PHYSIOLOGICAL ROLE OF SHORT-CHAIN FATTY ACIDS IN CONTROL OF PANCREATIC EXOCRINE SECRETION OF RUMINANTS.

据报道，短链脂肪酸能刺激绵羊的胰腺外分泌。然而，目前还不清楚这种效应是否在控制胰腺分泌方面起到了生理作用。本研究旨在阐明绵羊在生理条件下胰腺外分泌对SHORTCHAIN脂肪酸的反应。1.采用顶空气相色谱分析血液中的短链脂肪酸。2.喂饲2小时后血浆中醋酸盐浓度为2458；+-149unol/1.3。3.静脉滴注醋酸钠(pH：7.4)12.5、25、50和75微克/公斤分，可引起胰腺外分泌反应增强，但输注期血浆中的乙酰胆碱浓度低于各给药剂量下的给药后浓度。4.随着短链脂肪酸碳数的增加，胰腺分泌反应增强。5.静脉注射CCK-8和静脉输注醋酸盐对胰腺的反应低于不输注。相比之下，输注醋酸盐对促胰液素的反应比不输注促胰液素的反应更大。6.静脉注射丁酸对胰腺外分泌有明显的抑制作用。7.HCO_3对胰液的分泌作用为分泌等效丁酸。8.不同部位、不同部位给予短链脂肪酸后，胰腺外分泌反应不同。

IT IS REPORTED THAT THE SHORT-CHAIN FATTY ACIDS STIMULATE THE PANCREATIC EXOCRINE SECRETION IN SHEEP. HOWEVER, IT IS UNCLEAR WHETHER THIS EFFECT PLAYS PHYSIOLOGICAL ROLE IN CONTROL OF PANCREATIC SECRETION. THE PRESENT STUDY WAS UNDERTAKEN TO CLARIFY THE RESPONSE OF PANCREATIC EXOCRINE SECRETION TO SHORTCHAIN FATTY ACIDS UNDER THE PHYSIOLOGICAL CONDITION IN SHEEP. 1.SHORT-CHAIN FATTY ACIDS IN BLOOD WERE ANALYZED BY THE HEAD-GAS-SPACE GAS CHROMATOGRAPHY. 2.THE CONCENTRATION OF ACETATE IN PLASMA 2hrs AFTER FEEDING WAS 2458<plus-minus>149unol/1. 3.INTRAVENOUS INFUSION OF Na-ACETATE(pH:7.4) AT THE RATE OF 12.5, 25, 50 AND 75 <micro> mol/kg.min RESULTED IN INCREASED RESPONSE OF PANCREATIC EXOCRINE SECRETION, THOUGH THE ACTETATE CONCENTRATION IN PLASMA DURING INFUSION PERIOD WAS LOWER THAN THE VALUE AFTER FEEDING IN EVERY ADMINISTRATION RATES. 4.THE RESPONSE OF PANCREATIC SECRETION WAS INCREASED AS INCREASING NUMBER OF CARBON OF SHORT-CHAIN FATTY ACID. 5.THE PANCREATIC RESPONSE TO THE INTRAVENOUS ADMINISTRATION OF CCK-8 WITH INTRAVENOUS INFUSION OF ACETATE WAS LESS THAN THAT WITHOUT INFUSION. IN CONTRAST, THE RESPONSE TO SECRETIN WAS BIGGER WITH ACETATE INFUSION THAN WITHOUT ONE. 6.A MARKED INHIBITION OF PANCREATIC EXOCRINE SECRETION APPEARED AFTER THE STIMULATION BY INTRAVENOUS INJECTION OF BUTYRATE. 7.THE SECRETORY EFFECT OF HCO_3 INTO PANCREATIC JUICE WAS SECRETIN > CCK-8 <similar equal> BUTYRATE. 8.THE ADMINISTRATION OF SHORT-CHAIN FATTY ACIDS INTO THE DIFFERENT SITE, INTRARUMINALLY, INTRADUODENALLY OR INTRAVENOUSLY RESULTED IN DIFFERENT RESPONSES OF PANCREATIC EXOCRINE SECRETION.