Immunopotentiatroy Effects of TLA and Obioactin on the Acquired Immunodeficiency Syndroms (AIDS) in animals
TLA 和 Obioactin 对动物获得性免疫缺陷综合症 (AIDS) 的免疫增强作用
基本信息
- 批准号:62440020
- 负责人:
- 金额:$ 17.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (A)
- 财政年份:1987
- 资助国家:日本
- 起止时间:1987 至 1990
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Toxoplasma gondii has been reported to stimulate non-specific resistance to rodent malaria and babesia diseases. Mice that were treated systemically with Toxoplasma lysate antigen (TLA) were highly resistant to infection with Plasmodium berghei and Babesia rodhaini. Consequently, studies of the behavior of lymphocytes and natural killer (NK) cells and lymphokines activated killer (LAK) cells from TLA sensitized animals may clarify the factors responsible for resistance to infection with protozoan parasites and with tumors.When adult BALB/C mice were sensitized with two intramuscular injection of TLA at 2 week interval, the numbers of Sig(+), Thy-1(+), Lyt-2, 2(+), and asialo GM1(+) cells in the spleen, liver and peripheral blood increased by 2 to 4 times over those found in unsensitized mice of the same age. When TLA-sensitized and unsensitized mice were inoculated with Babesia, 4 of 10(40%) of the TLA-sensitized mice survived infection, while none of the unsensitized control mice live … More d longer than 14 days after infection. By contrast sensitization of nude mice with TLA has no effect on survival, and mice did not live more than 12 days. The number of thymic Thy-1, 2(+) cells decreased in TLA-sensitized and unsensitized BALA/c mice by almost 80% within 10 days after infection. During the same time, the numbers of B cells, T cells, and NK cells increased in the spleen, liver and peripheral blood of both sensitized and unsensitized mice. Especially notable were increases in the numbers of Lyt-2,2 cells in the spleen and blood and increases in numbers of NK cells in the spleen, liver and blood in both TLA-sensitized and unsensitized mice. When spleen cells from TLA-sensitized and unsensitized mice were cultured in the presence or absence of TLA for 6 days, assays for cytotoxicity using NK-insensitive P-815 target cells and NK-sensitive YAC-1 target cells demonstrated higher rates of cytotoxicity in cultures of TLA-sensitized spleen cells. The present results showed clearly that addition of TLA to cultures of TLA-sensitized spleen cells stimulates production of killer cells, such as lymphokine activated killer (LAK) cells and NK-like LAK and/or NK cells. Consequently, resistance of TLA sensitized mice to protozoan infection may be mediated not only by increases in the numbers of Thy-1, 2(+) and asialo GM1(+) cells, but also by quantitative and qualitative variations in these cell types. Less
据报道,刚地弓形虫可刺激对啮齿动物疟疾和疟疾的非特异性抵抗力。用弓形虫裂解物抗原(TLA)全身治疗的小鼠对伯氏疟原虫和罗达巴氏疟原虫感染具有高度抗性。TLA致敏后,间隔2周肌注TLA致敏的BALB/C小鼠外周血淋巴细胞中Sig(+)、Thy-1(+)、Lyt-2、2(+)、脾、肝和外周血中的去唾液酸GM 1(+)细胞比同龄未致敏小鼠增加2 ~ 4倍。当用巴氏杆菌接种致敏和未致敏的小鼠时,10只(40%)的致敏小鼠中有4只存活,而未致敏的对照小鼠无一存活 ...更多信息 d感染后14天以上。相比之下,用TLA致敏裸鼠对存活率没有影响,小鼠存活不超过12天。感染后10天内,致敏和未致敏的巴拉/c小鼠胸腺Thy-1,2(+)细胞数量减少近80%。同时,致敏和未致敏小鼠脾、肝和外周血中的B细胞、T细胞和NK细胞数量均增加。尤其值得注意的是,在TLA致敏和未致敏小鼠中,脾脏和血液中Lyt-2,2细胞的数量增加,以及脾脏、肝脏和血液中NK细胞的数量增加。当来自TLA致敏和未致敏小鼠的脾细胞在存在或不存在TLA的情况下培养6天时,使用NK不敏感的P-815靶细胞和NK敏感的YAC-1靶细胞的细胞毒性测定表明,在TLA致敏的脾细胞的培养物中,细胞毒性的比率更高。本研究结果清楚地表明,在TLA致敏的脾细胞培养物中加入TLA可刺激杀伤细胞的产生,如淋巴因子激活的杀伤(LAK)细胞和NK样LAK和/或NK细胞。因此,TLA致敏小鼠对原生动物感染的抗性可能不仅通过Thy-1,2(+)和去唾液酸GM 1(+)细胞数量的增加介导,而且通过这些细胞类型的定量和定性变化介导。少
