Studies on the control mechanism of menstrual cyclicity from the point of existence type of estrogen in blood

在血液中检测到一种不同于睾酮-雌二醇结合球蛋白(TeBG)的高亲和力、低容量的血清雌二醇结合蛋白(&lt；E_2&gt；BP)。以己烯雌酚为竞争对手，用&lt；测定&lt；E_2&GT；BP-BC的样品用35%的硫酸铵制粒。测定了正常月经周期的TeBG、无生理性E_2&GT和总E_2&GT；在&lt；E_2&Gt；BP-BC值上，在正常周期内观察到周期性变化。排卵前期最大结合容量为3~5 nM。在正常月经周期中，结合容量的值似乎与总E_2和E_2水平平行。在整个月经周期中，~lt；E_2&gt；的生理游离部分保持相对恒定。在正常周期中，约有30%的卵泡液中有生理游离态，在卵泡液中检测到少量的&lt；E_2&Gt；-BP，但经Scatchard分析其解离常数为0.45。等电聚焦分析显示低亲和力和高亲和力两种成分，提示雌激素及其结合蛋白在控制女性月经周期中起重要作用。应进一步检查以阐明结合蛋白的作用。

A High affinity, low capacity serum estradiol binding protein( <E_2> Bp) which is distinct from testosterone-estradiol binding globulin(TeBG) was detected in blood.Estradiol binding protein binding capacity( <E_2> BP-BC) in 10 normal menstrual cycles was measured by <^3H> - <E_2> binding analysis using diethylstilbestrol as a competitor steroid. Sample preparation for measurement of <E_2> Bp-BC was undertaken by pelleting with 35% ammonium sulfate. Also, TeBG, physiologically free <E_2> and total <E_2> levels were determined.The values of TeBG-BC were 50 to 60 nM during the normal menstrual cycle. No cyclic changes were observed.On the values of <E_2> Bp-BC, cyclic changes were observed during the normal cycle. The values of maximum binding capacity were 3 to 5 nM in the preovulatory phase. It appears that the values of binding capacity run parallel with the total <E_2> levels during the normal menstrual cycle. The physiologically free fractions of <E_2> remained relatively constant throughout the menstrual cycle. About 30% of total <E_2> was physiologically free during the normal cycle.In the follicular fluid, <E_2> -Bp was detected in small amount, but its Kd was 0.45 by Scatchard's analysis. Isoelectrofocusing analysis showed two component of low and high affinity.These findings suggested that estrogen and its binding protein must play an important role to control menstrual cyclicity in female. Further examination should be taken to elucidate the role of binding protein.