Studies on stimulation of feeding behavior in yellowtail Seriola quinqueradiata.

鰤鱼摄食行为刺激的研究。

基本信息

批准号：
07660243
负责人：
HIDAKA Iwao
金额：
$ 1.41万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660243/
关键词：
Fish Yellowtail Feeding stimulants Muscle extracts Taste Olfaction 魚類エキス

项目摘要

The feeding-stimulatory effects on the yellowtail Seriola quinqueradiata of extracts of the jack mackerel Trachurus japonicus white muscle were studied. Ultrafiltrates of water extracts of the muscle (M.W.cut off 10,000) were charged on a DEAE-Sephadex A-25 at pH 5.5 or CM-Sephadex C-25 at pH 6.5. Effluents and eluates with 0.6M NaCl were freeze-dried and the components were applied in starch pellets to yellowtails, 23-31g in body weight. Their feeding-stimulatory effectiveness was evaluated by counting the number of pellets eaten within the first minute after application.Their stimulatory effects on the gustatory and olfactory receptors of the yellowtail, 230-746g in b.w., were also studied by recording the electrical responses from the palatine taste nerve and olfactory bulb.(1) Feeding-stimulatory effectiveness of fractioned components (a) Components of the effluent through DEAE-Sephadex column were ineffective, while those of the eluate with NaCl were slightly less effective than t … More he untreated extract. (b) Stepwise elution with 0.2M and 0.6M NaCl on the DEAE-Sephadex column yielded two less effective clusters of different components : 0.2M NaCl-eluate contained lactic acid and minhydrin positive compounds, and 0.6M NaCl-eluate inosinic acid. (c) Components of the eluate from CM-Sephadex C-25 were ineffective, while those of the effluent were nearly as effective as the total extract.(2) Gustatory and olfactory neural responses to fractioned components (a) Both gustatory and olfactory systems responded more or less to all fractions tested. (b) The threshold for the total extract was lower in olfactory system than in gustatory system by some three orders. That for some fractions was also so to different degrees while that for others was similar to each other between the two systems. (c) The threshold for both of lactic acid and inosinic acid, present in 0.2M and 0.6M NaCl-eluates from DEAE-Sephadex culumn respectively and considered one of important components, was lower in gustatory system. Less

对黄尾seriola quinqueradiata的喂养刺激作用是jack鲭鱼豆腐japonicus白色肌肉的提取物。在pH 5.5的Deae-Sephadex A-25上，在DEAE-Sephadex A-25上收取了肌肉水提取物的超滤液（M.W.切除10,000）或pH 6.5时CM-Sephadex C-25。将0.6m NaCl的废水和余质冷冻干燥，并将成分涂在淀粉颗粒中，以使体重为23-31g。通过计算施用后的第一分钟，通过计算对刺激性和嗅觉受体的刺激作用，在b.w. 230-746g中对它们的刺激作用进行了研究，从而评估了它们的进食刺激有效性。通过Deae-Sephadex柱的有效组件无效，而NaCl的洗脱器的组件效果略低于……他未经处理的提取物。（b）Deae-Sephadex柱上用0.2m和0.6M NaCl进行逐步洗脱，产生了两个不同组分的有效簇的两个较低的簇：0.2m NaCl-溶于NaCl-含有乳酸和Minhydrin阳性化合物，以及0.6m NaCl-NaCl-NaCl-溶解的含量。（c）来自CM-Sephadex C-25的洗脱体的组件无效，而有效的成分几乎与总提取物一样有效。（2）味觉和嗅觉神经对分馏组件的味觉和嗅觉神经响应（a）味觉和嗅觉系统对所有分数的响应都响应于所有分数。（b）嗅觉系统中总提取物的阈值比在味道系统中低约三个顺序。对于某些分数而言，这在不同程度上也是如此，而对于其他组分，这两个系统之间彼此相似。（c）分别来自Deae-Sephadex culumuln的0.2m和0.6m naCl-释放的乳酸和肌酸的阈值，并且被认为是重要的成分之一，味道较低。较少的