Metabolism of tryptophan metabolites in human erythrocytes

人红细胞中色氨酸代谢物的代谢

• 批准号: 60570127
• 负责人: TOMODA Akio
• 金额: $ 1.02万
• 依托单位: Department of Biochemistry, Kanazawa University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570127/
• 关键词: Metabolism of tryptophan metabolites; 3-hydroxykynurenine; 3-hydroxyanthranilic acid; o-aminophenol

Metabolism of tryptophan and its metabolites is physiologically important in human body. The process of metabolism of tryptophan and its metabolites such as 3-hydroxykynurenine and 3-hydroxyanthranilic acid is known to be present in the cells of brain and kidney etc. However, there are few reports on the metabolism of these compounds in human erythrocytes. We investigated whether tryptophan and its metabolites are metabolized in human erythrocytes or not. Interestingly, we found that these compounds were metabolized to brown or yellow pigments such as cinnabarinic acid and xanthommatin in the cells. These processes included dimerization of 3-hydroxykynurenine and 3-hydroxyanthranilic acid by intracellular hemoglobin in human erythrocytes. In this research, the mechanism for metabolism of tryptophan metabolites such as 3-hydroxykynurenine and 3-hydroxyanthranilic acid was investigated extensively.1). 3-Hydroxykynurenine, a metabolite of tryptophan, was metabolized to xanthommatin, which … More is not usually found in human body, in human erythrocytes. The reaction rates were relatively fast in the cells. We are to investigate the accumulation of this brown pigment in the patients of Diabetes.2). 3-Hydroxyanthranilic acid, a metabolite of 3-hydroxykynurenine in human body, was metabolized to cinnabarinic acid, an orange colored compound, in human erythrocytes. The reaction rates were very fast. The physiological and pathological significance of the accumulation of cinnabarinic acid in human body is now under investigation.3). o-Aminophenol, which has basal structure of 3-hydroxykynurenine and 3-hydroxyanthranilic acid is metabolized to phenoxazine, a brown pigment, in human erythrocytes. Since o-aminophenol causes toxic methemoglobinemia, the accumulation of phenoxazine compound is conceivable in the bloods of the patient.4). It was found that the metabolism of 3-hydroxykynurenine, 3-hydroxyanthranilic acid and o-aminophenol is coupled with oxidation and reduction of intracellular hemoglobin in human erythrocytes.5). The brown or orange colored pigments such as xanthommatin, cinnabarinic acid and phenoxazine are not found in human body before we reported. Less

色氨酸及其代谢物在人体内的代谢具有重要的生理意义。色氨酸及其代谢物3-羟基犬尿氨酸和3-羟基邻氨基苯甲酸的代谢过程存在于脑、肾等细胞中，但有关这些化合物在人红细胞中代谢的报道较少。我们研究了色氨酸及其代谢物是否在人红细胞内代谢。有趣的是，我们发现这些化合物在细胞中被代谢成棕色或黄色的色素，如朱砂酸和黄褐素。这些过程包括人红细胞内血红蛋白对3-羟基犬尿氨酸和3-羟基邻氨基苯甲酸的二聚化。本研究对色氨酸代谢产物3-羟基犬尿氨酸和3-羟基邻氨基苯甲酸的代谢机理进行了深入的研究。色氨酸的代谢物3-羟基犬尿氨酸被代谢成黄褐素，…更多的是在人体内找不到的，在人的红细胞中。细胞内的反应速度相对较快。我们将调查这种棕色色素在糖尿病患者体内的蓄积情况。人体内3-羟基犬尿氨酸的代谢产物3-羟基邻氨基苯甲酸在人红细胞内被代谢成橙色化合物朱砂酸。反应速度非常快。目前正在研究朱砂酸在人体内蓄积的生理和病理意义。邻氨基苯酚具有3-羟基犬尿氨酸和3-羟基邻氨基苯甲酸的基本结构，在人红细胞中被代谢成棕色色素吩恶嗪。由于邻氨基苯酚会引起中毒性高铁血红蛋白血症，因此可以想象非诺嗪化合物在患者血液中的蓄积。研究发现，人红细胞内3-羟基犬尿氨酸、3-羟基邻氨基苯酚和3-羟基犬尿氨酸的代谢与细胞内血红蛋白的氧化还原相耦合。在我们报道之前，在人体内没有发现棕色或橙色的色素，如黄褐素、朱砂酸和吩恶嗪。较少

项目成果

山口順道,友田あき夫,米山良昌: 第13回国際生化学会記録集. (1985)

Jundo Yamaguchi、Akio Tomoda、Yoshimasa Yoneyama：第 13 届国际生物化学学会记录（1985）。

友田あき夫,米山良昌: HEMOGLOBIN. 10. 33-38 (1986)

友田昭夫，米山义正：血红蛋白。10. 33-38 (1986)

友田あき夫,山口順道,小島久則,米山良昌: FEBS Letters. 196. 44-48 (1986)

Akio Tomoda、Junmichi Yamaguchi、Hisanori Kojima、Yoshimasa Yoneyama：FEBS Letters。 196. 44-48 (1986)

友田あき夫,矢川克郎,米山良昌: Biomed.biochim.Acta. 68. (1987)

友田昭夫、矢川胜郎、米山吉正：Biomed.biochim.Acta 68。（1987）

友田あき夫,橋本琢磨,谷島清郎,松原藤継,米山良昌: Endocrinologica Japonica. 32. 753-757 (1985)

Akio Tomoda、Takuma Hashimoto、Kiyoro Tanijima、Fujitsugu Matsubara、Yoshimasa Yoneyama：《日本内分泌学》32. 753-757 (1985)。