Novel siderophore reductases in Gram-negative bacteria

铁-铁载体途径不仅是细菌和真菌获取必需离子Fe3+的途径，而且为开发新型抗菌化合物提供了可能。我们最终的长期目标是了解革兰氏阴性病原体中Fe3+/铁载体还原各种蛋白质家族的分子机制，并随后利用这些知识探索新型铁载体-抗生素偶联物的设计。在本项目中，我们重点研究FoxB （COG3182）家族的内膜还原酶的还原机制。我们试图了解革兰氏阴性病原体FoxB家族的膜还原酶如何从它们的铁载体底物中释放亚铁。此外，我们将确定铁铁基团还原的电子给体，并确定与FoxB的配合结构。

Ferric-siderophore pathways are not only a way for bacteria and fungi to acquire the essential ion Fe3+ but also offer a possibility for the development of novel antimicrobial compounds. Our ultimate long-term goal is to understand the molecular mechanisms of Fe3+/siderophore reduction for various protein families from Gram-negative pathogens and to subsequently use this knowledge to explore the design of novel siderophore-antibiotic conjugates. Within this project, we focus on the reduction mechanism of inner membrane reductases of the FoxB (COG3182) family. We seek to understand how membrane reductases of the FoxB family from Gram-negative pathogens function to release ferrous iron from their siderophore substrates. In addition, we will identify the electron donor for the Fe-siderophore reduction and determine the complex structure with FoxB.