权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Centriolar Behavior and Flagellum Formation in Cell Culture System of Testicular Cells of the Sea Urchin

海胆睾丸细胞培养体系中的中心粒行为和鞭毛形成

基本信息

批准号：
60540465
负责人：
ISHIKAWA Masaru
金额：
$ 1.22万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60540465/
关键词：
Sea Urchin Spermatogenesis Cell Culture Centriole 鞭毛形成

项目摘要

Centriolar behavior and flagellum formation during maturation division of male germ cells were studied by using of cultured testicular cells of the sea urchin, Hemicentrotus pulcherrimus.1. Isolated germ cells from the testis were divided by Ficoll density gradient centrifugation into the following 3 cell type fractions : the primary spermatocytes together with spermatogonia, secondary spermatocytes and spermatids. Each cell fraction was then cultured in a culture medium.2. A flagellum was detected in the spermatogonia, the primary and secondary spermatocytes at eary prophase. In this stage, 2 centrioles including a basal body duplicate to form 2 paires of centrioles at the base of the flagellum. At the time of prometaphase, the flagellum was absorbed into the cytoplasm and a pair of centrioles was located at each pole of the first and second division. After the second division, 4 spermatids with a flagellum were formed and developed to spermatozoa.3. When nuclear division was reversibly suppressed by cycloheximide, each of the primary and secondary spermatocytes sprouted frequently 2-4 flagella.4. From above-mentioned results, it was concluded that in spermatogonia and spermatocytes of the sea urchin, duplication of the centriole takes place in association with flagellum growth, and then each paired centriole changes its function to form the cell division centers.

以培养的马粪海胆（Hemicentrotus pulcherrimus）睾丸细胞为材料，研究了雄性生殖细胞成熟分裂过程中的着丝粒行为和鞭毛形成.用Ficoll密度梯度离心法将睾丸生殖细胞分为初级精母细胞、精原细胞、次级精母细胞和精子细胞3种类型。然后将每个细胞部分在培养基中培养。在早期的精原细胞、初级精母细胞和次级精母细胞中发现鞭毛。在此阶段，2个中心粒包括一个基体在鞭毛基部复制形成2对中心粒。在前中期，鞭毛被吸收到细胞质中，第一次分裂和第二次分裂的两极各有一对中心粒。第二次分裂后，形成4个带鞭毛的精子细胞，并发育为精子.当放线菌酮可逆性抑制核分裂时，初级和次级精母细胞各萌发2-4个鞭毛.上述结果表明，在海胆精原细胞和精母细胞中，中心粒的复制与鞭毛的生长有关，然后每对中心粒改变其功能，形成细胞分裂中心。