Studies on stimulation of feeding behavior in yellowtail Seriola quinqueradiata

鰤鱼摄食行为刺激研究

• 批准号: 03660190
• 负责人: HIDAKA Iwao
• 金额: $ 1.34万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660190/
• 关键词: Fish; Yellowtail; Feeding stimulants; Extracts; Taste

The feeding-stimulatory effectiveness of a water extract of the jack mackerel Trachurus japonicus white muscle for the young yellowtail Seriola quinqueradiata was studied with the aid of gel chromatography, ion exchange chromatography and other analytical methods. The feeding-stimulatory effectiveness of test fractions of the extract was evaluated by applying them to the young yellowtail in starch pellets and counting the number of pellets eaten by the fish. (1) Gel fractions of the extracts on a column of Sephadex-15 The water extract of the muscle was ultrafiltrated through a filter membrane (UK-15, Advantic, M.W. cut off 10,000) and the ultrafiltrate was fractionated on a column of Sephadex G-15 from 0.05 M sodium chloride. The fractions obtained were divided into groups and pooled. Then the pooled eluate was freeze-dried and the residues were dissolved in sea water and applied to the fish. The results showed that effective compounds were included in fractions of molecular weight of several hundreds or lower. (2) Fractions of the extracts obtained by treating with DEAE-Sephadex and CM-Sephadex The ultrafiltrate of the muscle extract was treated with (a) DEAE-Sephadex A-25 anion exchanger, (b) CM-Sephadex C-25 cation exchanger or (c) both of the two ion exchangers in batch procedures. The results obtained showed that (a) reduced the stimulatory effectiveness of the extract. This suggests that some of the effective constituents are adsorbed to the DEAE-Sephadex;(b) had no apparent influence on the effectiveness of the extract; (c) resulted in a sever depression of the effectiveness, suggesting that constituents adsorbing to the cation exchanger also show a feeding- stimulatory effect in cooperation with constituents in the (c)-treated extract.

采用凝胶层析、离子交换层析等分析方法，研究了日本青鱼白肌肉水提物对五叶黄尾幼体的促食作用。通过将其应用于淀粉颗粒中的幼年黄尾鱼，并计算其摄食颗粒的数量，来评价该提取物的试验部分的促食效果。(1)Sephadex-15柱上提取物的凝胶部分肌肉的水提取物通过滤膜(UK-15，Advantic，M.W.截留10,000)超滤，超滤液在Sephadex G-15柱上从0.05M氯化钠中分级。将得到的组分分组并汇集在一起。然后将混合的洗脱液冷冻干燥，残留物溶解在海水中并应用于鱼。结果表明，有效化合物存在于分子量在几百或更低的级分中。(2)DEAE-Sephadex和CM-Sephadex处理提取物的部分。肌肉提取物的超滤液用(A)DEAE-Sephadex A-25阴离子交换剂、(B)CM-Sephadex C-25阳离子交换剂或(C)两种离子交换剂分批处理。结果表明：(A)降低了提取物的刺激性。这表明部分有效成分被吸附在DEAE-Sephadex上；(B)对提取物的效力没有明显的影响；(C)导致效力的严重抑制，这表明吸附在阳离子交换器上的成分也与(C)处理的提取物中的成分一起显示出促进摄食的作用。

项目成果

日高 磐夫他,25名分担執筆: "魚類生理学(第15章 「味覚」を担当)" 恒星社厚生閣, 621 (1991)

Iwao Hidaka 和其他 25 人合着：“鱼类生理学（第 15 章：味道”），Seiseisha Koukaku，621 (1991)

I. Hidaka: "Taste." In "Fish Physiology" (ed. by Y. Itazawa and I. Hanyu), Koseishakoseikaku, Tokyo. 489-518 in Japanese (1991)

I.日高：“尝尝。”

Iwao Hidaka Ceng Zeng Jun Kohbra: "Gustatory responses to organic acids in the yellowtail Seriola quinqueradiata" Nippon Suisan Gakkaishi. 58. 1179-1187 (1992)

Iwao Hidaka Ceng Zeng Jun Kohbra：“黄尾鰤有机酸的味觉反应”Nippon Suisan Gakkaishi。

Iwao HIDAKA,Ceng Zeng,Jun KOHBARA: "Gustatory responses to organic acids in the yellowtail seriola quinqueradita" Nippon Suisan Gakkaishi. 58. 1179-1187 (1992)

Iwao HIDAKA、Ceng Zeng、Jun KOHBARA：“鰤鱼有机酸的味觉反应”Nippon Suisan Gakkaishi。

Jun Kohbara Iwao Hidaka Tatsuo Morishita: "The feeding-stimulatory effectioveness of L-lactic acid on the young yellowtail Seriola quinqueradiata" Nippon Suisan Gakkaishi. 59. 183- (1993)

Jun Kohbara Iwao Hidaka Tatsuo Morishita：“L-乳酸对幼年鰤鱼的摄食刺激作用”Nippon Suisan Gakkaishi。