Structural study of muscle contraction and photochemical cycle of purple membrane by using synchrotron radiation

同步辐射对肌肉收缩和紫膜光化学循环的结构研究

• 批准号: 59380027
• 负责人: MITSUI Toshio
• 金额: $ 8.06万
• 依托单位: Faculty of Engineering Science, Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59380027/
• 关键词: Muscle; Actomyosin; Molecular Mechanism of Muscle Contraction; Purple Membrane; Bateriorhodopsin; Synchrotron Radiation; Dynamical Structure Analysis; 2次元X線検出器

1. Molecular mechanism of muscle contraction(1) Time-resolved diffraction studies were done on muscle contraction by using synchrotron radiation. Especially, changes of diffraction patterns were studied in detail for isometrically contracting muscle during stretch, release and sinusoidal length changes. (Matsubara, Sugi, Tanaka, K. Wakabayashi, Amemiya, Mitsui) Obtained data seem to be very helpful to discuss the motion of myosin heads during muscle contraction.(2) Structure of skeletal muscle of rabbit was quite different at 5゜C from that at 25゜C. (T. Wakabayashi)(3) Single-headed myosin can produce tension. (Yanagida)(4) In muscle filaments, the elastic component exists mainly in myosin head. (Tawada)(5) Superprecipitation was studied in connection with ATPase activity by using rutin. (Ebashi)(6) Chemical processes in muscle contraction were studied by time-resolved thermal measurements. (Kodama)(7) A self-induced translation model for myosin motion was proposed to explain recent experimental data. (Mitsui)2. Photochemical cycle of the purple membrane of Halobacterium halobium(1) Diffraction studies were made on bacteriorhodopsin by a heavy-atom replacement method. (Tokunaga)(2) Location of retinal and mechanism of <H^+> pumping were discussed upon spectroscopic and diffraction data. (Ikegami)(3) The intermediate state of bacteriorhodopsin in the photochemical cycle was studied. (Hamanaka, Kito)3. Instrumentation(1) Two-dimensional PSPC was improved to get good time resolution. (Tanaka, Hashizume)(2) Recently invented imaging plate was found to be very useful for muscle studies. (Amemiya, K. Wakabayashi)(3) Low temperaure cell was made to study photochemical cycle of the purple membrane. (Kataoka)

1.肌肉收缩的分子机制(1)利用同步辐射对肌肉收缩进行了时间分辨衍射研究。重点研究了等长收缩肌肉在拉伸、释放和正弦长度变化过程中衍射图的变化。(2)兔骨骼肌在5゜C和25゜C时的结构有很大不同。(3)单头肌球蛋白能产生张力。在肌丝中，弹性成分主要存在于肌球蛋白头部。(Tawada)(5)用芦丁研究了超沉淀与ATPase活性的关系。(Ebashi)(6)用时间分辨热测量方法研究了肌肉收缩的化学过程。(小玉)(7)提出了肌球蛋白运动的自诱导平移模型来解释最近的实验数据。(Mitsui)2.盐生盐杆菌紫膜的光化学循环(1)用重原子置换法对细菌视紫红质进行了衍射研究。(Tokunaga)(2)根据光谱和衍射数据讨论了视网膜的位置和~lt；H^+&gt；泵浦机制。(3)研究了细菌视紫红质在光化学循环中的中间态。(Hamanaka，Kito)3.仪器(1)改进了二维PSPC，获得了良好的时间分辨率。(田中，Hashizume)(2)最近发明的成像板被发现对肌肉研究非常有用。(3)制作低温池，研究紫膜的光化学循环。(片冈)

项目成果

Chaen,S.: J.Biol.Chem.261. 613632-1363 (1986)

Chaen，S.：J.Biol.Chem.261。

wakabayashi,K.: Biophys.J.47. 847-850 (1985)

若林，K.：生物物理学.J.47。

Matsubara,I.: J.Physiol.,Lond.361. 151-163 (1985)

松原，I.：J.Physiol.，Lond.361。

Takeda,K.: Biosci.Rep.5. 615-623 (1985)

武田，K.：Biosci.Rep.5。

Yoshiyuki Amemiya: "Laser-stimulated luminescence used to measure X-ray diffraction of a contracting striated muscle" Science. in press (1987)

Yoshiyuki Amemiya：“激光刺激发光用于测量收缩横纹肌的 X 射线衍射”《科学》。