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STUDIES ON SOME FACTORS RESPOSIBLE FOR CONJUGATED LINOLEIC ACID CONTENT IN FOODS OF RUMINANT ORIGINS

反刍动物食品中共轭亚油酸含量影响因素的研究

基本信息

批准号：
13660269
负责人：
YAMAUCHI Kiyoshi
金额：
$ 2.37万
依托单位：
University of Miyazaki
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660269/
关键词：
Conjugated linoleic acid (CLA)c9, t11 isomer of CLA Foods of ruminant origins Concentrate Roughage ration Precursor of c9, t11 isomer Rumen liquor Linoleic acid α -Linolenic acid 反芻家畜出来食品ルーメン内微生物叢共役リノール酸 cis9, trans11 CLA生成飼料

项目摘要

Conjugated linoleic acid (CLA) is a collective term used to describe one or more positional and geometric isomers of linoleic acid. Of the CLA isomers, cis9, trans11 isomer which is found primarily in ruminants amounts as much as 80〜90% of the total CLA content of foods of ruminant origins. The cis9, trans11 isomer of CLA has been shown in animals to have an anticarcinogenic property. It is believed that cis9, trans11 CLA isomer is produced in the rumen as intermediates in the biohydrogenation of dietary linoleic acid to stearic acid. Our works have demonstrated that the cis9, trans11 CLA contents of meat and fat from ruminants fed a relatively high roughage ration is significantly higher than those from ruminants fed a relatively high concentrate ration, with the results suggesting that α -linolenic acid present predominantly in pasture lipid might be involved in the formation of cis9, trans11 CLA.Therefore, crashed corn high in linoleic acid and Italian ryegrass hay high in α -linolenic acid were incubated with rumen liquor culture. The yield of cis9, trans11 CLA from the Italian ryegrass was siginificantly higher than that from the crashed corn. This findings suggested the formation of cis9, trans11 CLA via α -linolenic acid. α -Linolenate was incubated with rumen liquor culture. The formation of cis9, trans 11 CLA from α -linolenate was first observed in the rumen liquor culture, and the pH value of the rumen liquor culture also influenced the metabolism. The results showed that α -linolenate incubated with rumen liquor culture produced three intermediate metabolites of which c9, t11, c15 octadecatrienoic acid might be involved in the formation of cis9, trans11 CLA in the rumen. The results obtained in the present study confirmed for the first time that in additoin to linoleic acid, α -linolenic acid present predominantly in pasture lipid may serve as a potential precursor of cis9, trans 11 CLA in the rumen.

共轭亚油酸（CLA）是用于描述亚油酸的一种或多种位置异构体和几何异构体的统称。在CLA异构体中，主要存在于反刍动物中的顺式9、反式11异构体的含量高达反刍动物来源食品中CLA总含量的80〜90%。 CLA 的 cis9、trans11 异构体已在动物身上显示出具有抗癌特性。据信，cis9、trans11 CLA异构体是在瘤胃中产生的，作为膳食亚油酸生物氢化为硬脂酸的中间体。我们的研究表明，饲喂较高粗饲料的反刍动物的肉和脂肪中cis9、trans11 CLA含量显着高于饲喂较高精饲料的反刍动物，结果表明主要存在于牧草油脂中的α-亚麻酸可能参与了cis9、trans11 CLA的形成。因此，粉碎玉米和意大利黑麦草的亚油酸含量较高。将富含α-亚麻酸的干草与瘤胃液体培养物一起孵育。意大利黑麦草的 cis9、trans11 CLA 产量明显高于碎玉米。这一发现表明顺式9、反式11 CLA 通过α-亚麻酸形成。 α-亚麻酸酯与瘤胃液体培养物一起孵育。 α-亚麻酸形成顺式9、反式11CLA首先是在瘤胃液体培养物中观察到的，并且瘤胃液体培养物的pH值也影响代谢。结果表明，α-亚麻酸与瘤胃液培养物一起孵育产生3种中间代谢产物，其中c9、t11、c15十八碳三烯酸可能参与瘤胃中cis9、trans11 CLA的形成。本研究的结果首次证实，除了亚油酸之外，主要存在于牧草脂质中的α-亚麻酸可能是瘤胃中cis9、trans 11 CLA的潜在前体。