Rapid clonal multiplication of three Lilium species cultured in vitro

目前的研究是为了建立一种用于Lilium Rubellum Baker， L.的快速克隆复合材料的组织培养方法。日本和L.。nobilissimum。Murashige和Skoog的非有机营养介质（1962年）（MS-介质）补充了维生素和生长调节剂（NAA、BA或两者）。移植到体外植入的气泡中的土壤也被描述为“。（1） Segments of growing leaves of L.日本和L.。nobilissimum如well，即那些尺度开发的bulblets well。生长调节器NAA和BA in culturing the leaf segments were different：0.5 mg/l NAA和0.05 mg/l BA刺激的诱导和发展。japonicum;和1.0 mg/l NAA和0.05 mg/l BA in L. nobilissimum。在添加中，这些实例不受限制，不像刻度段。因此，它可以被包含在叶子段中，因为它们的大小是气泡的传播的最大值。（2）提高泡沫再生的速率在叶子上 ... More L.的部分文化。Rubellum，他们在24小时以上的液体中预先培养了MS-介质，然后在固体中培养了MS-介质。然而，由于再生率的增加，这种预培养被发现是不起作用的。同样的方法是在三棵百合植物的尺度段文化中使用的，以完成质量传播，但它是无效的。还有几种其他方法已经被测试了，以增加灯泡的数量，并为这已经是如此不成功的一般方法建立了。（3）当气泡形成在叶子、尺度和茎的样本上时，它们迅速发展起来。因此，要在短时间内制造更大的气泡，就有必要将从种植体中分离出来，并尽快将它们培养成可能的气泡。（4）移植到L的土壤。Japonicum and L.在体外开发的Nobilissimum Bulblets是在与他们的资助关系中进行调查的。气泡中的叶子生长在没有先前低温度处理的情况下移植到土壤后被抑制。为了休息，温度低的治疗（4 ° C），至少需要8周以上的时间。天然气治疗也是有效的，可以打破L的治疗。nobilissimum and resulted在减少周期的必要性中，以减少周期性的中断。Less（低）

The present study was made to establish a tissue culture method for rapid clonal multiplication of Lilium rubellum Baker, L. japonicum and L. nobilissimum. Murashige and Skoog's inorganic nutrient medium(1962)(MS-medium) supplemented with vitamins and growth regulators(NAA,BA or both) was used. The transplantation to soil of the in vitro propagated bulblets was also described.(1) Segments of growing leaves of L. japonicum and L. nobilissimum as well as those of scales developed bulblets well. The optimum concentrations of growth regulators NAA and BA in culturing the leaf segments were different: 0.5mg/l NAA and 0.05mg/l BA stimulated the induction and development of bulblets in L. japonicum; and 1.0mg/l NAA and 0.05mg/l BA in L. nobilissimum. In addition, these explants were not contaminted, unlike scale segments. Thus, it can be concluded that the leaf segments were superior to those of scales for the propagation of bulblets.(2) To improve the rate of bulblet regeneration in the leaf … More segment cultures of L. rubellum, they were precultured in liquid MS-medium for more than 24h and then cultured in MS-medium solidfied with agar. But, the preculturing was found to be ineffecitve for otaining an increased rate of regeneration. The same method was used in the scale segment cultures of three lily plants to perform the mass propagation, but it was ineffective as well. Although a few other methods were examined for increasing the number of bulblets, the establishment of general methods for this has been unsuccessful so for.(3) When bulblets formed on the explants of leaves, scales and stems were isolated and recultured in a fresh medium, they developed rapidly. Therefore, to obtain larger bulblets in a short period, it is necessary to isolate the developing bulblets from the explants and to reculture them as soon as possible.(4) The transplanting to soil of L. Japonicum and L. nobilissimum bulblets developed in vitro was investigated in relation to their dormancy. Leaf emergence from the bulblets was inhibited after transplanting into soil without previous low-temperature treatments. To break dormancy, low temperature treatment(4゜C) for at least more than 8 weeks was required in these bulblets. GAs treatment was also effective to break the dormancy of L. nobilissimum and resulted in reducing the periods necessary for the breaking of dormancy. Less