Electron Microscopic Study on the Vitamin A-Storing Cells

维生素A （VA）不是由动物合成的，而是储存在动物体内。我们认为主要的储存点是肝脏脂肪储存细胞，但在维生素A过多症的情况下，它也储存在肺、肾上腺、胃肠道等成纤维细胞中。我们将这些细胞定义为“维生素A储存细胞系统”。首先，这一工作假设在日本杂志上的一篇题为“维生素a储存细胞系统的介绍”的论文中得到了总结。这个题目已成为国际性的，1986年9月在京都举行了一次专题讨论会。在肝上进一步进行va储存细胞的组织学证明。虽然细胞数量比普通实验动物少，但在肝脏中发现了脂肪储存细胞。（发表于第92届解剖学家年会上）。成纤维细胞对VA摄取的体内和体外研究仍在继续。在猕猴小肠上进行了绒毛成纤维细胞的体内研究。我们…More在肠固有层发现了两种类型的成纤维细胞；一组为^3H-VA标记成纤维细胞，另一组为未标记成纤维细胞。对连续切片进行了彻底的检查，得出结论：未标记的细胞完全没有脂滴。标记细胞位于固有层中间，未标记细胞位于上皮基底层下方。用图像分析仪JIM 5000对两种细胞的连续切片进行三维重建。这一结果将发表在《电子显微镜》杂志上。分离的脂肪储存细胞原代培养数天，并在培养基中加入^3H-VA。证实培养的成纤维细胞含有VA，结果发表在《肝脏学》（日文）上。培养皮肤成纤维细胞，加入不同浓度的醋酸VA。即使在生理浓度下，体外成纤维细胞也将VA纳入细胞质脂滴。高效液相色谱分析表明，所储存的VA为棕榈酯和油酯。随着浓度的增加，脂滴中出现了各种非生生性的VA酯，表明细胞质中VA代谢受到干扰。结果将发表在Exp. Cell Rex上。少

Vitamin A (VA), not synthesized by animals, is stored in the animal body. A main depot has been considered to be hepatic fat-storing cells, but in the case of hypervitaminosis A, it is also stored in fibroblasts of the lung, adrenal, gastrointestinal tract, et. We have defined these cells as "vitamin A-storing cell system". First of all, this working hypothesis is summarized in a paper titled " an introduction of vitamin A-storing cell system" in a Japanese journal. This topics has become international, and a symposium was held at Kyoto, Septemter 1986. Histological demonstration of VA-storing cells was further carried out on the liver of Watase threw. Though the cell number was smaller than that of ordinary laboratory animals, fat-storing cells were identified in the liver. (presented at 92nd annual meeting of Anatomists). In vivo and in vitro studies on the VA uptake by fibroblasts have been continued. An in vivo study of villous fibroblasts was done on the Suncus small intestine. We … More identified two types of fibroblast in the intestinal lamina propria; one was ^3H-VA labeled fibroblasts and the other was non-labeled ones. Serial sections were examined throughly, and a conclusion was reached that the non-labeled cells had no lipid droplets at all. Labeled cells were localized in the middle of lamina propria, while non-labeled ones were found beneath the basal lamina of epithelium. Serial sections of both types of cells were reconstructed three dimensionally with an image analyser, JIM 5000. This results will be published in the J. Electron Microscopy. Dissociated fat-storing cells were primary-cultured for several days and were administered with ^3H-VA in the culture medium. It was confirmed that cultured fibroblasts incorporated VA. The result was published in the "Hepatology" (Japanese text). Cutaneous fibroblasts were cultured and various concentrations of VA acetate were added. Even at a physiological concentration, in vitro fibroblasts incorporated VA into cytoplasmic lipid droplets. HPLC analysis revealed that the stored VA were palmitic and oleic esters. As the concentration increased, various non-physiological VA esters were found in lipid droplets, suggesting that VA metabolism was disturbed in the cytoplasm. The result will be published in the Exp. Cell Rex. Less