Studies on the non-specific immunomodulator effects in animals by treatment of Toxoplasma lysate antigens
弓形虫裂解物抗原治疗动物非特异性免疫调节作用的研究
基本信息
- 批准号:59440017
- 负责人:
- 金额:$ 16.32万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (A)
- 财政年份:1984
- 资助国家:日本
- 起止时间:1984 至 1986
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
When animals (mice, rats and cattle) were pretreated with Toxoplasma lysate antigens(TLA), lymphokines such as IFN,MAF,MIF,TOXO-GIF appeared in their blood and they showed a strong non-specific resistance. In consequence, in order to clarify the defence mechanisms which can be induced by TLA against various infections, the mass cultivation of Toxoplasma organisms is necessary. Within the research period of this Grant, we established as the lst report in the world, a method of mass and long term propagation of Toxoplasma in suspension cultures for 30-60 days period and also found a technique for separation of the organisms from host cells automatically, using a microcomputer-controlled system. Then, TLA was used to examine the effect of immunomodulator in reducing clinical symptoms of Babesia, Plasmodium or Theileria infection in animals. In the main results obtained in our studies, non of the mice in the non-TLA treated control groups survived for more than 2 weeks after Babesia or Plasmodium infection, however, mice pretreated with TLA alone or together with lymphokiens or Obioactin survived in a high percentage. In the pretreated cattle infected with Theileria, the clinical symptoms and anemia were extremly slight as compared with those of the non-TLA treated cattle. When animals were treated with TLA, Thy-1 and <Lyt^1> positive and <Lyt^2> negative lymphocytes were found to accumulate remarkably in the thymus and spleen and there was a remarkable increase in the immune response of the host to infection with protozoan organisms.
用弓形虫裂解抗原(TLA)预处理动物(小鼠、大鼠和牛)后,其血液中出现IFN、MAF、MIF、TOXO-GIF等淋巴因子,并表现出较强的非特异性抵抗。因此,为了阐明TLA可以诱导的针对各种感染的防御机制,弓形虫生物体的大量培养是必要的。在本基金的研究期间,我们建立了世界上第一个弓形虫在悬浮培养中大量长期繁殖30-60天的方法,并建立了一种利用微机控制系统自动分离弓形虫和宿主细胞的技术。然后,使用TLA检查免疫调节剂在减轻动物巴氏杆菌、疟原虫或泰勒虫感染的临床症状中的作用。在我们的研究中获得的主要结果中,在非TLA处理的对照组中没有小鼠在巴氏疟原虫或疟原虫感染后存活超过2周,然而,单独用TLA或与淋巴因子或Obioactin一起预处理的小鼠以高百分比存活。与未用TLA处理的牛相比,在感染泰勒虫的预处理牛中,临床症状和贫血极其轻微。当动物用TLA处理时,发现Thy-1和<Lyt^1>阳性和<Lyt^2>阴性淋巴细胞在胸腺和脾脏中显著积聚,并且宿主对原生动物生物体感染的免疫应答显著增加。
项目成果
期刊论文数量(17)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
鈴木直義著,伊沢久夫,清水悠紀臣編: "獣医学 1986:オビオアクチン-OBIOACTIN-生体応答修飾物質(BRM)中の天然免疫調整剤として" 近代出版, (1986)
Naoyoshi Suzuki、Hisao Izawa、Yukiomi Shimizu(编辑):“兽医学 1986:Obioactin - 作为生物反应调节剂 (BRM) 中的天然免疫调节剂”Kindai Publishing,(1986)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
SATO,M.;HORI,Z.;HIROSE,T.;SUZUKI,N.: "Immune effect of Toxoplasma lysate antigen on cattle against Theileria sergenti infection" Jpn. J. Vet. Sci.47. 921-929 (1985)
SATO,M.;HORI,Z.;HIROSE,T.;SUZUKI,N.:“弓形虫裂解物抗原对牛对抗谢氏泰勒虫感染的免疫作用”Jpn。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
SUZUKI Naoyoshi (Edited by Minami,T.; Fujinaga,T.): Kindai Shuppan Co. Lts., Tokyo. Haemomicrobiological Diseases in Veterinary Medicine ( in Japanese ), 386 (1986)
铃木直吉(Minami,T. 编辑;Fujinaga,T.):Kindai Shuppan Co. Lts.,东京。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
OGAWA,R.;SATO,M.;HIROSE,T.;SUZUKI,N.: Jpn.J.Vet.Sci.47. 225-236 (1985)
小川,R.;佐藤,M.;广濑,T.;铃木,N.:Jpn.J.Vet.Sci.47。
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SUZUKI Naoyoshi其他文献
SUZUKI Naoyoshi的其他文献
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{{ truncateString('SUZUKI Naoyoshi', 18)}}的其他基金
Developing a Learning Support System for Remote Body Movement Instruction : An Application in Physical Assessment Skill Training
开发远程身体运动指导学习支持系统:在体能评估技能训练中的应用
- 批准号:
20300270 - 财政年份:2008
- 资助金额:
$ 16.32万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on newly synthesized peptide as an immunoregulator against protozoan infection
新合成肽作为抗原虫感染免疫调节剂的研究
- 批准号:
06556053 - 财政年份:1994
- 资助金额:
$ 16.32万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular Immunological Studies on The Prevention and Diagnoses of Protozoan Infection
原虫感染预防和诊断的分子免疫学研究
- 批准号:
03556041 - 财政年份:1991
- 资助金额:
$ 16.32万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Studies on no-specifical immunoregulatory effects to the host by the addministration of newly activated-peptides derived from Toxoplasma lysate substance
弓形虫裂解物衍生的新激活肽对宿主的非特异性免疫调节作用的研究
- 批准号:
03404013 - 财政年份:1991
- 资助金额:
$ 16.32万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
Immunopotentiatroy Effects of TLA and Obioactin on the Acquired Immunodeficiency Syndroms (AIDS) in animals
TLA 和 Obioactin 对动物获得性免疫缺陷综合症 (AIDS) 的免疫增强作用
- 批准号:
62440020 - 财政年份:1987
- 资助金额:
$ 16.32万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
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