Rapid clonal multiplication of three Lilium species cultured in vitro

体外培养的三种百合属物种的快速克隆增殖

• 批准号: 61560029
• 负责人: NIIMI Yoshiji
• 金额: $ 1.22万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560029/
• 关键词: L.rubellum; L.japonicum; L.nobilissimum; Leaf segment; 鱗片分割培養; 子球培養; 開花球; 葉片培養; 鱗片切片培養; 固形培地培養; 液体振とう培地培養

The present study was made to establish a tissue culture method for rapid clonal multiplication of Lilium rubellum Baker, L. japonicum and L. nobilissimum. Murashige and Skoog's inorganic nutrient medium(1962)(MS-medium) supplemented with vitamins and growth regulators(NAA,BA or both) was used. The transplantation to soil of the in vitro propagated bulblets was also described.(1) Segments of growing leaves of L. japonicum and L. nobilissimum as well as those of scales developed bulblets well. The optimum concentrations of growth regulators NAA and BA in culturing the leaf segments were different: 0.5mg/l NAA and 0.05mg/l BA stimulated the induction and development of bulblets in L. japonicum; and 1.0mg/l NAA and 0.05mg/l BA in L. nobilissimum. In addition, these explants were not contaminted, unlike scale segments. Thus, it can be concluded that the leaf segments were superior to those of scales for the propagation of bulblets.(2) To improve the rate of bulblet regeneration in the leaf … More segment cultures of L. rubellum, they were precultured in liquid MS-medium for more than 24h and then cultured in MS-medium solidfied with agar. But, the preculturing was found to be ineffecitve for otaining an increased rate of regeneration. The same method was used in the scale segment cultures of three lily plants to perform the mass propagation, but it was ineffective as well. Although a few other methods were examined for increasing the number of bulblets, the establishment of general methods for this has been unsuccessful so for.(3) When bulblets formed on the explants of leaves, scales and stems were isolated and recultured in a fresh medium, they developed rapidly. Therefore, to obtain larger bulblets in a short period, it is necessary to isolate the developing bulblets from the explants and to reculture them as soon as possible.(4) The transplanting to soil of L. Japonicum and L. nobilissimum bulblets developed in vitro was investigated in relation to their dormancy. Leaf emergence from the bulblets was inhibited after transplanting into soil without previous low-temperature treatments. To break dormancy, low temperature treatment(4゜C) for at least more than 8 weeks was required in these bulblets. GAs treatment was also effective to break the dormancy of L. nobilissimum and resulted in reducing the periods necessary for the breaking of dormancy. Less

建立了一种快速无性系繁殖的百合、日本百合和新百合组织培养方法。使用Murashige和Skoog的无机营养培养基(1962)(MS-Medium)，添加维生素和生长调节剂(NAA、BA或两者都有)。本文还介绍了试管苗移栽到土壤中的情况。(1)日本狼尾草和新银杏的生长叶片和鳞片的小鳞茎发育良好。叶片培养中，NAA和BA的最适浓度不同：0.5 mg/L NAA和0.05 mg/L BA促进日本狼尾兰小鳞茎的诱导和发育，1.0 mg/L NAA和0.05 mg/L BA促进新麦草小鳞茎的诱导和发育。此外，与鳞片不同的是，这些外植体没有受到污染。由此可见，叶片切段对小鳞茎的繁殖效果优于鳞片。(2)提高叶片…的小鳞茎再生率在液体MS-培养基中预培养24小时以上，然后在MS-琼脂固化液中培养。但是，预培养对获得更高的再生率无效。用同样的方法在3株百合的鳞片段培养上进行了大量繁殖，但效果也不理想。虽然研究了一些其他方法来增加小鳞茎的数量，但建立通用的方法一直没有成功。(3)将叶片、鳞片和茎的外植体上形成的小鳞茎分离并在新鲜的培养基中再生时，小鳞茎发育迅速。因此，为了在短时间内获得更大的小鳞茎，有必要将发育中的小鳞茎从外植体中分离出来，并尽快进行再培养。(4)研究了小鳞茎移栽到土壤中与休眠的关系。未经低温处理的小鳞茎移栽入土壤后，小鳞茎出叶受到抑制。为了打破休眠，这些鳞茎需要进行至少8周以上的低温处理(4゜C)。气体处理也能有效地打破冬眠，缩短解除休眠所需的时间。较少