Search for Cytotoxic Substances from Marine Sponges

从海洋海绵中寻找细胞毒性物质

• 批准号: 60560212
• 负责人: FUSETANI Nobuhiro
• 金额: $ 1.09万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60560212/
• 关键词: Marine sponges; Cytotoxic; Starfish egg assay; Tumor cells; Actin polymerization; Spongionella; Fasciospongia; Fasclospongia

Marine sponges (100 specimens) collected from various places along the Pacific coasts of Japan were tested for inhibition in the starfish egg assay. A total of 31 specimens, including 20 fat soluble and 23 water soluble fractions, were active, among which 6 sponges were found to be promising in P388 or L1210 leukemia test.From two sponges which were active in the starfish egg assay, four active compounds have been isolated and their structures elucidated. Okinonellins A (1) and B (2) were isolated from a sponge of the genus Fasciospongia which was collected in Uwa Sea. Both compounds inhibited cell division of fertilized starfish eggs at 5 g/ml by affecting actin polymerization. Two sesterterpenes named spongionellin (3) and dehydrospongionellin (4) which inhibited development of starfish embryos at 2 g/ml have been isolated from Spongionella sp. collected in the Izu Peninsula.

从日本太平洋沿岸沿着不同地方采集的海绵（100个样本）在海星卵试验中进行了抑制试验。结果表明，共分离得到31个活性组分，其中脂溶性组分20个，水溶性组分23个，其中6个组分对P388或L1210白血病细胞有较好的活性，另外2个组分对海星卵有较好的活性，从中分离得到4个化合物，并鉴定了它们的结构。Okinonellins A（1）和B（2）是从日本宇和海采集的片海绵属海绵中分离得到的。这两种化合物在5 μ g/ml时通过影响肌动蛋白聚合来抑制受精海星卵的细胞分裂。从采集于伊豆半岛的海绵藻中分离出两种二萜类化合物，分别命名为海绵藻素（spongionellin，3）和脱氢海绵藻素（dehydrospongionellin，4），其浓度为2 μ g/ml时对海星胚胎发育有抑制作用。

项目成果

Y. Kato: "Okinonellins A and B, two novel furanosesterterpenes, which inhibit cell division of fertilized starfish eggs, from the marine sponge Spongionella sp." Experientia. 42. 1299-1300 (1986)

Y. Kato：“Okinonellins A 和 B 是两种新型呋喃糖酯萜烯，可抑制来自海洋海绵 Spongionella sp. 的受精海星卵的细胞分裂。”

Y.Kato: Chemistry Letters. 1521-1524 (1985)

Y.Kato：化学快报。

Y.Kato: Experientia. 42. 1299-1300 (1986)

Y.Kato：体验。

Y. Kato: "Spongionellin and dehydrospongionellin, new furanosesterterpenes which inhibit cell division of fertilized starfish eggs, from the marine sponge Spongionella sp." Chemistry Letters. 1521-1524 (1985)

Y. Kato：“海绵内酯和脱氢海绵内酯是新的呋喃糖酯萜，可抑制受精海星卵的细胞分裂，来自海洋海绵 Spongionella sp。”