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Study on Protective Immunity to Cestode Infection in Murine Hosts

小鼠宿主对绦虫感染的保护性免疫研究

基本信息

批准号：
60570181
负责人：
OKAMOTO Kenichi
金额：
$ 1.28万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570181/
关键词：
Protective immunity Passive transfer Hymenolepis nana Nude mouse; Mesenteric lymph node cells T-cells Lyt抗原IgA

项目摘要

Mice given a single oral infection with Hymenolepis nana eggs develop a rapid and strong acquired immunity as demonstrated by complete rejection of cysticercoid larvae in the intestinal villi. We already indicated that the immunity was passively transferable fo congenitally athymic nude mice (recipients) by mesenteric lymph node cells (MLNC) obtained from donor mice. The conclusions from the present study on passive transfer were as follows:1. Passive transfer of immune MLNC obtained from donor mice immunized 4 day before cell collection (4-day-immune MLNC) resulted in complete rejection of cysticercoids from the challenge in recipient nude mice.2. Immune MLNC obtained from donors immunized 10 or 21 days before cell collection (10- or 21-day-immune MLNC) and spleen cells obtained from donors immunized 4 days before cell collection, however, had no effect on the rejection of cysticercoids.3. It was also indicated that the immunity was passively transferable to recipients by T cells, especially by T cell subset of phenotype Lyt-1 but not those of phenotype Lyt-2.3 and Lyt-1.2.3.4. Non-effective 10-day-immune MLNC was suspended in culture medium RPMI-1640 and incubated with soluble H. nana egg extract, which was prepared in 0.05M PBS following ultrasonication, at 37゜C for 4 days. The treatment with egg extract increased the capacity of the cells to passively transfer strong immunity, i.e., almost all of the recipient mude mice received the treated MLNC were negative for cysticercoids. These treated cells also showed a marked increase in <^3H> -TdR incorporation (186% of control value).On the other hand, an attempt was made to establish a selfed strain of H. nana in mice by repetition of self-fertirization. H. nana can survive repeated selfing and up to the time writing has been maintained for at least 23 selfed generations.

用微小膜壳绦虫卵单次口服感染的小鼠产生快速和强烈的获得性免疫，如通过肠绒毛中的囊尾蚴幼虫的完全排斥所证明的。我们已经指出，免疫是被动转移给先天无胸腺裸鼠（受体）的肠系膜淋巴结细胞（MLNC）从供体小鼠获得。本研究的主要结论如下：1。将采集细胞前4天免疫的供体小鼠获得的免疫MLNC（4-day-immune MLNC）被动转移到受体裸鼠体内，导致对囊尾蚴攻击的完全排斥.采集细胞前10或21天免疫供体获得的免疫MLNC（10-或21-d-immune MLNC）和采集细胞前4天免疫供体获得的脾细胞对囊虫的排斥反应没有影响.免疫力可通过T细胞被动传递给受者，尤其是表型为Lyt-1的T细胞亚群，但表型为Lyt-2.3和Lyt-1.2.3.4的T细胞亚群不能传递。将10天免疫无效的MLNC悬浮于培养基RPMI-1640中，并与可溶性H.在37 ℃下超声处理4天后，在0.05M PBS中制备纳米卵提取物。鸡蛋提取物处理增加了细胞被动转移强免疫力的能力，即，几乎所有接受处理的MLNC的受体小鼠对类囊尾蚴呈阴性。这些处理细胞的<^3H> -TdR参入量也显著增加（对照值的186%）。通过重复自受精在小鼠中产生nana。H.娜娜可以在反复的自交中存活下来，并且直到至少23个自交世代都能保持书写。