The studies on the host specificity of leaf beetles

以水杨科植物为食的叶甲虫（lochmaae capreae cribrata）不能以一些水杨科植物为食，如：全柳（Salix integra）、S. chaenomeloides和S. koriyanagi。绿原酸是一种对这种昆虫具有摄食威慑作用的物质，它是从整片菊叶的水渗出物中分离出来的。绿原酸含量较高的是整条、卷叶参和红柳。该化学物质对被这些植物所接受的异色弹虱没有起到摄食威慑作用。这些结果表明，水杨树对这三种水杨科植物的排斥作用取决于高水平的绿原酸。在比较各种叶甲虫对绿原酸的摄食行为时，似乎许多物种对这种化学物质的摄食威慑有反应。这些事实表明，广泛分布于植物界的绿原酸在叶甲虫寄主特异性中起着化学线索的作用。研究了7种叶甲虫对寄主植物叶片表面常见蜡质成分、正构烷烃、正醇和正酸的取食反应。在不同寄主范围的7个物种中，有5个被这些蜡成分刺激进食。<C_(21)> - <C_(25)>和<C_(30)> - <C_(33)>的正构烷烃刺激了白桦；<C_(22)> - <C_(24)>正醇；<C_(31)> - <C_(35)>正构烷烃和<C_(24)> - <C_(26)>正构酸；<C_(27)> - <C_(30)>正构烷烃；葎草由<C_(27)> - <C_(31)>正构烷烃组成。气相色谱分析表明，具有取食刺激活性的蜡质成分在各寄主植物叶片中均占优势。这些结果表明，植物叶片表面的蜡质成分在叶甲虫的寄主特异性中起着化学线索的作用。

Salicaceae-feeding leaf beetle, Lochmaeae capreae cribrata is prevented from feeding on a few salicaceous plants such as Salix integra, S. chaenomeloides and S. koriyanagi. Chlorogenic acid was isolated as a feeding deterrent to this insect from the water exudates of S. integra leaves. Chlorogenic acid was contained at high levels in S. integra, S. chaenomeloides and S. koriyanagi. This chemical did not acted as a feeding deterrent to Plagiodera versicolora distincta being accepted these plants. These results suggest that the reject of these three salicaceous plants by L. c. cribrata depends on the high levels of chlorogenic acid. In comparing the feeding behavior of various leaf beetles to chlorogenic acid, it appeared that many species responded to the chemical as a feeding deterrent. These facts suggest that chlorogenic acid widely distributed in the plant kingdom acts as a chmical cue in the host specificity of leaf beetles.Feeding responses of seven leaf beetle species to common wax components of host plant leaf surface, n-alkanes, n-alcohols and n-acids were investigated. Five out of seven species which have different host ranges were stimulated to feed by these wax components. Atrachya menetriesi was stimulated by <C_(21)> - <C_(25)> and <C_(30)> - <C_(33)> n-alkanes; Chrysolina aurichalcea by <C_(22)> - <C_(24)> n-alcohols; Galerucella vittaticollis by <C_(31)> - <C_(35)> n-alkanes and <C_(24)> - <C_(26)> n-acids; Plagiodera versicolora distincta by <C_(27)> - <C_(30)> n-alkanes; Pyrrhalta humeralis by <C_(27)> - <C_(31)> n-alkanes. Gaschromatographic analysis indicated that the wax components showing feeding stimulative activities were tend to dominant in each host plant leaves of these insects. These results suggest that the wax components present on plant leaf surface act as chemical cues in the host specificity of leaf beetles.