The allosteric effect in hemoglobin.

1)本文研究了脱氧血红蛋白分子的氧亲和力被异常血红蛋白和化学修饰血红蛋白改变后，其各部分构象的变化。结果发现，血红素周围的构象变化和珠蛋白部分的构象变化并没有发生一致的方式，相反的两个状态变构模型的预期。2)发现四种金属原卟啉可模拟脱氧血红素。其中Ni-原卟啉IX的研究最为广泛。从Ni-Fe混合血红蛋白的氧平衡性质判断。Ni-原卟啉IX不仅在未修饰的血红蛋白中而且在修饰的血红蛋白中表现出类似于“冷冻”脱氧血红素的行为。3)通过使用Ni-原卟啉制备的血红蛋白氧合的中间状态的模型显示构象状态，这不能在两状态变构模型的框架中被容纳。4)用铁系过渡金属离子取代铁，研究了铁的电子态与球蛋白分子的相互作用。具有金属原卟啉的血红蛋白的构象状态根据元素在周期表中的位置而改变。对结果没有给出完整的解释。5)获得的数据表明11相互作用的重要性。6)89组氨酸显示出贡献约25%的碱性玻尔效应。7)通过对E.杆菌8)建立了一套紫外激光激发的共振拉曼光谱测量装置。9)通过构象能量函数的计算机计算，分析了肌红蛋白的动态三维结构。

1) The conformational changes of various parts ot a deoxyhemoglobin molecule were investigated when its oxygen affinity was altered by use of abnormal hemoglobins and chemically modified ones. It was found that the conformational changes around heme and those of the globin moiety did not take place in concerted manner on the contrary to the expectation of the two state allosteric model. 2) Four metalloprotoporphyrins were found to medel the deoxyheme. Among them Ni-protoporphyrinIX has been studied most extensively. Judging from the oxygen equilibrium properties of Ni-Fe hybrid hemoglobins. Ni-protoporphyrinIX behaves just like "frozen" deoxyheme not only in unmodified hemoglobins but also in modified ones. 3) The model for the intermediate state of the oxygenation of hemoglobin prepared by use of Ni-protoporphyrin showed a conformational state, which could not be accomodated in the framework of the two state allosteric model. 4) The interaction between the electronic state of iron and the globin moiety was investigated by substituteing iron serries transition metal ions for iron. The conformational state of the hemoglobin having the metalloprotoporphyrin changed according to the position of the element in the periodical table. No complete explanation of the result has been given. 5) Data were obtained to suggest the importance of the 1 1 interaction. 6) 89 Histidine was shown to contribute about 25% of the alkaline Bohr effect. 7) It became possible to prepare a hemoglobin having any primary structure by gene manipulation of E. coli. 8) An equipment to measure a resonance Raman spectra excited by ultraviolet laser beams was constructed. 9) The dynamic three dimentional structure of myoglobin was analyed by the computer calculation of the conformational energy function.