Heterogeneity of Prolactin in Sera and CSF in Prolactinoma Patients

催乳素瘤患者血清和脑脊液中催乳素的异质性

• 批准号: 63570670
• 负责人: SAKAMOTO Tetsuya
• 金额: $ 1.28万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570670/
• 关键词: Prolactin; Prolactinoma; Radioimmunoassay; Radioreceptor assay; Bioassay; RIA; RRA; 下垂体腺腫; リンフォ-マ細胞; 生物学的活性; 免疫活性; 生物活性; リンフォーマ細胞

In order to study the heterogeneity of prolactin, sera and cerebrospinal fluid obtained from prolactinoma patients were studied employing gel chromatography, radioimmunoassay, radioreceptor assay and bioassay. Chromatography in sera and cerebrospinal fluid gave three types of prolactin with different molecular weights. Radioreceptor assay was used by using a receptor propagation from bovine ovaries. Bioassay was used by using the rat Nb_2 lymphoma cell line. In sera, there was a good correlation between receptor assay, bioassay and radioimmunoassay.

为了研究催乳素的异质性，采用凝胶层析法、放射免疫法、放射受体测定法和生物测定法对催乳素瘤患者的血清和脑脊液进行了研究。血清和脑脊液的层析得到三种不同相对分子质量的催乳素。放射受体分析是利用牛卵巢的受体繁殖。用大鼠NB2淋巴瘤细胞系进行生物测定。在血清中，受体法、生物法和放射免疫法之间有很好的相关性。

项目成果

坂本 哲也,他: "プロラクチンレセプタ-から見た難治性プロラクチノ-マ" 秋田医学.

Tetsuya Sakamoto 等：“从催乳素受体的角度看难治性催乳素瘤”秋田医学。