Structural specificities ofiris tissues in the vertebrate eye

1.抗鸡虹膜和睫状体的单抗：取鸡眼球的虹膜和睫状体及其附属物，按本实验室建立的方法进行单抗沉淀。在用于研究的小鼠中产生了各种抗体。共分14种染色模式，其中9种为单抗。他们的特征正在分析中。虹膜血管概况：虹膜间质内可见小动脉、小静脉和毛细血管。其中，毛细血管最容易辨认，也是最常见的。毛细血管有未开窗的内皮细胞，它们之间用紧密的连接密封。毛细血管内皮细胞周围有较厚的基底膜和较厚的胶原纤维。关于猕猴睫状肌和虹膜内皮细胞的通透性：睫状肌中的毛细血管内皮细胞与睫状突的毛细血管内皮细胞在结构上不同，因为它们没有开窗。从这一角度出发，利用睫状肌和虹膜内皮细胞研究了辣根过氧化物酶(HRP)对未开窗内皮细胞的通透性。总之，前房注射HRP在15min内进入虹膜间质、内皮细胞胞体和毛细血管腔。相反，注射到静脉系统中的HRP即使在30min后仍停留在毛细血管腔和细胞体中。这一结果与迄今报道的结果一致。

1. Monoclonal antibodies against chick iris and ciliary body :Iris and ciliary bodies with their appendages were dissected from chick eye balls and were processed for rainsing of monoclonal antibody according to the method developed in our laboratory (ref. Exp Eye Res 67,1998).A variety of antibodies were produced in the mice used for the study. Staining patterns were categorized into 14. Among the antibodies raised, 9 antibodies were established as monoclonal antibodies. Their characterisation is being analyzed.2. Overview of vessels in the iris :Arterioles, venules and capillaries were found in the iris stroma. Among them, capillaries were most easily identified and frequently encountered. The capillaries had unfenestrated endothelial cells, between which were sealed with tight junctions. Capillary endothelial cells were surrounded with thick basal lamina and thick collagen fibriles.3. On the permeability of endothelial cells in ciliary muscles and iris of monkey :Capillary endothelial cells in the ciliary muscles are structually different from those of the ciliary processes in that they are unfenestrated. From this point of view, both ciliary muscles and iris endothelial cells were used to study the permeability of horse radish peroxidase(HRP)to the unfenestrated endothelial cells. In brief, HRP injected into the anterior chamber entered the iris stroma, cell bodies of endothelial cells and into the lumen of capillaries in 15 min. On the contrary, HRP injected into the venular system stayed in the capillary lumen and the cell bodies even after 30min. This result is consistent with results reported so far.