The studies on the host specificity of leaf beetles

柳科食叶甲Lochmaeaecapalicribrata不能在一些柳科植物上取食，如全缘柳chaenomeloides和S.郡柳。绿原酸作为一种拒食物质，是从该虫的分泌物中分离得到的。integra叶。绿原酸在S. integra、S. chaenomeloides和S.郡柳。这种化学品并没有作为一个喂养威慑Plagiodera versicolora distincta被接受这些植物。这些结果表明，柳珊瑚对这三种柳科植物的排斥是由其自身的生理特性引起的。C. cribrata依赖于高水平的绿原酸。在比较各种叶甲的取食行为，绿原酸，似乎许多物种响应化学品作为一种取食威慑。研究了7种叶甲对寄主植物叶面常见蜡质成分、正构烷烃、正构醇和正构酸的取食反应。其中五个具有不同的寄主范围的物种，刺激这些蜡成分的饲料。C_（21）> -C_（25）>和C_（30）> -C_（33）>正构烷烃对米氏圆盖藻有刺激作用，C_（22）> -C_（24）>正构醇对金囊藻有刺激作用，C_（31）> -C_（35）>正构烷烃和C_（24）> -C_（26）>正构酸对条颈小盖藻有刺激作用;异色斜甲（Plagiodera versicolora distincta）为<C_（27）> - <C_（30）>正构烷烃，肱骨毛蚜（Pyrrhalta humeralis）为<C_（27）> - <C_（31）>正构烷烃。气相色谱分析表明，各寄主植物叶片中具有取食刺激活性的蜡质成分均占优势。这些结果表明，植物叶片表面的蜡质成分作为化学线索在叶甲的寄主特异性。

Salicaceae-feeding leaf beetle, Lochmaeae capreae cribrata is prevented from feeding on a few salicaceous plants such as Salix integra, S. chaenomeloides and S. koriyanagi. Chlorogenic acid was isolated as a feeding deterrent to this insect from the water exudates of S. integra leaves. Chlorogenic acid was contained at high levels in S. integra, S. chaenomeloides and S. koriyanagi. This chemical did not acted as a feeding deterrent to Plagiodera versicolora distincta being accepted these plants. These results suggest that the reject of these three salicaceous plants by L. c. cribrata depends on the high levels of chlorogenic acid. In comparing the feeding behavior of various leaf beetles to chlorogenic acid, it appeared that many species responded to the chemical as a feeding deterrent. These facts suggest that chlorogenic acid widely distributed in the plant kingdom acts as a chmical cue in the host specificity of leaf beetles.Feeding responses of seven leaf beetle species to common wax components of host plant leaf surface, n-alkanes, n-alcohols and n-acids were investigated. Five out of seven species which have different host ranges were stimulated to feed by these wax components. Atrachya menetriesi was stimulated by <C_(21)> - <C_(25)> and <C_(30)> - <C_(33)> n-alkanes; Chrysolina aurichalcea by <C_(22)> - <C_(24)> n-alcohols; Galerucella vittaticollis by <C_(31)> - <C_(35)> n-alkanes and <C_(24)> - <C_(26)> n-acids; Plagiodera versicolora distincta by <C_(27)> - <C_(30)> n-alkanes; Pyrrhalta humeralis by <C_(27)> - <C_(31)> n-alkanes. Gaschromatographic analysis indicated that the wax components showing feeding stimulative activities were tend to dominant in each host plant leaves of these insects. These results suggest that the wax components present on plant leaf surface act as chemical cues in the host specificity of leaf beetles.