酸中的生物氢化。 Our works have demonstrated that the cis9, trans11 CLA contents of meat and fat from ruminants fed a relatively high roughage ration is significantly higher than those from ruminants fed a relatively high concentration ration, with the results suggesting that α -linolenic acid present predominantly in pasture lipid might be involved in the formation of cis9, trans11 CLA.Therefore, crashed corn high in linoleic acid and Italian ryegrass将高中α-纤维烯酸中的干草与瘤胃液培养一起孵育。来自意大利黑麦草的trans11 cla的产量高于崩溃的玉米。这一发现表明，通过α-氯苯酸形成顺式，Trans11 CLA。 α-纤维苯甲酸酯与瘤胃液体培养物孵育。首先在瘤胃液体培养物中观察到来自α-氯化酸盐的顺式，Trans11 CLA的形成，瘤胃液体培养物的pH值也影响了代谢。结果表明，与瘤胃液体培养的α-氯化酸盐产生了三种中间代谢产物，其中C9，T11，C15八环酸酸可能参与CIS9，Trans11 CLA的形成。在本研究中获得的结果首次证实，在对亚油酸的添加剂中，主要在牧场脂质中主要存在的α-烯丙基酸可能是CIS9的潜在前体，在瘤胃中trans 11 CLA。