PHYSIOLOGICAL ROLE OF SHORT-CHAIN FATTY ACIDS IN CONTROL OF PANCREATIC EXOCRINE SECRETION OF RUMINANTS.

短链脂肪酸在控制反刍动物胰腺外分泌分泌中的生理作用。

• 批准号: 61560314
• 负责人: KATO SEIYU
• 金额: $ 0.96万
• 依托单位: RAKUNO GAKUEN UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560314/
• 关键词: Sheep; Pancreatic Exocrine Secretion; 短鎖脂肪酸; 血漿短鎖脂肪酸

IT IS REPORTED THAT THE SHORT-CHAIN FATTY ACIDS STIMULATE THE PANCREATIC EXOCRINE SECRETION IN SHEEP. HOWEVER, IT IS UNCLEAR WHETHER THIS EFFECT PLAYS PHYSIOLOGICAL ROLE IN CONTROL OF PANCREATIC SECRETION. THE PRESENT STUDY WAS UNDERTAKEN TO CLARIFY THE RESPONSE OF PANCREATIC EXOCRINE SECRETION TO SHORTCHAIN FATTY ACIDS UNDER THE PHYSIOLOGICAL CONDITION IN SHEEP. 1.SHORT-CHAIN FATTY ACIDS IN BLOOD WERE ANALYZED BY THE HEAD-GAS-SPACE GAS CHROMATOGRAPHY. 2.THE CONCENTRATION OF ACETATE IN PLASMA 2hrs AFTER FEEDING WAS 2458<plus-minus>149unol/1. 3.INTRAVENOUS INFUSION OF Na-ACETATE(pH:7.4) AT THE RATE OF 12.5, 25, 50 AND 75 <micro> mol/kg.min RESULTED IN INCREASED RESPONSE OF PANCREATIC EXOCRINE SECRETION, THOUGH THE ACTETATE CONCENTRATION IN PLASMA DURING INFUSION PERIOD WAS LOWER THAN THE VALUE AFTER FEEDING IN EVERY ADMINISTRATION RATES. 4.THE RESPONSE OF PANCREATIC SECRETION WAS INCREASED AS INCREASING NUMBER OF CARBON OF SHORT-CHAIN FATTY ACID. 5.THE PANCREATIC RESPONSE TO THE INTRAVENOUS ADMINISTRATION OF CCK-8 WITH INTRAVENOUS INFUSION OF ACETATE WAS LESS THAN THAT WITHOUT INFUSION. IN CONTRAST, THE RESPONSE TO SECRETIN WAS BIGGER WITH ACETATE INFUSION THAN WITHOUT ONE. 6.A MARKED INHIBITION OF PANCREATIC EXOCRINE SECRETION APPEARED AFTER THE STIMULATION BY INTRAVENOUS INJECTION OF BUTYRATE. 7.THE SECRETORY EFFECT OF HCO_3 INTO PANCREATIC JUICE WAS SECRETIN > CCK-8 <similar equal> BUTYRATE. 8.THE ADMINISTRATION OF SHORT-CHAIN FATTY ACIDS INTO THE DIFFERENT SITE, INTRARUMINALLY, INTRADUODENALLY OR INTRAVENOUSLY RESULTED IN DIFFERENT RESPONSES OF PANCREATIC EXOCRINE SECRETION.

据报道，短链脂肪酸刺激绵羊胰腺外分泌。然而，这种效应是否在控制胰腺分泌中发挥生理作用尚不清楚。本研究旨在探讨生理条件下绵羊胰腺外分泌对短链脂肪酸的反应。1.采用顶空气相色谱法测定血液中的短链脂肪酸。2.饲喂后2小时血浆中的乙酸盐浓度为2458 <plus-minus>× 149 unol/l。3.以12.5、25、50和75 mol/kg·min的速率静脉输注醋酸钠（pH：7.4）<micro>，可使胰腺外分泌反应增强，但输注期间血浆中醋酸钠浓度在各给药速率下均低于进食后的值。4.胰腺分泌反应随短链脂肪酸碳数的增加而增加。5.静脉注射CCK-8并静脉输注醋酸盐的胰腺反应低于不输注的胰腺反应。最后，醋酸盐输注对促分泌素的反应比不输注更大。6.丁酸盐静脉注射后，胰腺外分泌出现明显抑制。7. HCO_3对胰液的毒性作用为分泌素&gt; CCK-8<similar equal>丁酸盐。8.短链脂肪酸在不同部位的给药（腔内、十二指肠内或静脉内）对胰腺外分泌的反应不同。

项目成果

KATO, S.: "THE EFFECT OF INTAVENOUS ADMINISTRATION OF SHORT-CHAIN FATTY ACIDS ON PANCREATIC EXOCRINE SECRETION IN CONCIOUS SHEEP." JPN.J.ZOOTECH.SCI.

KATO, S.：“短链脂肪酸对意识绵羊胰腺外分泌分泌的影响。”

加藤清雄: 日本畜産学会報.

加藤清雄：日本动物科学会公报。

KATO, S.: "THE RELATIONSHIP BETWEEN BLOOD ACETATE CONCENTRATION AND PANCREATIC EXOCRINE SECRETION IN SHEEP." JPN.J.ZOOTECH.SCI.

KATO, S.：“绵羊血醋酸浓度与胰腺外分泌分泌之间的关系。”

KATO, S.: "THE EFFECTS OF INTRARUMINAL, INTRADUODENAL AND INTRAVENOUS ADMINISTRATION OF SODIUM ACETATE ON PANCREATIC EXOCRINE SECRETION IN SHEEP." JPN.J.ZOOTECH.SCI.

KATO, S.：“瘤胃内、十二指肠内和静脉内施用乙酸钠对绵羊胰腺外分泌分泌的影响。”