SP4 - Cross talk between environmental conditions, oaktree performance, and the microbiomes of their soil, endo- and phyllosphere

SP4 - 环境条件、橡树性能及其土壤、内叶圈和叶际微生物组之间的交互作用

• 批准号: 520751609
• 负责人: Professorin Dr. Anke Becker
• 金额: --
• 依托单位: Zentrum für Synthetische Mikrobiologie (SYNMIKRO)
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/520751609?language=en
• 关键词: SP4; Cross; talk; between; environmental

The plant microbiome, i.e. the community of associated microorganisms, plays a crucial role for mutual acclimation to environmental changes and the subsequent evolutionary adaptation (A&A). Beneficial microbes can enhance plant tolerance traits against stress, which reinforces shoot and root protection by modifying phytohormonal levels, antioxidant and enzyme production, osmotic adjustment or biofilm formation to increase water and nutrient uptake, gas exchange and water use efficiency. Since these findings were mainly retrieved from studies on crops or herbaceous plants, it remains little investigated how trees and their microbiomes react to environmental changes, including more frequent and intensified droughts and pathogenic pressures. Hence, SP4 aims to investigate, how oak-associated microorganisms respond to various environmental conditions and stresses. Considering the endogenous rhythmic growth and the modularity of the oak, SP4 intends to determine an oak core microbiome and characterize key stone taxa, which enhance resilience against the applied stressors. To do so, SP4 bridges correlative links revealed from field analyses (SP4a), and experiments with individual bacterial strains and synthetic communities deciphering causal mechanisms (SP4b). Thereby, SP4a uses molecular sequencing tools to monitor bacteria and fungi associated with above- and belowground compartments of DF159 saplings in Ecotron and Tree Canopy Exposition experiments, and along the PhytOakmeter field monitoring sites. SP4b performs microcosm experiments under containment and controlled conditions, which allow experimental testing of hypotheses on microbial effects on trees’ A&A. By integrating complementary data, and using the synergic effort within the research unit, SP4 plans to add to the general knowledge on tree microbiomes, and specifically in understanding beneficial microbial impacts across Phytoakmeter saplings along environmental variation.

植物微生物组，即相关微生物群落，在相互适应环境变化和随后的进化适应（A&A）中起着至关重要的作用。有益微生物可以通过调节植物激素水平、抗氧化剂和酶的产生、渗透调节或生物膜的形成来增加水分和养分的吸收、气体交换和水分利用效率，从而增强植物对逆境的耐受特性，加强对茎和根的保护。由于这些发现主要来自于对作物或草本植物的研究，因此对树木及其微生物组如何对环境变化（包括更频繁和加剧的干旱和致病压力）作出反应的研究仍然很少。因此，SP4旨在研究橡树相关微生物如何应对各种环境条件和胁迫。考虑到栎树内源生长的节律性和模块化，SP4计划确定栎树核心微生物群，并对关键的石质分类群进行表征，以增强对外界胁迫的抵御能力。为了做到这一点，SP4连接了从现场分析（SP4a）和单个菌株和合成群落的实验中揭示的相关联系，以破译因果机制（SP4b）。因此，SP4a利用分子测序工具监测与Ecotron和Tree Canopy Exposition实验中DF159树苗的地上和地下隔间相关的细菌和真菌，以及PhytOakmeter现场监测点。SP4b在封闭和受控条件下进行微观实验，允许对微生物对树木A&A影响的假设进行实验测试。通过整合互补数据，并利用研究单位内部的协同努力，SP4计划增加对树木微生物组的一般知识，特别是了解沿环境变化的Phytoakmeter树苗的有益微生物影响。