Specific metabolisms of Bombyx mori and its application for production

The silkworm, Bombyx mori是良好的domesticated insect which silk protein as its bioproduct. To develop thesilk protein和其他生物的productivitythe specific metabolisms of this insect as well as in insecta have been investigated in thisresearch. The results obtained are as follows：1. A silkworm peptide hormone, diapause hormone规则cyclic GMP metabolism at the different way from the mammalian peptide hormone. Calmodulinsilkworms is specified in its deficiency of trimethyl-lysine. 2. Protease and its inhibitors arepresent in silkworm hemolymph and their dynamic changes according to development and with theinjection of microorganisms led us to conclude that this system is functionable in histolysisprotection against pathogens. 3. The temperature-dependent shift in carbohydrate metabolism wasclarified in silkworm diapause eggs. Acclimation at 5c induced NAD-sorbitol dehydrogenase but noinduction took place at 0 C condition in both diapause and non-diapause eggs. 4. in addition to theseveral ecdysteroids, a specific sterol, bombycosterol， was标识silkworm ovaries，suggesting the occurrence of specific steroid metabolism. 5. extra-cellular glycolysis in cellular glycolysisspermatophore is shown to contribute to supply metabolic energy for sperm maturation or motionArginine也可以用来作为能量来源，用于这个用途，其中Arginine是provided from proteinthrough a specific protease action。

The silkworm, Bombyx mori, is the well domesticated insect which provides silk protein as its bioproduct. To develop the productivity of silk protein and other bioresources, the specific metabolisms of this insect as well as in insecta have been investigated in this research. The results obtained are as follows: 1. A silkworm peptide hormone, diapause hormone, regulates cyclic GMP metabolism at the different way from the mammalian peptide hormone. Calmodulin of silkworms is specified in its deficiency of trimethyl-lysine. 2. Protease and its inhibitors are present in silkworm hemolymph and their dynamic changes according to development and with the injection of microorganisms led us to conclude that this system is functionable in histolysis and protection against pathogens. 3. The temperature-dependent shift in carbohydrate metabolism was clarified in silkworm diapause eggs. Acclimation at 5゜C induced NAD-sorbitol dehydrogenase but no induction took place at 0゜C condition in both diapause and non-diapause eggs. 4. In addition to the several ecdysteroids, a specific sterol, bombycosterol, was identified in silkworm ovaries, suggesting the occurrence of specific steroid metabolism. 5. The extra-cellular glycolysis in spermatophore is shown to contribute to supply metabolic energy for sperm maturation or motion. Arginine is also used as energy source for this purpose in which arginine is provided from protein through a specific protease action.