Biotechnological studies on functional development of the silkworm, Bombyx mori.

家蚕功能发育的生物技术研究。

• 批准号: 02304020
• 负责人: YAMASHITA Okitsugu
• 金额: $ 8.77万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02304020/
• 关键词: larval serum protein; 30k protein; storage protein l; protease inhibitor; aldolase; sorbitol dehydrogenase; initiatolin; prothoracicotropic hormone; プロテアーゼインヒヒター; 家蚕; 幼虫特異タンパク質; 貯蔵タンパク質; プロテア-ゼインヒビタ; ソルビト-ルデヒドロゲナ-ゼ; イニンアトリン; アルドラ-ゼ; 卵特異タンパ

In this project research, 7 proteins which have unique function in silkworms have been selected for the comprehensive elucidation of their primary structure, gene organization and regulation of the gene expression. The Bombyx mori larval serum protein was found to be a new protein which characterizes the larval life. A pupal serum protein, 30k protein, was shown to be biosynthesized by expression of the gene under the influence of the 5^1promoter sequence. The expression of storage protein 1 gene was stimulated by a nuclear proteins of larval fat body, although the biochemical properties of the proteins remain unknown. Protease inhibitors isolated from silkworm hemolymph were demonstrate to be produced by the regulation of insect hormones which stimulate expression of the protease inhibitor gene. There was a rapid change in isozyme pattern of aldolases during embryogenesis of silkworms. Sorbitol dehydrogenese, which was a rate-limiting enzyme for sorbitol degradation at diapause termination, was produced by the transcription of the gene. Initiatolin, a protease responsible for sperm activation was identifies in spermatophore of silkworms and characterized biochemically. A neuropeptide hormone, prothoracicotropic hormone, was mapped to 2.7 of the 22nd linkage group of silkworms, and diapause hormone was to the 11th linkage.

本课题选择了7种在家蚕中具有独特功能的蛋白质，对其一级结构、基因组织和基因表达调控进行了全面的研究。家蚕幼虫血清蛋白是一种新的反映家蚕幼虫生活特征的蛋白质。在5^1启动子序列的影响下，蛹血清蛋白30 k蛋白通过基因的表达而被生物合成。贮藏蛋白1基因的表达受幼虫脂肪体核蛋白的刺激，但该蛋白的生化性质尚不清楚。从家蚕血淋巴中分离的蛋白酶抑制剂被证明是通过刺激蛋白酶抑制剂基因表达的昆虫激素的调节而产生的。在家蚕胚胎发育过程中，醛缩酶同工酶谱发生了迅速的变化。山梨醇脱氢酶是滞育终止时山梨醇降解的限速酶，由该基因转录产生。在家蚕精荚中发现了一种与精子活化有关的蛋白酶-起始素。家蚕第22连锁群的第2.7连锁群上有一种神经肽激素--促前胸腺激素，第11连锁群上有滞育激素。

项目成果

Kameoka,K.et al.: "A quantitative analysis of radiation-induced chromosome aberrarions with a fluorescent digital image microscope." J.Rad.Res.33. 71-79 (1992)

Kameoka,K.et al.：“使用荧光数字图像显微镜对辐射引起的染色体畸变进行定量分析。”

Kajiura,Z.and Yamashita,O: "Induction of metamorphosis of the methoprene-induced dauer larvae of the silkworm, Bombyx mori, by ecdysteroids." Comp. Biochem. Physiol. 101A. 227-280 (1992)

Kajiura,Z. 和 Yamashita,O：“通过蜕皮类固醇诱导家蚕 (Bombyx mori) 的甲虫酯诱导的 dauer 幼虫的变态。”

Okazaki,K.,Tsuchida,K.,Maekawa,H.,Ishikawa,H.,and Fujiwara,H: "Identification of a pentanucleotide telomeric sequence, (TTAGG)n, in the silkworm, Bombyx mori and other insects." J.Molec.Cell.Biol.

Okazaki,K.、Tsuchida,K.、Maekawa,H.、Ishikawa,H. 和 Fujiwara,H：“鉴定蚕、家蚕和其他昆虫中的五核苷酸端粒序列 (TTAGG)n。”

Niimi,T.and Yaginuma,T: "Biosynthesis of NAD-sorbitol dehydrogenese is induced by acclimation at 5ﾟC in diapause eggs of the silkworm, Bombyx mori." Comp.Biochem.Physiol. 102B. 169-173 (1992)

Niimi, T. 和 Yaginuma, T：“NAD-山梨糖醇脱氢酶的生物合成是通过家蚕滞育卵在 5℃ 下的驯化诱导的。Comp.Biochem.Physiol 169-173 (1992)。

Choe,H.et al.: "Processing of structural polypeptides of infectious flacherie virus of the silkworm,Bombyx mori:VP1 and VP4 are derived from VPO." J.Invert.Pathol.60. 113-116 (1992)

Choe,H.et al.：“家蚕传染性 Flacherie 病毒结构多肽的加工，Bombyx mori：VP1 和 VP4 源自 VPO。”