Membrane structure of Golgi apparatus, chloroplasts and mitochondria observed by freeze-substitution

冷冻置换观察高尔基体、叶绿体和线粒体的膜结构

• 批准号: 60540447
• 负责人: UEDA Katsumi.
• 金额: $ 1.15万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60540447/
• 关键词: Chloroplast membrane; Golgi membrane; Freeze-substitution; mitochondrial membrane; Ultrastructure; 微細構造

The membrane structure of Golgi apparatus, chloroplasts and mitochondria in Scenedesmus acuminatus was studied by the freeze-substitution method. Most of these membranes clearly exhibited three-layered structures. It was interpreted that the three layers were visualized by resolving the lipid component from membranes remaining electron dense membrane proteins. The upper and lower membranes of the distal Golgi cisternae were in close contact with each other at the cisternal centre. Similar contact of two membranes was also observed in thylakoids. The electron density of transfer vesicles, secretory vesicles, and cisternal margins was very high. These findings differ considerably from those obtained by conventional chemical fixation, and suggest that freeze-substitution is superior to chemical fixation for preserving structures without distortion and preventing dissolution of constituent substances.

采用冷冻置换法对尖生栅藻高尔基体、叶绿体和线粒体的膜结构进行了研究。这些膜中的大多数明显表现出三层结构。据解释，这三个层是通过从膜上分离出脂质组分而显现出来的，剩下的是电子致密膜蛋白。高尔基池远端的上膜和下膜在池中心相互紧密接触。在类囊体中也观察到类似的两层膜接触。转运囊泡、分泌囊泡和脑池边缘的电子密度很高。这些研究结果与通过常规化学固定获得的结果有很大不同，并且表明冷冻置换上级化学固定，以保持结构不变形并防止组成物质溶解。

项目成果

Ueda, Katsumi, and Noguchi, Tetsuko: "Structure of dictyosomes in Scenedesmus acuminatus observed by the freeze-substitution method" Journal of Cell Science. 82. 217-222 (1986)

Ueda, Katsumi 和 Noguchi, Tetsuko：“通过冷冻替代法观察到的尖栅藻中网状体的结构”《细胞科学杂志》。

Ueda,K.;Noguchi,T.: Journal of Cell Science. 82. 217-222 (1986)

上田，K.；野口，T.：细胞科学杂志。