The effect of sperm concentration on the incidence of polyspermy and chromosomal analysis of rabbit embryos fertilized in vitro.

精子浓度对多精发生率的影响及体外受精兔胚胎染色体分析。

• 批准号: 60570764
• 负责人: ASAKAWA Takehito
• 金额: $ 0.83万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570764/
• 关键词: Chromosome; Polyspermy; In vitro fertilization

1. In chromosomal studies, 94 (77.7%) of 121 oocytes fertilized in vitro had a normal diploid complement of chromosomes (2n=44) and 6 (5%) showed aneuploidy. The incidense of triploid (dispermy) after in vitro fertilization was higher than the rate in vivo (16.3% V.S. 9.0%).2. The analysis of in vitro fertilized rabbit embryos reavealed that the incidence of polyploydy (polyspermy) was not effected on by PMS 100 i.u. given to the female.3. The mucin layer surrounding the rabbit oocytes was not effective to block the polyspermic fertilization in vitro.4. A 100 fold range of capacitated sperm concentrations ( <10^4> , <10^5> , <10^6> /ml) has been tested to determine whether there is a relationship between the capacitated sperm concentration and the the incidence of polyspermic fertilization in vitro of rabbit oocytes. The sperm concentration in the range of <10^4> - <10^6> /ml gave fertilization rate of around 70%. The frequency of polyploidy (polyspermy) was dependent on the sperm concentration. The rate of polyspermy with <10^4> /ml spermatozoa was the lowerest (9.3%), compared to 14.1% with <10^5> /ml and 13.2% with <10^6> /ml.

1.在染色体研究中，94（77.7%）的121个卵母细胞在体外受精有一个正常的二倍体染色体（2n=44）和6（5%）显示非整倍体。体外受精后三倍体（双二倍体）的发生率高于体内受精（16. 3% vs. 9.0%）。对兔体外受精胚胎的分析表明，PMS 100 i.u.给女性的。兔卵母细胞周围的粘蛋白层不能有效地阻断多精受精.本实验测定了100倍获能精子浓度（<10^4>、<10^5>、<10^6> /ml），以确定获能精子浓度与兔卵母细胞体外多精受精发生率之间是否存在关系。精子浓度在<10^4> - <10^6> /ml范围内，受精率可达70%左右。多倍体（多精受精）的频率取决于精子浓度。精子数<10^4> /ml的多精受精率最低（9.3%），<10^5> /ml的多精受精率为14.1%，<10^6> /ml的多精受精率为13.2%。

项目成果

H.Takada, T.Asakawa, M.Ishikawa and T.Shimizu: "Effects of cryoprotectants and sucrose dilution on the survival of unfertilized and fertilized mouse eggs after freezing and thawing." Japanese Journal of Fertility and Sterility. 31. 187 (1986)

H.Takada、T.Asakawa、M.Ishikawa 和 T.Shimizu：“冷冻保护剂和蔗糖稀释对冷冻和解冻后未受精和受精小鼠卵存活的影响”。

高田久士,浅川竹仁,石川睦男,清水哲也: 日本不妊学会誌. 31. 187 (1986)

Hisashi Takada、Takehito Asakawa、Mutsuo Ishikawa、Tetsuya Shimizu：日本不孕症协会杂志 31. 187 (1986)。

T. Asakawa et. al.: "Chromosomal analysis after in vitro fertilization of Rabbit (Japanese White) oocytes." Acta Obstetrica et Gynaecologyca Japonica.37. 2614 (1985)

T.浅川等。