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Studies on stimulation of feeding behavior in yellowtail Seriola quinqueradiata.

鰤鱼摄食行为刺激的研究。

基本信息

批准号：
07660243
负责人：
HIDAKA Iwao
金额：
$ 1.41万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660243/
关键词：
Fish Yellowtail Feeding stimulants Muscle extracts Taste Olfaction 魚類エキス

项目摘要

The feeding-stimulatory effects on the yellowtail Seriola quinqueradiata of extracts of the jack mackerel Trachurus japonicus white muscle were studied. Ultrafiltrates of water extracts of the muscle (M.W.cut off 10,000) were charged on a DEAE-Sephadex A-25 at pH 5.5 or CM-Sephadex C-25 at pH 6.5. Effluents and eluates with 0.6M NaCl were freeze-dried and the components were applied in starch pellets to yellowtails, 23-31g in body weight. Their feeding-stimulatory effectiveness was evaluated by counting the number of pellets eaten within the first minute after application.Their stimulatory effects on the gustatory and olfactory receptors of the yellowtail, 230-746g in b.w., were also studied by recording the electrical responses from the palatine taste nerve and olfactory bulb.(1) Feeding-stimulatory effectiveness of fractioned components (a) Components of the effluent through DEAE-Sephadex column were ineffective, while those of the eluate with NaCl were slightly less effective than t … More he untreated extract. (b) Stepwise elution with 0.2M and 0.6M NaCl on the DEAE-Sephadex column yielded two less effective clusters of different components : 0.2M NaCl-eluate contained lactic acid and minhydrin positive compounds, and 0.6M NaCl-eluate inosinic acid. (c) Components of the eluate from CM-Sephadex C-25 were ineffective, while those of the effluent were nearly as effective as the total extract.(2) Gustatory and olfactory neural responses to fractioned components (a) Both gustatory and olfactory systems responded more or less to all fractions tested. (b) The threshold for the total extract was lower in olfactory system than in gustatory system by some three orders. That for some fractions was also so to different degrees while that for others was similar to each other between the two systems. (c) The threshold for both of lactic acid and inosinic acid, present in 0.2M and 0.6M NaCl-eluates from DEAE-Sephadex culumn respectively and considered one of important components, was lower in gustatory system. Less

研究了竹荚鱼Trachurus japonicus 白肌提取物对鰤鱼的摄食刺激作用。将肌肉水提取物的超滤液（分子量截止为 10,000）加载到 pH 5.5 的 DEAE-Sephadex A-25 或 pH 6.5 的 CM-Sephadex C-25 上。将含有0.6M NaCl的流出物和洗出液冷冻干燥，并将这些组分以淀粉颗粒的形式施用于体重23-31g的黄尾鱼。通过计算施用后第一分钟内吃下的颗粒数量来评估它们的摄食刺激效果。还通过记录腭味神经和嗅球的电反应来研究它们对黄尾鱼（体重为 230-746 克）的味觉和嗅觉受体的刺激作用。（1）分馏成分的摄食刺激效果（a）成分通过 DEAE-Sephadex 柱的流出液的效果无效，而含有 NaCl 的洗脱液的效果略低于未经处理的提取物。 (b)在DEAE-Sephadex柱上用0.2M和0.6M NaCl逐步洗脱产生不同组分的两个不太有效的簇：0.2M NaCl-洗脱液含有乳酸和茚三酮阳性化合物，和0.6M NaCl-洗脱液肌苷酸。 (c) CM-Sephadex C-25 的洗脱液成分无效，而流出液的成分几乎与总提取物一样有效。 (2) 对分级成分的味觉和嗅觉神经反应 (a) 味觉和嗅觉系统对所有测试的级分或多或少都有反应。 (b)嗅觉系统中总提取物的阈值比味觉系统中的总提取物阈值低约三个数量级。对于某些分数来说，这也是不同程度的，而对于其他分数来说，这两个系统之间的情况是相似的。 (c) 乳酸和肌苷酸分别存在于 DEAE-Sephadex 柱的 0.2M 和 0.6M NaCl 洗脱液中，被认为是重要成分之一，在味觉系统中的阈值较低。较少的