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Electron Microscopic Study on the Vitamin A-Storing Cells

维生素 A 储存细胞的电子显微镜研究

基本信息

批准号：
61440023
负责人：
HIROSAWA Kazushige
金额：
$ 21.44万
依托单位：
The University of Tokyo, The Institute of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Vitamin A (VA), not synthesized by animals, is stored in the animal body. A main depot has been considered to be hepatic fat-storing cells, but in the case of hypervitaminosis A, it is also stored in fibroblasts of the lung, adrenal, gastrointestinal tract, et. We have defined these cells as "vitamin A-storing cell system". First of all, this working hypothesis is summarized in a paper titled " an introduction of vitamin A-storing cell system" in a Japanese journal. This topics has become international, and a symposium was held at Kyoto, Septemter 1986. Histological demonstration of VA-storing cells was further carried out on the liver of Watase threw. Though the cell number was smaller than that of ordinary laboratory animals, fat-storing cells were identified in the liver. (presented at 92nd annual meeting of Anatomists). In vivo and in vitro studies on the VA uptake by fibroblasts have been continued. An in vivo study of villous fibroblasts was done on the Suncus small intestine. We … More identified two types of fibroblast in the intestinal lamina propria; one was ^3H-VA labeled fibroblasts and the other was non-labeled ones. Serial sections were examined throughly, and a conclusion was reached that the non-labeled cells had no lipid droplets at all. Labeled cells were localized in the middle of lamina propria, while non-labeled ones were found beneath the basal lamina of epithelium. Serial sections of both types of cells were reconstructed three dimensionally with an image analyser, JIM 5000. This results will be published in the J. Electron Microscopy. Dissociated fat-storing cells were primary-cultured for several days and were administered with ^3H-VA in the culture medium. It was confirmed that cultured fibroblasts incorporated VA. The result was published in the "Hepatology" (Japanese text). Cutaneous fibroblasts were cultured and various concentrations of VA acetate were added. Even at a physiological concentration, in vitro fibroblasts incorporated VA into cytoplasmic lipid droplets. HPLC analysis revealed that the stored VA were palmitic and oleic esters. As the concentration increased, various non-physiological VA esters were found in lipid droplets, suggesting that VA metabolism was disturbed in the cytoplasm. The result will be published in the Exp. Cell Rex. Less

维生素A（VA）不是由动物合成的，储存在动物体内。一个主要的贮库被认为是肝脂肪储存细胞，但在维生素A过多症的情况下，它也储存在肺、肾上腺、胃肠道等的成纤维细胞中。我们将这些细胞定义为“维生素A储存细胞系统”。首先，这一工作假设在一篇题为“维生素A储存细胞系统介绍”的论文中进行了总结，该论文发表在日本期刊上。这一主题已成为国际性的，1986年9月在京都举行了一次专题讨论会。在Watase throw的肝脏上进一步进行VA储存细胞的组织学展示。虽然细胞数量比普通实验室动物少，但在肝脏中发现了储存脂肪的细胞。（出席第92届解剖学家年会）。成纤维细胞对VA摄取的体内和体外研究仍在继续。绒毛成纤维细胞的体内研究在Suncus小肠上进行。我们 ...更多信息在肠固有层中鉴定出两种类型的成纤维细胞，一种是^3 H-VA标记的成纤维细胞，另一种是未标记的成纤维细胞。对连续切片进行了全面检查，得出的结论是，未标记的细胞根本没有脂滴。标记细胞位于固有层中部，未标记细胞位于上皮基底层下方。用JIM 5000图像分析仪对两种细胞的连续切片进行三维重建。这一结果将发表在J. Electron Microscopy上。将分离的贮脂细胞原代培养数天，并在培养基中加入^3 H-VA。证实培养的成纤维细胞掺入VA。结果发表在《肝病学》（日文版）上。培养皮肤成纤维细胞并加入不同浓度的VA醋酸盐。即使在生理浓度下，体外成纤维细胞将VA掺入细胞质脂滴中。HPLC分析表明，储存的VA是棕榈酸酯和油酸酯。随着浓度的增加，在脂滴中发现了各种非生理性的VA酯，表明细胞质中的VA代谢受到干扰。结果将在Exp.牢房雷克斯。少