Studies on Macrobial Metabolites Effective to Eucaryotic Cells

YAMAZAKI从Emericella navahoensis和Talaromyces lutens的代谢产物中分离出单胺氧化酶氧化酶抑制剂EA-1、TA-1、TL-1和去甲异喹啉酸，并阐明了它们的特异活性。OMURA从放线菌的代谢产物中寻找血小板聚集抑制剂，并在几种醌类抗生素如Medermycin和Nanaomycin中发现了这种活性。还鉴定了一个新的等四环素体OM-4842。SUZUKI测定了N-乙酰孢菌素的总结构和刺孢霉素的部分结构。MIKAMI合成了抗肿瘤抗生素沙夫霉素的衍生物，并对其构效关系进行了研究。YAMAMOTO从栗生大茎点菌（Macrophoma castaneicola）中分离得到单孢菌（monorden），从Glomellera cingulate中分离得到酪醇（tyrosol），从灰葡萄孢（Botrytis cinerea）中分离得到降葡萄糖醛（norbotryal）等。中村确定了抗真菌抗生素新enactins的结构。对他们的活动进行了评价。山口通过使用白色念珠菌的野生型和突变株的筛选系统寻找抗真菌抗生素，并分离出化合物。NATORI研究拟茎点霉属的代谢产物，并分离出多种细胞松弛素类化合物。SATO发现己烯雌酚（DES）对肌小管组装有影响。研究了己烯雌酚及其有关化合物的作用。NOZOE对担子菌的代谢产物进行了研究，分离出了多种抗生素。FUJITA研究了含有-氨基-异丁酸作为其组分的肽，并确定了它们的结构。OKUDA和IWASAKI分离了几种抗有丝分裂化合物，并研究了它们的作用方式。还建立了一个利用梨孢菌进行筛选的系统。

YAMAZAKI isolated monoamine oxidase oxidase inhibitors, EA-1, TA-1, TL-1 and norsolorinic acid, as the metaboltes Emericella navahoensis and Talaromyces lutens, and their specific sctivity was clarified. OMURA searched for pletelet aggregation inhibitors form the metaboltes of Actinomycetes, and found such activity in several quinone antibiotics such as medermycin and nanaomycin. A new isotetracenoid, OM-4842, was slso identified. SUZUKI determined the total structure of N-acetylsporaviridin and the partial atructure of aculeximycin. MIKAMI prepared a vriety of derivatives of saframycin, an antitumor antibiotic, and structure-activity relationship was studied. YAMAMOTO searched for anti-L5178Y cell compounds, and isolated monorden from Macrophoma castaneicola, tyrosol from Glomellera cingulate and norbotryal etc from Botrytis cinerea. NAKAMURA determined the structures of neo-enactins, antifungal antibiotics. Their activities were evaluated. YAMAGUCHI searched for antifungal antibiotics by a screening system using a wild type and a mutant strains of Candida albicans and isolated a compound. NATORI studied on the metabolites of Phomopsis sp., and isolated a varietyof cytochalasan compounds. SATO found that diethylstibestrol (DES) effects on myclotubule assembly. The action of DES and related compounds were studied. NOZOE studied on the metabolitesd of Basidiomycete, and isolated several antibiotics. FUJITA studied on the peptidic containing -amino-isobutylic acid as their components, and their structures were established. OKUDA and IWASAKI isolated several anti-mitotic compounds and their mode of action was studied. A screening system using Pyricularia oryzae was also deviced.