Studies on the yeast cell wall mutants.

酵母细胞壁突变体的研究。

• 批准号: 61560080
• 负责人: NAKAJIMA Tasuku
• 金额: $ 1.15万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560080/
• 关键词: Yeast; Cell Wall; Yeast mutants; Single cell protein; <beta>-glucan; 細胞壁変異株

Yeast cell wall mutants were obtained by mutagenesis of Saccharomyces cerevisiae X2180-1A, a haploid strain, with N-methyl-N'-nitro-N-nitrosoguanidine.The two S. cerevisiae mutants showed considerable morphological changes and digestibilities to lytic enzymes. Sequential extractions of proteins and carbohydrates from the mutant and wild type cells indicate that the mutants have high protein extractability and lack some wall proteins as well as carbohydrate components.Chemical structures of cell wall mannans and <beta>-glucans from the mutants were studied. Structural analysis of mutant mannans indicated that both N3 and N3-8 mutant mannans lacked-1,2-mannosyl side chains. Structures of alkali-soluble <beta>-glucans from the mutant cells were also different from that of wild type cells in the ratio of 1,6-linkages to 1,3-linkages. The mutant glucans have much higher ratios of <beta>-1,6-linkages to 1,3-linkages.

通过用N-甲基N'N'N硝基N-硝基瓜烷氨酸刺激性X2180-1A的葡萄糖X2180-1A的诱变获得酵母细胞壁突变体。两个酿酒酵母突变体表现出可观的形态学变化和消化能力。从突变体和野生型细胞中蛋白质和碳水化合物的顺序提取表明，突变体具有高蛋白质的可萃取性，缺乏一些壁蛋白以及碳水化合物成分。细胞壁甘露群和<beta> -glucans的化学结构研究了突变体的<beta> -Glucans。突变曼纳人的结构分析表明，N3和N3-8突变体曼纳斯都缺乏1,2-甘露糖基侧链。来自突变细胞的碱性溶质<beta> - 葡聚糖的结构也与野生型细胞的结构不同，其比率为1,6-链接与1,3-链接。突变葡萄糖的比率更高，<beta> -1,6-链接与1,3-链接的比率更高。

项目成果

Nakajima, Tasuku: "Structure and function of yeast cell wall polysaccharides." J. Brew. Soc. Japan.82. 598-605 (1987)

Nakajima，Tasuku：“酵母细胞壁多糖的结构和功能。”

Niura, Nozomi. Nakajima, Tasuku. Matsuda, Kazuo.: "Purification and some properties of an endo-<beta>-1,6-glucanase from Neurospora crassa." Agric. Biol. Chem.51. 3315-3321 (1987)

新浦、希美。

中島佑: 化学と生物. 25. 370-375 (1987)

中岛佑：化学与生物学。25. 370-375 (1987)

N.Hrura;T.Nakajima;K.Matsuda: Agric Biol Chem. 51. 3315-3321 (1987)

N.Hrura；T.Nakajima；K.Matsuda：农业生物化学。

Nakajima,tasuku. Konno,reiko. Nishihara,hiroshi. Matsuda,kazuo.: "Cell wall mutants of Saccharomyces cerevisiae which show remarkable increase of digestibility to cell wall lytic enzyme and of protein extractability." J. Ferment. Technol.66. in press (198

中岛，太久。