项目成果
期刊论文数量(39)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Igarashi, I. et al.: "Changes of lymphocyte subpopulations and natural killer cells in mice sensitized with TLA before and after Babesia infection" Jpn. J. Vet. Sci.52. 969-977 (1990)
Igarashi, I. 等人:“在巴贝虫感染前后用 TLA 致敏的小鼠中淋巴细胞亚群和自然杀伤细胞的变化”Jpn。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
鈴木 直義: "実験動物のバベシア病(獣医住血微生物病)" 近代出版社、東京, (1988)
Naoyoshi Suzuki:“实验动物巴贝斯虫病(兽医血吸虫病)”Kindai Shuppansha,东京,(1988 年)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Miyahara,K; Igarashi,M.; Inomata; Sakurai,H.;Saito,A; Hirose,T.; Suzuki,N.: Zbl. Bakt. Hyg. Original A. 265. (1988)
宫原K;
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sakurai,H.et al.: "Long term in vitro suspension culture of <Toxoplasma>___ー and Hela Cells" Zbl.Bakt.Hyg.A.264. 468-477 (1987)
Sakurai, H. 等人:“<弓形虫>____ 和 Hela 细胞的长期体外悬浮培养”Zbl.Bakt.Hyg.A.264 (1987)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
鈴木直義ら: "マウス・マクロファ-ジの培養と細胞活性の測定" 家畜生化学研報. 23. 33-44 (1989)
Naoyoshi Suzuki 等人:“小鼠巨噬细胞的培养和细胞活性的测量”牲畜生物化学研究报告 23. 33-44 (1989)。
- DOI:
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- 影响因子:0
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SUZUKI Naoyoshi其他文献
SUZUKI Naoyoshi的其他文献
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{{ truncateString('SUZUKI Naoyoshi', 18)}}的其他基金
Developing a Learning Support System for Remote Body Movement Instruction : An Application in Physical Assessment Skill Training
开发远程身体运动指导学习支持系统:在体能评估技能训练中的应用
- 批准号:
20300270 - 财政年份:2008
- 资助金额:
$ 17.34万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on newly synthesized peptide as an immunoregulator against protozoan infection
新合成肽作为抗原虫感染免疫调节剂的研究
- 批准号:
06556053 - 财政年份:1994
- 资助金额:
$ 17.34万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular Immunological Studies on The Prevention and Diagnoses of Protozoan Infection
原虫感染预防和诊断的分子免疫学研究
- 批准号:
03556041 - 财政年份:1991
- 资助金额:
$ 17.34万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Studies on no-specifical immunoregulatory effects to the host by the addministration of newly activated-peptides derived from Toxoplasma lysate substance
弓形虫裂解物衍生的新激活肽对宿主的非特异性免疫调节作用的研究
- 批准号:
03404013 - 财政年份:1991
- 资助金额:
$ 17.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
Studies on the non-specific immunomodulator effects in animals by treatment of Toxoplasma lysate antigens
弓形虫裂解物抗原治疗动物非特异性免疫调节作用的研究
- 批准号:
59440017 - 财政年份:1984
- 资助金额:
$ 17.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)
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鱼类免疫增强剂快速评价方法的开发。
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08556032 - 财政年份:1996
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IL-12 AS AN IMMUNOPOTENTIATOR IN LEISHMANIASIS
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2071885 - 财政年份:1994
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6614725 - 财政年份:1994
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7581926 - 财政年份:1994
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IL-12 as an immunopotentiator in leishmaniasis
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