The fermentative ability of primate gut microbiome under "humanization"

“人性化”下灵长类肠道微生物组的发酵能力

• 批准号: 21J15166
• 负责人: LEE WAN YI
• 金额: $ 0.96万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-28 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21J15166/
• 关键词: gut microbiome; great ape; fermentative ability; primate feeding ecology; gut microbiom; phylosymbiosis

This project aims to examine and compare the fermentative ability of gut microbiome harbored by the great apes and humans. Through comparison, this project explores the relative importance of phylogeny and diet in shaping hominoid's fermentative ability and gut microbiome.The main achievement of this year is the field trips to Sabah Malaysia - the natural habitat of orangutan Pongo pygmaeus. During the trip, I successfully tracked 11 orangutan individuals to collect their fresh feces and conducted the in vitro fermentation assay. The results of in vitro fermentation assay were, however, opposite to our expectation - there was no significant difference in the fermentative ability between the captive and wild orangutans. I will conduct DNA experiment to determine the gut microbiota.In addition to that, I collected fecal samples from three sympatric primate species (long-tailed macaques, red leaf monkey and pig-tailed macaques) to understand how sharing of habitat/diet may affect the gut microbiome of non-human primates.

该项目旨在检查和比较类人猿和人类肠道微生物组的发酵能力。通过比较，本项目探讨了发育和饮食在塑造类人猿的发酵能力和肠道微生物组方面的相对重要性。今年的主要成果是实地考察了马来西亚沙巴-猩猩Pongo pygmaeus的自然栖息地。在旅途中，我成功地跟踪了11只猩猩，收集了它们的新鲜粪便，并进行了体外发酵试验。然而，体外发酵试验的结果与我们的预期相反-圈养和野生猩猩之间的发酵能力没有显着差异。我将进行DNA实验，以确定肠道菌群。此外，我还收集了三种同域灵长类动物（长尾猕猴，红叶猴和猪尾猕猴）的粪便样本，以了解栖息地/饮食的共享如何影响非人类灵长类动物的肠道菌群。

项目成果

Exploring factors governing the gut microbiome of Japanese macaques

探索控制日本猕猴肠道微生物组的因素

• 发表时间: 2022
• 作者: A. Sawada;T. Hayakawa;Y. Kurihara;W. Lee;G. Hanya;Wanyi Lee
• 通讯作者: Wanyi Lee

Diet-related factors showed greater effects than host phylogeny in shaping the gut microbiota of Japanese macaques

饮食相关因素对日本猕猴肠道微生物群的塑造比宿主系统发育的影响更大

• 发表时间: 2022
• 作者: 西田 紘士;今見 考志;石濱 泰;Wanyi Lee
• 通讯作者: Wanyi Lee

In vitro digestion and fermentation of Japanese macaque ( <i>Macaca fuscata</i> ) food: The influence of food type and particle size

日本猕猴（<i>Macaca fuscata</i>）食物的体外消化和发酵：食物类型和颗粒大小的影响

• DOI: 10.1002/ajp.23470
• 发表时间: 2023
• 期刊: American Journal of Primatology
• 影响因子: 2.4
• 作者: He Tianmeng;Lee Wanyi;Hanya Goro
• 通讯作者: Hanya Goro

Seasonal variation in the fermentative ability of Japanese macaques' gut microbiome

日本猕猴肠道微生物群发酵能力的季节性变化

• 发表时间: 2022
• 作者: Lee Wanyi;He Tianmeng;Yosuke Kurihara;Izumi Shiroishi;Sayaka Tsuchida;Kazunari Ushida;Hanya Goro
• 通讯作者: Hanya Goro