Relationship between metastasis of canine malignant melanoma and membrane glycoprotein AD-1 (CD 63)

犬恶性黑色素瘤转移与膜糖蛋白AD-1（CD 63）的关系

• 批准号: 10839004
• 负责人: SASAKI Nobuo
• 金额: $ 2.5万
• 依托单位: THE UNIVERSITY OF TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10839004/
• 关键词: dog; malignant melanoma; cell line; AD-1; monoclonal antibody; metastasis

Canine malignant melanoma has been known to be quite malignant with rapid growth, wide local invasion, early metastasis, resistant to chemotherapy and radiotherapy and quite early metastasis. In mice, membrane glycoprotein, AD-1 or CD 63, has been known that it appears in the primary lesion but disappears in the metastatic lesion, therefore may play a role in the mechanism of the tumor metastasis. The purpose of this study is to clarify the role of AD-1 in the metastasis of canine malignant melanoma.In the first experiment, establishment of melanoma cell lines from the canine patients were conducted. Four cell lines originated from the oral membrane and skin with different proliferation manner and transplantability to nude mice were established.Using one of these cells, production of the monoclonal antibody to AD-1 was conducted. As a result, two antibodies were obrtained. One reacted only to melanoma cells and the other reacted to both melanoma and mastocytoma cells, which are known to have AD-1. Unfortunately it is still not demonstrated whether these antibodies are specific to AD-1.These two antibodies were used for the histochemistry of spontaneous canine melanoma tissues obtained at surgery. As a result, the former antibody reacted to either primary or metastatic lesions ; in some specimens, positive in the primary tissues and negative in the metastatic tissues, whereas in other tissues, positive in the metastatic tissues and negative in the primary tissues. This may suggest this antigody recognizes some of the substance which may relate to metastasis, but perhaps not to AD-1 . Further study will be needed to clarify the substance recognized by these monoclonal antibodies and its role in the mechanism of metastasis in this tumor.

犬恶性黑色素瘤具有生长迅速、局部侵袭范围广、转移早、化疗和放疗耐药、转移早等特点。在小鼠中，已知膜糖蛋白AD-1或cd63在原发病变中出现，但在转移病变中消失，因此可能在肿瘤转移机制中发挥作用。本研究旨在阐明AD-1在犬恶性黑色素瘤转移中的作用。在第一个实验中，从犬患者身上建立黑色素瘤细胞系。分别从口腔粘膜和皮肤中培养了4株具有不同增殖方式和可移植性的细胞系。利用其中一个细胞，生产AD-1单克隆抗体。结果，获得了两种抗体。其中一种只对黑色素瘤细胞起反应，而另一种对黑色素瘤和肥大细胞瘤细胞都起反应，这两种细胞已知含有AD-1。不幸的是，目前还没有证明这些抗体是否对AD-1具有特异性。这两种抗体用于手术获得的自发性犬黑色素瘤组织的组织化学。结果，前一种抗体对原发性或转移性病变有反应；在一些标本中，原发组织呈阳性，转移组织呈阴性，而在其他组织中，转移组织呈阳性，原发组织呈阴性。这可能表明该抗体识别一些可能与转移有关的物质，但可能不识别AD-1。需要进一步研究这些单克隆抗体识别的物质及其在该肿瘤转移机制中的作用。