Studies on stimulation of feeding behavior in yellowtail Seriola quinqueradiata.

研究了竹荚鱼白色肌肉提取物对青斑石斑鱼（Seriola quinqueradiata）的促摄食作用。将肌肉的水提取物（截留分子量为10，000）的超声波加载到pH 5.5的DEAE-Sephadex A-25或pH 6.5的CM-Sephadex C-25上。将含有0.6M NaCl的流出物和洗脱液冷冻干燥，并将组分以淀粉颗粒形式施用于体重为23- 31 g的黄尾鱼。它们对黄尾鱼味觉和嗅觉感受器的刺激作用，体重230- 746克，并通过记录腭味觉神经和嗅球的电反应进行研究。(1)（a）DEAE-Sephadex柱层析分离的组分的促摄食作用不明显，而NaCl柱层析分离的组分的促摄食作用略低于DEAE-Sephadex柱层析分离的组分。 关于我们 未处理的提取物。(b)在DEAE-Sephadex柱上用0.2M和0.6M NaCl逐步洗脱，得到两个不同组分的不太有效的簇：0.2M NaCl-葡萄糖，含有乳酸和mindridine阳性化合物，和0.6M NaCl-肌苷酸。(c)从CM-Sephadex C-25的菌丝体的成分是无效的，而那些流出物几乎是有效的总提取物。(2)味觉和嗅觉神经对组分的反应（a）味觉和嗅觉系统对所有测试的组分都或多或少地有反应。(b)总提取物的阈值在嗅觉系统中比在味觉系统中低三个数量级。两种体系中，某些组分的吸附量也有不同程度的差异，而另一些组分的吸附量则基本相同。(c)乳酸和肌苷酸分别存在于DEAE-Sephadex柱的0.2M和0.6M NaCl洗脱液中，是味觉系统的重要成分之一。少

The feeding-stimulatory effects on the yellowtail Seriola quinqueradiata of extracts of the jack mackerel Trachurus japonicus white muscle were studied. Ultrafiltrates of water extracts of the muscle (M.W.cut off 10,000) were charged on a DEAE-Sephadex A-25 at pH 5.5 or CM-Sephadex C-25 at pH 6.5. Effluents and eluates with 0.6M NaCl were freeze-dried and the components were applied in starch pellets to yellowtails, 23-31g in body weight. Their feeding-stimulatory effectiveness was evaluated by counting the number of pellets eaten within the first minute after application.Their stimulatory effects on the gustatory and olfactory receptors of the yellowtail, 230-746g in b.w., were also studied by recording the electrical responses from the palatine taste nerve and olfactory bulb.(1) Feeding-stimulatory effectiveness of fractioned components (a) Components of the effluent through DEAE-Sephadex column were ineffective, while those of the eluate with NaCl were slightly less effective than t … More he untreated extract. (b) Stepwise elution with 0.2M and 0.6M NaCl on the DEAE-Sephadex column yielded two less effective clusters of different components : 0.2M NaCl-eluate contained lactic acid and minhydrin positive compounds, and 0.6M NaCl-eluate inosinic acid. (c) Components of the eluate from CM-Sephadex C-25 were ineffective, while those of the effluent were nearly as effective as the total extract.(2) Gustatory and olfactory neural responses to fractioned components (a) Both gustatory and olfactory systems responded more or less to all fractions tested. (b) The threshold for the total extract was lower in olfactory system than in gustatory system by some three orders. That for some fractions was also so to different degrees while that for others was similar to each other between the two systems. (c) The threshold for both of lactic acid and inosinic acid, present in 0.2M and 0.6M NaCl-eluates from DEAE-Sephadex culumn respectively and considered one of important components, was lower in gustatory system. Less