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Ultrastructural alterations of the coronary atrety induced by arterial spasm.

动脉痉挛引起的冠状动脉超微结构改变。

基本信息

批准号：
61570022
负责人：
FUJIMOTO Sunao
金额：
$ 1.09万
依托单位：
University of Occupational and Environmental Health,School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Remarkable spasms of in-vitro swine coronary arteries immersed in Kreds Ringer were induced by application 3f histamine(10^<-3>M) and acetylcholine (10^<-3>M) but not by ergonovine,prostablandine _<2<alpha>>serotonin,and noradrenaline. In crder to observe the morphological changes of the arterial wall especially of the tunica intima during spasmic phases induced by histamine,the Ringer solution was promptly replaced by Karnovskey's fixatives.Intercellular clefts of the endothelia are extensively dilated such an extent that the intercellular vacuoles exist between the endothelial cells.Exfoliation of the endothelial cells from the tunica intima occurs in the limited regions.Some endothelial cells possess large intracellular vacuoles including various cell debrises.Furthermore,increase in the endothelial permeability is evident from marker experiments using HRP.The present observation indicates significant morphological damages occur in the tunica intima especially in the endothelia during the histamine-mediated coronary spasm.Such damages may increase in turn the endothelial permeability,and such sequences may multiply the coronary spasm.Slight spasms of the swine coronary artery were also induced by application of compound 48/80 (1mg/ml Ringer), a histamine liberator.Electron microscopy reveals a marked degranulation of endothelial specific granules (Weibel-Palade bodies) by this drug as in the case of the other vessels. Since these granules have been considered to be histamine reservoir sites,it is reasonable to consider that the arterial spasms by compound 48/80 are due to histamine mediated contraction of the tunica media.

应用组胺（10 μ M）和乙酰胆碱（10 μ M）可引起浸在Kreds Ringer液中的离体猪冠状动脉的显著痉挛<-3><-3>，而麦角新碱、前列腺素<2 > 5<alpha>-羟色胺和去甲肾上腺素则不能引起痉挛。为观察组胺引起的痉挛期动脉壁尤其是图尼卡内膜的形态学变化，立即用Karnovskey固定液代替Ringer液，内皮细胞间裂隙扩大，内皮细胞间存在空泡，内皮细胞从内膜图尼卡中脱落，部分内皮细胞组胺介导的冠状动脉痉挛时，内膜图尼卡尤其是内皮细胞发生了明显的形态学损伤，这种损伤可导致内皮细胞通透性增加，应用化合物48/80也可引起猪冠状动脉轻微痉挛（1 mg/ml林格氏液），一种组胺释放剂。电子显微镜显示内皮细胞特异性颗粒明显脱颗粒（韦伯-帕拉德体）与其他血管一样。由于这些颗粒被认为是组胺储库部位，因此可以合理地认为化合物48/80引起的动脉痉挛是由于组胺介导的图尼卡中膜收缩。