Studies on palatability of artificial feed for cultured fish.

养殖鱼类人工饲料的适口性研究。

• 批准号: 61560211
• 负责人: HIDAKA Iwao
• 金额: $ 1.47万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560211/
• 关键词: Fish; Feeding stimulants; Extracts; Amino acids; Nucleotides; Taste; 嗅覚

The feeding stimulatory effects of the extracts of organisms on the yellowtail, Seriola quinqueradiata, and ayu, Plecoglossus altivelis, were studied by offering the extractive substances in satarch or in a purified diet. The stimulatory effects on the taste and olfactory receptors were also studied.Yellowtail:(1) A synthetic mixture of extractive constituents of the mantle muscle of squid prepared based on reported analyses was eaten by the fish as equally as the natural extract of squid muscle when offered in starch. (2) Omission tests on the constituents of the synthetic mixture were performed dividing them into five groups. The results suggested that amino acids and nucleotides effective for the taste receptors might largely be responsible for the feeding stimulant activity of the squid extract. (3) The responses from the olfactory bulb on application of the above 5 groups of constituents to the olfactory epithelium did not consist with the results of the feeding experiments, showi … More ng the high sensitivity of the olfactory receptors to amino acids which were non feeding stimulatory. However, the response threshold of the olfctory receptors for the synthetic mixture appeared to be by 3 log units lower than that of the taste receptors. (4) The synthetic mixure for the jack mackerel muscle extract prepared based on reported analyses was less effective in feeding stimulation than the natural extract, suggesting that some potent substance(s) were missing in the mixture.Ayu fish:(1) The sensitivity of the taste receptors of the ayu fish to extractive substances was studied by recording the taste nerve responses. The taste receptors of this fish, like those of many other fishes reported, responded well to amino acids. Among the most effective at 10^<-2>M were Arg, His,phosphoserine,etc. The threshold for Arg and His was 10^<-6>-10^<-5>M.They also responded well to nucleotides such as AMP,CMP,etc. (2) Attached algae collected in the river were analysed. Compositions of ninhydrin reactive substances (NRS) in algae extract differed considerably with the extracting methods used. Among the most abundant amino acids were Ala,Glu,Gly,Val,etc.(3) Feed consumptions for 10 days in duplicate test groups of ayu fish were compared with control groups. As the results, 1) addition of freeze-dried algae to a purified diet showed the highest comsumption, 2) addition of water extract from algae showed about 70% effectiveness of that of the algae,and 3) amino acids mixture simulating NRS's composition of the algae extract showed only a weak effectiveness. Less

通过在梭子鱼体内或在净化饲料中提供生物提取物，研究了生物提取物对黄尾鱼Seriola quinqueradiata和香鱼Plecoglossus altivelis的摄食刺激作用。黄尾鱼：（1）鱿鱼外套膜肌肉提取物成分的合成混合物是根据报道的分析制备的，当以淀粉形式提供时，与鱿鱼肌肉的天然提取物一样被鱼食用。(2)对合成混合物的成分进行省略测试，将其分为五组。结果表明，鱿鱼提取物的摄食刺激活性主要是由对味觉感受器有效的氨基酸和核苷酸引起的。(3)嗅球对上述5组成分作用于嗅上皮的反应与摄食实验结果不一致，表明上述5组成分对嗅上皮的作用与摄食实验结果不一致。 ...更多信息 嗅觉感受器对非摄食刺激性氨基酸的敏感性较高。然而，嗅觉受体对合成混合物的反应阈值似乎比味觉受体低3个对数单位。(4)根据报道的分析制备的竹荚鱼肌肉提取物的合成混合物在摄食刺激方面不如天然提取物有效，这表明混合物中缺少一些有效物质。这种鱼的味觉受体，像许多其他鱼类的报告，反应良好的氨基酸。在10 μ M浓度下，最有效的<-2>有Arg、His、磷酸丝氨酸等，Arg和His的阈值为10 <-6>~ 10 μ <-5>M，它们对AMP、CMP等核苷酸也有较好的反应。不同提取方法的海藻提取物中茚三酮反应性物质（NRS）的组成有很大差异。其中含量最高的氨基酸为Ala、Glu、Gly、瓦尔等。(3)对两个重复试验组和对照组香鱼10天的摄食量进行了比较。结果表明：（1）在纯化饲料中添加冻干藻的效果最好;（2）添加藻水提取物的效果约为藻水提取物的70%;少

项目成果

帝釈元,新井茂,日高磐夫: 昭和62年度日本水産学界春季大会口演発表(1987.4.2). (1987)

Gen Taishaku、Shigeru Arai、Iwao Hidaka：在 1987 年日本水产学会春季会议上的口头报告（1987 年 4 月 2 日）（1987 年）。

Iwao HIDAKA and Yoshinari ISHIDA: "Taste responses in marine teleosts." Proc. 20th Jap. Symp. Taste and Smell. 20. 93-96 (1986)

Iwao HIDAKA 和 Yoshinari ISHIDA：“海洋硬骨鱼的味觉反应。”

ISHIDA YOSHINARI,HIDAKA IWAO: Nippon Suisan Gakkaishi. 53. (1987)

石田吉成、岩尾日高：日本水产学会。

福田一弥,日高磐夫,神原淳: 昭和62年度日本水産学会春季大会.

Kazuya Fukuda、Iwao Hidaka、Jun Kambara：1985 年日本水产科学会春季会议。

神原淳,福田一弥,日高磐夫: 昭和63年度日本水産学界春季大会口演発表予定(1988.4.4). (1988)

Jun Kambara、Kazuya Fukuda、Iwao Hidaka：计划在 1988 年日本水产学会春季会议上进行口头报告（1988 年 4 月 4 日）（1988 年）。