Cell cycle-dependent transcription of cyclin B2

细胞周期蛋白B是细胞分裂周期进程的重要调节因子。它与cdc2蛋白激酶结合形成促成熟因子。MPF复合物是细胞进行有丝分裂所必需的。B型细胞周期蛋白在哺乳动物中有两种亚型，细胞周期蛋白B1和B2。这些蛋白质出现在S期，并进一步积累到G2和有丝分裂，然后在从中期到后期的过渡中消失。产生振荡的一个先决条件是在转录水平上控制表达。我们已经确定了负责细胞周期依赖性转录的DNA元件，从细胞周期蛋白B2启动子。本研究项目旨在鉴定与这些元件上的DNA接触的转录因子。实验的另一个方面是了解DNA结合蛋白的调节。因此，我们将研究视网膜母细胞瘤肿瘤抑制蛋白家族的成员是否在这种调节中发挥任何作用。

Cyclin B is an important regulator for the progression through the cell division cycle. It associates with the cdc2 protein kinase to form the maturation-promoting factor. The MPF complex is necessary for cells to undergo mitosis. B-type cyclins exist in two isoforms in mammalian organisms, cyclin B1 and B2. These proteins appear in S-phase and accumulate further into G2 and mitosis before disappearing at transition from metaphase to anaphase. One prerequisite for the resulting oscillations is control of expression on the transcriptional level. We have identified the DNA elements which are responsible for cell cycle-dependent transcription from the cyclin B2 promoter. This research project aims at identifying the transcription factors contacting the DNA at these elements. Another aspect of the experiments is to understand the regulation of the DNA binding proteins. Therefore we will study if members of the retinoblastoma tumour suppressor protein family have any role in this regulation.