Studies of the hormone-dependent expression of conalbumin gene

伴清蛋白基因激素依赖性表达的研究

• 批准号: 61560096
• 负责人: HIROSE Masaaki
• 金额: $ 1.22万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560096/
• 关键词: Conalbumin; ホルモン; レセプター

Conalbumin, an egg white protein, is induced in the chick oviduct by the administration of estrogen. Steroid hormones including estrogen bind to specific receptors in target cells, then the steroid-receptor complexes exert the stimulation effects through their binding to nuclear chromatin. In this study, the role of nuclear histone acetylation was studied. Female chicks were stimulated for 14 to 19 days with estrogen, then oviducts were removed. Nuclear histone was acetylated by incubating the tissue with ^3H-sodium acetate. The release of acetylated histones from chick oviduct chromatin was analyzed by hydroxylapatite column chromatography. By raising of the NaCl concentration, acetylated histones were eluted from hydroxylapatite-bound chromatin depending on their release from nucleosomal DNA. Electrophoresis on acid-urea gel showed that hypoeracetylated forms of histone H4 were eluted at a lower NaCl concentration than non-acetylated or hypoacetylated H4, indicating that hyperacetylated H4 has decreased stability in nucleosomes. These data strongly suggest that histone acetylation has an important role on the gene expression in the chick oviduct.

伴清蛋白是一种卵白色蛋白，在鸡输卵管中被雌激素诱导产生。包括雌激素在内的甾体激素与靶细胞内的特异性受体结合，然后甾体激素-受体复合物通过与核染色质结合而发挥刺激作用。在这项研究中，核组蛋白乙酰化的作用进行了研究。雌性雏鸡用雌激素刺激14 ~ 19天，然后切除输卵管。将组织与^3H-乙酸钠孵育，使核组蛋白乙酰化。用羟基磷灰石柱层析法分析鸡输卵管染色质中乙酰化组蛋白的释放。通过提高NaCl浓度，乙酰化组蛋白从羟基磷灰石结合的染色质中洗脱，这取决于它们从核小体DNA中的释放。酸-尿素凝胶电泳显示，hypoeracetylated形式的组蛋白H4洗脱在较低的NaCl浓度比非乙酰化或hypoacetylated H4，表明hypoacetylated H4在核小体中的稳定性降低。这些数据有力地表明，组蛋白乙酰化对鸡输卵管中的基因表达具有重要作用。

项目成果

M.Hirose: Journal of Biochemistry. 103. 31-35 (1988)

M.Hirose：生物化学杂志。

M.Hirose;N.Takahashi;H.Oe;E.Doi: Analytical Biochemistry. 168. 193-201 (1988)

M.Hirose；N.Takahashi；H.Oe；E.Doi：分析生物化学。

HIROSE, Masaaki: Biochemical response to hormone action in mammary gland nuclei (in "Molecular mechanism of biosignal transduction", edited by Institute of Endocrinology, Gunma University). Center for Academic Publications Japan, Tokyo & VNU Science Press

HIROSE，Masaaki：乳腺细胞核中激素作用的生化反应（《生物信号转导的分子机制》，群马大学内分泌研究所编辑）。

Masaaki Hirose ed.Y.Sakamoto;F.Isohashi: "Glucocorticoid Hormone-Mechanism of Action" Japan Scientific Society Press(Tokyo)& Springer-Verlag(Berlin etc.), 193(15) (1986)

Masaaki Hirose ed.Y.Sakamoto;F.Isohashi：“糖皮质激素-作用机制”日本科学会出版社（东京）

Masaaki Hirose ed.Gunma Univ.: "Molecular Mechanism of Biosignal Transduction" Center for Academic Publications Japan(Tokyo)& VNU Science Press BV(Utrecht)Institute of Endocrinology,180(11) (1986)

广濑正明主编：群马大学：“生物信号转导的分子机制”日本学术出版中心（东京）