Structural specificities ofiris tissues in the vertebrate eye

脊椎动物眼睛虹膜组织的结构特异性

• 批准号: 09470003
• 负责人: HIROSAWA Kazushige
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470003/
• 关键词: Iris; Monoclonal Antibody; Pigment Cell; Capillary; HRP

1. Monoclonal antibodies against chick iris and ciliary body :Iris and ciliary bodies with their appendages were dissected from chick eye balls and were processed for rainsing of monoclonal antibody according to the method developed in our laboratory (ref. Exp Eye Res 67,1998).A variety of antibodies were produced in the mice used for the study. Staining patterns were categorized into 14. Among the antibodies raised, 9 antibodies were established as monoclonal antibodies. Their characterisation is being analyzed.2. Overview of vessels in the iris :Arterioles, venules and capillaries were found in the iris stroma. Among them, capillaries were most easily identified and frequently encountered. The capillaries had unfenestrated endothelial cells, between which were sealed with tight junctions. Capillary endothelial cells were surrounded with thick basal lamina and thick collagen fibriles.3. On the permeability of endothelial cells in ciliary muscles and iris of monkey :Capillary endothelial cells in the ciliary muscles are structually different from those of the ciliary processes in that they are unfenestrated. From this point of view, both ciliary muscles and iris endothelial cells were used to study the permeability of horse radish peroxidase(HRP)to the unfenestrated endothelial cells. In brief, HRP injected into the anterior chamber entered the iris stroma, cell bodies of endothelial cells and into the lumen of capillaries in 15 min. On the contrary, HRP injected into the venular system stayed in the capillary lumen and the cell bodies even after 30min. This result is consistent with results reported so far.

1.抗鸡虹膜和睫状体的单克隆抗体：从鸡眼球中分离虹膜和睫状体及其附属物，按照本实验室开发的方法（参考文献Exp Eye Res 67，1998）进行单克隆抗体的制备。染色模式分为14种。在所产生的抗体中，9个抗体被确定为单克隆抗体。分析了它们的特征。虹膜血管概况：虹膜基质中可见小动脉、小静脉和毛细血管。其中，毛细血管最容易识别，也是最常见的。毛细血管有无窗孔的内皮细胞，它们之间由紧密连接密封。毛细血管内皮细胞被较厚的基膜和胶原纤维所包围.关于猴睫状肌和虹膜内皮细胞通透性的研究：睫状肌和睫状突的毛细血管内皮细胞结构不同，它们是无孔的。从这个角度出发，我们用睫状肌和虹膜内皮细胞来研究辣根过氧化物酶（HRP）对无孔内皮细胞的通透性。总之，前房注射HRP 15min后即进入虹膜基质、内皮细胞胞体及毛细血管腔，而微静脉系统注射HRP 30min后仍停留在毛细血管腔及胞体内。这一结果与迄今为止报告的结果一致。