A studv on the structure and function of a fungal β-1, 6-glucanase

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A gene (neg1) encoding endo-β-1,6-glucanase from Neurospora crassa was cloned. The putative neg1 was 1443-bp long and encoded a mature endo-β-1,6-glucanpse protein of 463 amino acids and signal peptide of 17 amino.acids. The purified recombinant protein (Neg1) obtained from Escherichia coli showed β-1,6-D-glucanase activity. No homologous genes are found in yeasts. and fungi.The β-1,6-glucanase gene (neg1) was disrupted by using repar-induced point mutation(RIP). The gene disruptant strain (R12-1) had no β-1,6-glucanase activity. The phenotype analyses showed thet the sisuptant had no apparent morphological changes, but protein denaturing agents, such as SDS or CTAB inhibited the mycerial growth.