Estrogen and progesterone receptors in canine mammary tumors.

犬乳腺肿瘤中的雌激素和孕激素受体。

• 批准号: 60560330
• 负责人: SASAKI Nobuo
• 金额: $ 1.28万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60560330/
• 关键词: mammary tumor; mammary tissue; canine; estrogen receptor; progesterone receptor; cytosol; プロジェステロンレセプター

Thirty-seven canine mammary tumors with various histologic types were determined their cytosol estrogen and progesterone receptors(ER, PR) by the dextran-coated charcoal method. Out of the tumors, 64.7% showed ER positive(binding sites were more than 2 fmol/mg protein). The mean binding sites and dissociation constant(Kd) were 8.8 fmol/mg protein and 1.17 x <10^(-9)> M respectively. PR was detected in 71.0% of tumors examined(more than 5 fmol/ mg protein). The mean binding sites and Kd were 24.3 fmol/mg protein and 2.15 x <10^(-9)> M. And 90% of ER positive specimens were PR positive. Histopathologically, 35.5% of tumors were benign and the rest were malignant. Also 75.7% were epithelial origin. In benign tumors, 76.9% were ER positive and 90.9% were PR positive. But in malignant tumors, only 58.3% and 60.9% showed ER and PR positive, respectively. These results were quite similar to the receptor data reported in human and dogs. This suggests that the endocrine therapy in human may als … More o be effective in canine mammary tumors.In the next experiment, the correlation of receptors between neoplastic and non-neoplastic mammary tissues was investigated. Furthermore, 14 normal mammary tissues from healthy female dogs with normal estrous cycles were examined for the presence of the receptors as a control. Histopathologic examination revealed that 4 of 15 tumor bearing mammary tissues examined were hyperplastic and that the rest were normal. There were no significant differences in ER contents between the tumor tissues and their surrounding tissues. On the contrary, PR contents in surrounding tissues were considerably lower than those in mammary tumor tissues. But the levels of ER and PR contents in the surrounding tissues were similar to those of normal mammary tissues from normal female dogs.From these results, the most significant difference in ER and PR between neoplastic and non-neoplastic mammary tissues was the content of PR. A prolonged administration of progesterone was reported to induce mammary tumors. Thus, it is suggested that progesterone as well as estrogen may play an important role in the etiology of neoplastic change in canine mammary tumors. Less

用葡聚糖-活性炭法测定了37例不同组织学类型的犬乳腺肿瘤细胞浆雌激素受体（ER）和孕激素受体（PR）。在肿瘤中，64.7%显示ER阳性（结合位点大于2fmol/mg蛋白）。平均结合位点和解离常数（Kd）分别为8.8 fmol/mg蛋白和1.17 x <10^（-9）> M。在71.0%的肿瘤中检测到PR（超过5 fmol/ mg蛋白质）。平均结合位点和Kd分别为24.3 fmol/mg蛋白和2.15 x <10^（-9）> M。90%的ER阳性标本PR阳性。组织学上，良性肿瘤占35.5%，其余为恶性。75.7%为上皮来源。良性肿瘤中ER阳性率为76.9%，PR阳性率为90.9%。而在恶性肿瘤中，ER和PR的阳性率分别为58.3%和60.9%。这些结果与人和犬中报告的受体数据非常相似。这表明，人类的内分泌治疗可能 ...更多信息 在接下来的实验中，研究了肿瘤性和非肿瘤性乳腺组织之间受体的相关性。此外，还检测了14只发情周期正常的健康雌性犬的正常乳腺组织中受体的存在作为对照。组织学检查发现15例乳腺癌组织中4例增生，其余均正常。癌组织与癌旁组织ER含量无显著性差异。癌旁组织中PR含量明显低于乳腺癌组织。而周围组织中ER、PR含量与正常雌犬乳腺组织中ER、PR含量相近，由此可见，乳腺癌组织与非癌组织中ER、PR含量的差异最大的是PR含量。因此，它是建议，孕激素以及雌激素可能发挥重要作用的病因学肿瘤的变化，在犬乳腺肿瘤。少

项目成果

Nobuo SASAKI,et al: Jpn.J.Vet.Sci.

Nobuo SASAKI 等人：Jpn.J.Vet.Sci。

Nobuo SASAKI, et al: "Estrogen and progesterone receptors in mammary tumors and their surrounding tissues in dogs." Jpn. J. Vet. Sci.

Nobuo SASAKI 等人：“狗乳腺肿瘤及其周围组织中的雌激素和孕激素受体。”

Nobuo SASAKI,et al: Jpn.J.Vet.Sci.,.

Nobuo SASAKI 等人：Jpn.J.Vet.Sci.,。

Nobuo SASAKI, et al: "Estrogen and progesterone receptors in canine mammary tumors." Jpn. J. Vet. Sci.

Nobuo SASAKI 等人：“犬乳腺肿瘤中的雌激素和孕激素受体。”