Changes of the Cell Wall Polysaccharide Structures Associated with Fungal Morpholo

真菌形态相关细胞壁多糖结构的变化

• 批准号: 01560111
• 负责人: NAKAJIMA Tasuku
• 金额: $ 1.22万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560111/
• 关键词: Fungal cell wall; beta-glucan; Neurospora crassa; Cell morphology; Cell wall polysaccharide; Fungal morphology; beta-1, 3-glucan; beta-glucan metabolism; βー1,3ーグルカン; 真菌細胞壁; βー1,3ーグルカナ-ゼ; B-グルカン; Newrospora crassa; 多糖

Cell wall beta-glucans are thought to be one of the major determinants of fungal morphology. To know the developmental changes of beta-glucan structures in Neurospora crassa, alkali-soluble beta-glucans were extracted from spores and from mycelia of four different growth stagesl N sodium hydroxide in the presence of sodium borohydride. Molecular weight determination by gel filtration on Sepharose CL-6B column indicated that the size of the major beta-glucan component in spore wall being 23Kd which decreased to 13kd with the mycelial development. The spore glucan was separated to two molecular species whose molecular weight was 23kd and 13kd, and the mycelial cell wall glucan was separated to four molecular species whose size were 23kd, 13kd, 6kd and 1.7kd, respectively. Methylation analysis of the two beta-glucan components of spore cell walls showed that the 23kd glucan had 84 % of 1,3-linkages with 8 % of 1,6 and 4 % of 1,3-1,6-lnkages, and the 13kd glucan had 91 % of 1,3-linkages with 3 % of 1,6 and 3 % of 1,3-1,6-linkages.

细胞壁β-葡聚糖被认为是真菌形态的主要决定因素之一。为了了解粗枝脉孢霉β-葡聚糖结构的发育变化，在硼氢化钠存在下，从4个不同生长阶段的N-氢氧化钠的孢子和菌丝体中提取了碱溶β-葡聚糖。经SepharoseCL-6B凝胶过滤测定，孢子壁的主要β-葡聚糖组分的大小为23kd，随着菌丝的发育，其大小逐渐减小到13kd。分离得到的孢子葡聚糖分子量分别为23kd和13kd，菌丝细胞壁葡聚糖分子量分别为23kd、13kd、6kd和1.7kd。孢子细胞壁两种β-葡聚糖组分的甲基化分析表明，23kd的葡聚糖含有84%的1，3-键，8%的1，6和4%的1，3-1，6-键；13kd的葡聚糖有91%的1，3-键，3%的1，6和3%的1，3-1，6-键。

项目成果

Tasuku Nakajima el al.: "Changes of the cell wall beta-glucan structures associated with cell development in Neurospora crassa" J. Agric. Chem. Soc.vol. 64. 521 (1990)

Tasuku Nakajima 等人：“与粗糙脉孢菌细胞发育相关的细胞壁 β-葡聚糖结构的变化”J. Agric。

Chiba Yogo: "Two groups of intracellular αーamylase isozymase from cultured rice cells." Phytochemistry. 29. 2075-2078 (1990)

Chiba Yogo：“来自培养水稻细胞的两组细胞内 α-淀粉酶同工酶。” 植物化学 29. 2075-2078 (1990)

中島佑: "グリコ-ゲン生合成の初発機構" 生化学. 62. (1990)

Yu Nakajima：“糖原生物合成的初始机制”生物化学62。（1990）

中島 佑: "グリコ-ゲン生合成の初発機構" 生化学. 62. 125-128 (1990)

Yu Nakajima：“糖原生物合成的初始机制”生物化学 62. 125-128 (1990)。

KASAHARA Shin: "Chemical properties of exoーβー1,3ーglucanase from Aspergillus saitoi." J.Biochem.108. (1991)

KASAHARA Shin：“斋藤曲霉的外切-β-1,3-葡聚糖酶的化学特性。J.Biochem.108（1991）。