Novel siderophore reductases in Gram-negative bacteria

革兰氏阴性细菌中的新型铁载体还原酶

• 批准号: 520193605
• 负责人: Professor Dr. Henning Tidow
• 金额: --
• 依托单位: Institut für Biochemie und Molekularbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/520193605?language=en
• 关键词: Novel; siderophore; reductases; Gram; negative

Ferric-siderophore pathways are not only a way for bacteria and fungi to acquire the essential ion Fe3+ but also offer a possibility for the development of novel antimicrobial compounds. Our ultimate long-term goal is to understand the molecular mechanisms of Fe3+/siderophore reduction for various protein families from Gram-negative pathogens and to subsequently use this knowledge to explore the design of novel siderophore-antibiotic conjugates. Within this project, we focus on the reduction mechanism of inner membrane reductases of the FoxB (COG3182) family. We seek to understand how membrane reductases of the FoxB family from Gram-negative pathogens function to release ferrous iron from their siderophore substrates. In addition, we will identify the electron donor for the Fe-siderophore reduction and determine the complex structure with FoxB.

铁-铁载体途径不仅是细菌和真菌获得必需离子Fe 3+的途径，而且为开发新型抗菌化合物提供了可能。我们最终的长期目标是了解来自革兰氏阴性病原体的各种蛋白质家族的Fe 3 +/铁载体还原的分子机制，并随后使用这些知识来探索新型铁载体-抗生素缀合物的设计。在本项目中，我们重点研究FoxB（COG 3182）家族内膜还原酶的还原机制。我们试图了解如何从革兰氏阴性病原体的FoxB家族的膜还原酶的功能，以释放亚铁从其铁载体基板。此外，我们将确定的Fe-铁载体还原的电子供体，并确定与FoxB的复杂结构。