Studies on the control mechanism of menstrual cyclicity from the point of existence type of estrogen in blood

从血液中雌激素存在类型研究月经周期的控制机制

• 批准号: 60570767
• 负责人: HIROI Masahiko
• 金额: $ 1.28万
• 依托单位: YAMAGATA UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570767/
• 关键词: Estradiol binding protein( <E_2> -Bp); Testosterone-estradiol binding globulin(Te-BG); Estradiol; 性周期

A High affinity, low capacity serum estradiol binding protein( <E_2> Bp) which is distinct from testosterone-estradiol binding globulin(TeBG) was detected in blood.Estradiol binding protein binding capacity( <E_2> BP-BC) in 10 normal menstrual cycles was measured by <^3H> - <E_2> binding analysis using diethylstilbestrol as a competitor steroid. Sample preparation for measurement of <E_2> Bp-BC was undertaken by pelleting with 35% ammonium sulfate. Also, TeBG, physiologically free <E_2> and total <E_2> levels were determined.The values of TeBG-BC were 50 to 60 nM during the normal menstrual cycle. No cyclic changes were observed.On the values of <E_2> Bp-BC, cyclic changes were observed during the normal cycle. The values of maximum binding capacity were 3 to 5 nM in the preovulatory phase. It appears that the values of binding capacity run parallel with the total <E_2> levels during the normal menstrual cycle. The physiologically free fractions of <E_2> remained relatively constant throughout the menstrual cycle. About 30% of total <E_2> was physiologically free during the normal cycle.In the follicular fluid, <E_2> -Bp was detected in small amount, but its Kd was 0.45 by Scatchard's analysis. Isoelectrofocusing analysis showed two component of low and high affinity.These findings suggested that estrogen and its binding protein must play an important role to control menstrual cyclicity in female. Further examination should be taken to elucidate the role of binding protein.

用<E_2><E_2>~ 3 H结合分析法测定了10个正常月经周期妇女血清雌二醇结合蛋白结合容量（BP-BC）<E_2>。通过<E_2>用35%硫酸铵造粒来进行用于测量Bp-BC的样品制备。此外，TeBG，生理游离<E_2>和总<E_2>水平进行了测定。TeBG-BC的值为50至60 nM在正常的月经周期。Bp-BC值<E_2>在正常月经周期内呈周期性变化。在排卵前期，最大结合容量的值为3至5 nM。在正常月经周期中，结合能力的值似乎与总水平平行<E_2>。在<E_2>整个月经周期中，生理游离分数保持相对恒定。在<E_2>正常月经周期中，约有30%的-Bp是游离的，在卵泡液中<E_2>检测到少量的-Bp，Scatchard分析其Kd值为0.45。等电聚焦分析显示雌激素及其结合蛋白具有高亲和力和低亲和力两种成分，提示雌激素及其结合蛋白在调节女性月经周期中起重要作用。结合蛋白的作用有待进一步研究。