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STUDIES ON SOME FACTORS RESPOSIBLE FOR CONJUGATED LINOLEIC ACID CONTENT IN FOODS OF RUMINANT ORIGINS

反刍动物食品中共轭亚油酸含量影响因素的研究

基本信息

批准号：
13660269
负责人：
YAMAUCHI Kiyoshi
金额：
$ 2.37万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660269/
关键词：
Conjugated linoleic acid (CLA)c9, t11 isomer of CLA Foods of ruminant origins Concentrate Roughage ration Precursor of c9, t11 isomer Rumen liquor Linoleic acid α -Linolenic acid 反芻家畜出来食品ルーメン内微生物叢共役リノール酸 cis9, trans11 CLA生成飼料

项目摘要

Conjugated linoleic acid (CLA) is a collective term used to describe one or more positional and geometric isomers of linoleic acid. Of the CLA isomers, cis9, trans11 isomer which is found primarily in ruminants amounts as much as 80〜90% of the total CLA content of foods of ruminant origins. The cis9, trans11 isomer of CLA has been shown in animals to have an anticarcinogenic property. It is believed that cis9, trans11 CLA isomer is produced in the rumen as intermediates in the biohydrogenation of dietary linoleic acid to stearic acid. Our works have demonstrated that the cis9, trans11 CLA contents of meat and fat from ruminants fed a relatively high roughage ration is significantly higher than those from ruminants fed a relatively high concentrate ration, with the results suggesting that α -linolenic acid present predominantly in pasture lipid might be involved in the formation of cis9, trans11 CLA.Therefore, crashed corn high in linoleic acid and Italian ryegrass hay high in α -linolenic acid were incubated with rumen liquor culture. The yield of cis9, trans11 CLA from the Italian ryegrass was siginificantly higher than that from the crashed corn. This findings suggested the formation of cis9, trans11 CLA via α -linolenic acid. α -Linolenate was incubated with rumen liquor culture. The formation of cis9, trans 11 CLA from α -linolenate was first observed in the rumen liquor culture, and the pH value of the rumen liquor culture also influenced the metabolism. The results showed that α -linolenate incubated with rumen liquor culture produced three intermediate metabolites of which c9, t11, c15 octadecatrienoic acid might be involved in the formation of cis9, trans11 CLA in the rumen. The results obtained in the present study confirmed for the first time that in additoin to linoleic acid, α -linolenic acid present predominantly in pasture lipid may serve as a potential precursor of cis9, trans 11 CLA in the rumen.

共轭亚油酸（CLA）是用于描述亚油酸的一种或多种位置和几何异构体的统称。在CLA异构体中，主要存在于反刍动物体内的顺式9、反式11异构体占反刍动物源性食物中总CLA含量的80 ~ 90%。CLA的顺式9、反式11异构体已在动物实验中被证明具有抗癌特性。研究认为，在饲料亚油酸生物氢化为硬脂酸的过程中，瘤胃产生了顺式、反式CLA异构体作为中间产物。我们的研究表明，饲喂较高粗料日粮的反刍动物的肉和脂肪中顺式9、反式11 CLA的含量显著高于饲喂较高精料日粮的反刍动物，这表明牧场脂肪中主要存在的α -亚麻酸可能参与了顺式9、反式11 CLA的形成。因此，采用瘤胃白酒培养法培养高亚油酸玉米和高α -亚麻酸意大利黑麦草干草。意大利黑麦草中cis9、trans11 CLA的产量显著高于玉米。这表明顺式、反式CLA是通过α -亚麻酸形成的。用瘤胃白酒培养α -亚麻酸盐。α -亚麻酸盐在瘤胃白酒培养中首次形成顺式9、反式11 CLA，瘤胃白酒培养的pH值也影响其代谢。结果表明，α -亚麻酸在瘤胃白酒培养中产生了3种中间代谢物，其中c9、t11、c15十八碳三烯酸可能参与了瘤胃中顺式9、反式11 CLA的形成。本研究结果首次证实，除亚油酸外，牧草脂肪中主要存在的α -亚麻酸可能是瘤胃中顺式、反式CLA的潜在前体。