Studies on the non-specific immunomodulator effects in animals by treatment of Toxoplasma lysate antigens

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When animals (mice, rats and cattle) were pretreated with Toxoplasma lysate antigens(TLA), lymphokines such as IFN,MAF,MIF,TOXO-GIF appeared in their blood and they showed a strong non-specific resistance. In consequence, in order to clarify the defence mechanisms which can be induced by TLA against various infections, the mass cultivation of Toxoplasma organisms is necessary. Within the research period of this Grant, we established as the lst report in the world, a method of mass and long term propagation of Toxoplasma in suspension cultures for 30-60 days period and also found a technique for separation of the organisms from host cells automatically, using a microcomputer-controlled system. Then, TLA was used to examine the effect of immunomodulator in reducing clinical symptoms of Babesia, Plasmodium or Theileria infection in animals. In the main results obtained in our studies, non of the mice in the non-TLA treated control groups survived for more than 2 weeks after Babesia or Plasmodium infection, however, mice pretreated with TLA alone or together with lymphokiens or Obioactin survived in a high percentage. In the pretreated cattle infected with Theileria, the clinical symptoms and anemia were extremly slight as compared with those of the non-TLA treated cattle. When animals were treated with TLA, Thy-1 and <Lyt^1> positive and <Lyt^2> negative lymphocytes were found to accumulate remarkably in the thymus and spleen and there was a remarkable increase in the immune response of the host to infection with protozoan organisms.