Visualization of the microtubules in a mitotic apparatus and study of the mechanism of chromosome movement.

有丝分裂装置中微管的可视化和染色体运动机制的研究。

• 批准号: 60580209
• 负责人: HOTANI Hirokazu
• 金额: $ 1.41万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60580209/
• 关键词: Microtubule; Dynamic instability; MAPs; Dark-field light microscopy; 微小管結合タンパク質; 暗視野光学顕微鏡; 分裂装置

Chromosome movement is performed by aid of microtubule filaments in a mitotic spindle, so that the studies on the dynamic hehabiour of microtubules are closely related to the mechanism of the chormosome movement. I visualized individual microtubules in vitro by high intensity dark-field light microscopy. Real-time video recording reveales the following evidences.1) Two populations of microtubules coexist in a dynamically unstable manner in vitro: those in one population elongate while those in the other shorten. 2) Both ends of a microtubule exist in either the growing or the shortening phase and alternate guite frequently between the two phases in a stochastic manner. 3) Growing and shortening ends can coexist even on a single microtubule, one end continuing to grow simultaneously with shortening at the other end. 4) There is no correlation in the phase conversion either among individual microtubules or between the two ends of a single microtubule. 5) The two ends of any given microtubule have remarkably different charactrstics; the active end grows faster, alternates in phase more frequently and fluctuates in length to a greater extent than the inactive end. 6) Microtubule-associated proteins suppress the phase conversion and stabilize microtubules in the growing phase.

染色体的运动是通过有丝分裂纺锤体中的微管细丝完成的，因此对微管动态行为的研究与染色体运动的机制密切相关。我在体外用高强度暗视野光学显微镜观察了单个微管。实时视频记录揭示了以下证据：1)两个微管种群在体外以动态不稳定的方式共存：一个种群中的微管延长，另一个种群中的微管缩短。2)微管的两端或处于生长阶段，或处于缩短阶段，并以随机方式在两个阶段之间频繁交替。3)即使在单个微管上，生长和缩短的末端也可以共存，一端继续生长，另一端缩短。4)单个微管之间和单个微管两端之间的相变没有相关性。5)任何给定微管的两端都具有明显不同的特征：活跃端生长较快，同相交替较频繁，长度波动幅度较大。6)在生长期，微管相关蛋白抑制微管的相变，稳定微管。

项目成果

宝谷紘一,堀尾哲也: 遺伝. 40. 21-27 (1986)

宝谷浩一、堀尾哲也：遗传学 40. 21-27 (1986)

H. Hotani: Sozo Co. Ltd.Developement of Biophysics, 172 (1986)

H. Hotani：Sozo Co. Ltd. 生物物理学的发展，172 (1986)

T.Horio;H.Hotani: Nature. 321. 605-607 (1986)

T.Horio；H.Hotani：自然。

H.Hotani;T.Horio;Joel L.Rosenbaum: J.Cell Biol.(1987)

H.Hotani；T.Horio；Joel L.Rosenbaum：J.Cell Biol.(1987)

T. Horio & H. Hotani: "Visualization of the dynamic instability of individual microtubules by dark-field microscopy." Nature. 321. 605-607 (1986)

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