Studies on stimulation of feeding behavior in yellowtail Seriola quinqueradiata.

研究了青花鱼白肌提取物对五角黄尾虫的促食作用。肌肉水提取物的超滤液（m.w.p切10000）在pH 5.5的DEAE-Sephadex a -25或pH 6.5的CM-Sephadex C-25上充电。用0.6M NaCl冷冻干燥出水和洗脱液，将各组分制成淀粉粒，投喂体重23-31g的黄尾鱼。通过计算应用后第一分钟内食用的颗粒数量来评估其喂养刺激效果。通过记录腭味觉神经和嗅球的电反应，研究了它们对体重230 ~ 746g的黄尾鱼味觉和嗅觉受体的刺激作用。(1)经DEAE-Sephadex柱处理的出水组分的进料刺激效果不明显，NaCl处理的洗脱液的进料刺激效果略低于未经处理的萃取物。(b)用0.2M和0.6M NaCl在DEAE-Sephadex柱上逐级洗脱，得到两种不同成分的效果较差的簇：0.2M NaCl洗脱液含有乳酸和甲醇阳性化合物，0.6M NaCl洗脱液含有肌苷酸。(c) CM-Sephadex c -25洗脱液的成分无效，而流出物的成分几乎与总萃取物一样有效。(2)味觉和嗅觉神经对不同组分的反应(a)味觉和嗅觉系统对所有测试组分都或多或少有反应。(b)嗅觉系统对总提取物的阈值比味觉系统低3个数量级。在两个体系中，一些分数的差异程度不同，而另一些分数的差异程度相似。(c)乳酸和肌苷酸在味觉系统中的阈值较低，而乳酸和肌苷酸分别存在于0.2M和0.6M的DEAE-Sephadex培养液中，被认为是重要成分之一。少

The feeding-stimulatory effects on the yellowtail Seriola quinqueradiata of extracts of the jack mackerel Trachurus japonicus white muscle were studied. Ultrafiltrates of water extracts of the muscle (M.W.cut off 10,000) were charged on a DEAE-Sephadex A-25 at pH 5.5 or CM-Sephadex C-25 at pH 6.5. Effluents and eluates with 0.6M NaCl were freeze-dried and the components were applied in starch pellets to yellowtails, 23-31g in body weight. Their feeding-stimulatory effectiveness was evaluated by counting the number of pellets eaten within the first minute after application.Their stimulatory effects on the gustatory and olfactory receptors of the yellowtail, 230-746g in b.w., were also studied by recording the electrical responses from the palatine taste nerve and olfactory bulb.(1) Feeding-stimulatory effectiveness of fractioned components (a) Components of the effluent through DEAE-Sephadex column were ineffective, while those of the eluate with NaCl were slightly less effective than t … More he untreated extract. (b) Stepwise elution with 0.2M and 0.6M NaCl on the DEAE-Sephadex column yielded two less effective clusters of different components : 0.2M NaCl-eluate contained lactic acid and minhydrin positive compounds, and 0.6M NaCl-eluate inosinic acid. (c) Components of the eluate from CM-Sephadex C-25 were ineffective, while those of the effluent were nearly as effective as the total extract.(2) Gustatory and olfactory neural responses to fractioned components (a) Both gustatory and olfactory systems responded more or less to all fractions tested. (b) The threshold for the total extract was lower in olfactory system than in gustatory system by some three orders. That for some fractions was also so to different degrees while that for others was similar to each other between the two systems. (c) The threshold for both of lactic acid and inosinic acid, present in 0.2M and 0.6M NaCl-eluates from DEAE-Sephadex culumn respectively and considered one of important components, was lower in gustatory system. Less