Structural specificities ofiris tissues in the vertebrate eye

1. 鸡虹膜和睫状体单克隆抗体：从鸡眼球中分离出虹膜和睫状体及其附属物，根据本实验室开发的方法（参考Exp eye Res 67,1998）进行单克隆抗体的制备。用于研究的小鼠体内产生了多种抗体。染色模式分为14种。所得抗体中，9个抗体被建立为单克隆抗体。他们的特征正在被分析。虹膜血管概述：虹膜间质中可见小动脉、小静脉和毛细血管。其中，毛细血管是最容易识别和经常遇到的。毛细血管有未出肠的内皮细胞，内皮细胞之间紧密连接。毛细血管内皮细胞被厚的基底层和厚的胶原纤维包围。关于猴睫状肌和虹膜内皮细胞的通透性：睫状肌的毛细血管内皮细胞在结构上不同于睫状肌的毛细血管内皮细胞，它们是不出肠的。从这个角度出发，我们用纤毛肌和虹膜内皮细胞研究了马萝卜过氧化物酶（HRP）对未出肠内皮细胞的通透性。简单地说，HRP注入前房后，在15分钟内进入虹膜间质、内皮细胞细胞体和毛细血管管腔。相反，注入静脉系统的HRP在30min后仍停留在毛细血管管腔和细胞体内。这一结果与迄今为止报告的结果一致。

1. Monoclonal antibodies against chick iris and ciliary body :Iris and ciliary bodies with their appendages were dissected from chick eye balls and were processed for rainsing of monoclonal antibody according to the method developed in our laboratory (ref. Exp Eye Res 67,1998).A variety of antibodies were produced in the mice used for the study. Staining patterns were categorized into 14. Among the antibodies raised, 9 antibodies were established as monoclonal antibodies. Their characterisation is being analyzed.2. Overview of vessels in the iris :Arterioles, venules and capillaries were found in the iris stroma. Among them, capillaries were most easily identified and frequently encountered. The capillaries had unfenestrated endothelial cells, between which were sealed with tight junctions. Capillary endothelial cells were surrounded with thick basal lamina and thick collagen fibriles.3. On the permeability of endothelial cells in ciliary muscles and iris of monkey :Capillary endothelial cells in the ciliary muscles are structually different from those of the ciliary processes in that they are unfenestrated. From this point of view, both ciliary muscles and iris endothelial cells were used to study the permeability of horse radish peroxidase(HRP)to the unfenestrated endothelial cells. In brief, HRP injected into the anterior chamber entered the iris stroma, cell bodies of endothelial cells and into the lumen of capillaries in 15 min. On the contrary, HRP injected into the venular system stayed in the capillary lumen and the cell bodies even after 30min. This result is consistent with results reported so far.